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Mouse Anti-PEG10 Recombinant Antibody (1B1C4) (CBMAB-P1425-YC)

Provided herein is a Mouse monoclonal antibody against Human Paternally Expressed 10. The antibody can be used for immunoassay techniques, such as ELISA, IF, IHC-P, WB.
See all PEG10 antibodies
Published Data
Fig.1 Representative PEG10 IHC staining and quantification in adrenal metastases.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1B1C4
Antibody Isotype
IgG1
Application
ELISA, IF, IHC-P, WB

Basic Information

Immunogen
Purified recombinant fragment of PEG10(aa1-120) expressed in E. coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Paternally Expressed 10
Introduction
PEG10 is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by-1 translational frameshifting (-1 FS). While-1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of-1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for-1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene.
Entrez Gene ID
23089
UniProt ID
Q86TG7
Alternative Names
Paternally Expressed 10; Retrotransposon Gag Domain-Containing Protein 3; Retrotransposon-Derived Gag-Like Polyprotein; Mammalian Retrotransposon-Derived Protein 2; Myelin Expression Factor 3-Like Protein 1; Sushi-Ichi Retrotransposon Homolog 1; Paternally Expressed Gene 10 Protein; Mammalian Retrotransposon-Derived 2; Retrotransposon Gag Like 2; Ty3/Gypsy-Like Protein; MART2; RGAG3; Mar2;
Function
Retrotransposon-derived protein that binds its own mRNA and self-assembles into virion-like capsids (PubMed:34413232).
Forms virion-like extracellular vesicles that encapsulate their own mRNA and are released from cells, enabling intercellular transfer of PEG10 mRNA (PubMed:34413232).
Binds its own mRNA in the 5'-UTR region, in the region near the boundary between the nucleocapsid (NC) and protease (PRO) coding sequences and in the beginning of the 3'-UTR region (PubMed:34413232).
Involved in placenta formation: required for trophoblast stem cells differentiation (By similarity).
Involved at the immediate early stage of adipocyte differentiation (By similarity).
Overexpressed in many cancers and enhances tumor progression: promotes cell proliferation by driving cell cycle progression from G0/G1 (PubMed:12810624, PubMed:16423995, PubMed:26235627, PubMed:28193232).
Enhances cancer progression by inhibiting the TGF-beta signaling, possibly via interaction with the TGF-beta receptor ACVRL1 (PubMed:15611116, PubMed:26235627, PubMed:30094509).
May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter; additional evidences are however required to confirm this result (By similarity).
Biological Process
Apoptotic processIEA:UniProtKB-KW
Cell differentiationIEA:UniProtKB-KW
mRNA transportISS:UniProtKB
Negative regulation of transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:HGNC-UCL
Protein homooligomerizationISS:UniProtKB
Vesicle-mediated intercellular transportISS:UniProtKB
Cellular Location
Extracellular vesicle membrane
Cytoplasm
Nucleus
Forms virion-like extracellular vesicles that are released from cells (PubMed:34413232).
Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the Hep-G2 cell line (PubMed:12810624).
PTM
Isoform 1
Undergoes proteolytic cleavage.
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For research use only. Not intended for any clinical use.

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