Rabbit Anti-BRAF (Phospho-Thr401) Recombinant Antibody (
4B9) (V2LY-0725-LY612)

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Tested Data
Rabbit Anti-BRAF (Phospho-Thr401) Recombinant Antibody (4B9) (V2LY-0725-LY612) in WB
Rabbit Anti-BRAF (Phospho-Thr401) Recombinant Antibody (4B9) (V2LY-0725-LY612) in WB
Western Blot
Positive WB detected in A549 whole cell lysate(treated with EGF or not)
All lanes Phospho-BRAF antibody at 1.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 KDa
Observed band size: 85 KDa
Rabbit Anti-BRAF (Phospho-Thr401) Recombinant Antibody (4B9) (V2LY-0725-LY612) in IF
Rabbit Anti-BRAF (Phospho-Thr401) Recombinant Antibody (4B9) (V2LY-0725-LY612) in IF
Immunofluorescence staining of HepG2 cells with V2LY-0725-LY612 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Datasheet
SDS
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Basic Information

Host Animal
Rabbit
Clone
4B9
Application
ELISA, WB, IF
Immunogen
A synthesized peptide derived from Human Phospho-BRAF (T401).
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:5,000
IF1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Entrez Gene ID
673
UniProt ID
P15056
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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