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Rabbit Anti-Phospho-CDC25C (Thr48) Recombinant Antibody (D2H3) (CBMAB-CP0243-LY)

The product is antibody recognizes Phospho-CDC25C (Thr48). The antibody D2H3 immunoassay techniques such as: WB,IP,IF (ICC).
See all Phospho-CDC25C (Thr48) antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
D2H3
Antibody Isotype
IgG
Application
WB, IP, IF (ICC)

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr48 of human cdc25C protein.
Specificity
Human, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cell Division Cycle 25C
Introduction
CDC25C (Cell Division Cycle 25C) is a Protein Coding gene. Diseases associated with CDC25C include Plague and Prostate Cancer. Among its related pathways are Gene Expression and Akt Signaling. Gene Ontology (GO) annotations related to this gene include protein kinase binding and WW domain binding. An important paralog of this gene is CDC25B.
Entrez Gene ID
Human995
Monkey714115
UniProt ID
HumanP30307
MonkeyA0A2K5V9P3
Alternative Names
Cell Division Cycle 25C; Protein Phosphatase 1; Regulatory Subunit 60; EC 3.1.3.48; CDC25; Dual Specificity Phosphatase CDC25C; Dual Specificity Phosphatase Cdc25C;
Function
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Biological Process
Cell division Source: UniProtKB-KW
Cell population proliferation Source: ProtInc
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
G2/M transition of mitotic cell cycle Source: UniProtKB
Positive regulation of G2/MI transition of meiotic cell cycle Source: GO_Central
Positive regulation of G2/M transition of mitotic cell cycle Source: GO_Central
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
Regulation of mitotic nuclear division Source: ProtInc
Spermatogenesis Source: Ensembl
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus
PTM
Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 oCcurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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