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Mouse Anti-PLCG1 Recombinant Antibody (2A2) (CBMAB-P2058-YC)

Provided herein is a Mouse monoclonal antibody against Human Phospholipase C gamma 1. The antibody can be used for immunoassay techniques, such as ELISA, IF, PLA, WB.
See all PLCG1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2A2
Antibody Isotype
IgG1, κ
Application
ELISA, IF, PLA, WB

Basic Information

Immunogen
PLCG1 (NP_002651, 1192-1291 aa) partial recombinant protein with GST tag. Immunogen sequence: LKNNYSEDLE LASLLIKIDI FPAKQENGDL SPFSGTSLRE RGSDASGQLF HGRAREGSFE SRYQQPFEDF RISQEHLADH FDSRERRAPR RTRVNGDNRL
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1192-1291

Target

Full Name
phospholipase C, gamma 1
Introduction
PLCG1 (Phospholipase C Gamma 1) is a protein coding gene. Diseases associated with PLCG1 include Paroxysmal Nocturnal Hemoglobinuria and Mycobacterium Kansasii. Among its related pathways are Immune response Fc epsilon RI pathway and EGF/EGFR Signaling Pathway. Gene Ontology annotations related to this gene include calcium ion binding and obsolete signal transducer activity. An important paralog of the gene is PLCG2.
Entrez Gene ID
5335
UniProt ID
P19174
Alternative Names
PLC1; PLC-II; PLCgamma1; NCKAP3; PLC148
Function
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity).
Plays a role in actin reorganization and cell migration (PubMed:17229814).
Biological Process
Calcium-mediated signalingManual Assertion Based On ExperimentIMP:BHF-UCL
Cell migrationManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to epidermal growth factor stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Epidermal growth factor receptor signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Fc-epsilon receptor signaling pathwayTAS:Reactome
Negative regulation of inflammatory response to antigenic stimulusTAS:Reactome
Phosphatidylinositol metabolic processISS:UniProtKB
Phospholipid catabolic processIEA:InterPro
Positive regulation of angiogenesisManual Assertion Based On ExperimentIDA:DFLAT
Positive regulation of blood vessel endothelial cell migrationManual Assertion Based On ExperimentIDA:DFLAT
Positive regulation of endothelial cell apoptotic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of epithelial cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of release of sequestered calcium ion into cytosolManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of vascular endothelial cell proliferationManual Assertion Based On ExperimentIMP:BHF-UCL
T cell receptor signaling pathwayTAS:Reactome
Cellular Location
Cell projection, lamellipodium
Cell projection, ruffle
Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
PTM
Tyrosine phosphorylated in response to signaling via activated FLT3, KIT and PDGFRA (By similarity).
Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated by activated FLT1 and KDR. Tyrosine phosphorylated by activated PDGFRB. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases, in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. May be dephosphorylated by PTPRJ. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells.
Ubiquitinated by CBLB in activated T-cells.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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