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Mouse Anti-POLD4 Recombinant Antibody (2B11) (CBMAB-P2313-YC)

Provided herein is a Mouse monoclonal antibody against Human DNA Polymerase Delta 4, Accessory Subunit. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all POLD4 antibodies
Published Data
Fig.1 Low expression levels of POLD4 in SCLC.
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Fig.2 Effects of POLD4 depletion on in vitro pol δ and NER activities.
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Fig.3 Western blot analysis of POLD4 and a-tubulin in protein extracts from siPOLD4- or siCTRL-treated Calu6 cells.
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Fig.4 DNA repair proteins recruited to regions synthesizing DNA in early mitosis after replicative stress.
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Fig.5 POLD3 promotes faithful chromosome segregation and maintains genome stability.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
2B11
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
POLD4 (AAH01334, 1-34 aa) full length recombinant protein with GST tag. Immunogen sequence: MGRKRLITDS YPVVKRREGP AGHSKGELAP ELGL
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-34

Target

Full Name
polymerase (DNA-directed), delta 4
Introduction
POLD4 is the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein.
Entrez Gene ID
57804
UniProt ID
Q9HCU8
Alternative Names
p12; POLDS
Function
As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity (PubMed:16510448, PubMed:19074196, PubMed:20334433).
Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system (PubMed:24035200).
Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed:24310611).
Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196).
Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed:24022480).
Biological Process
DNA synthesis involved in DNA repairManual Assertion Based On ExperimentIBA:GO_Central
DNA-templated DNA replicationManual Assertion Based On ExperimentIDA:ComplexPortal
Positive regulation of endothelial cell proliferationIEA:Ensembl
Cellular Location
Nucleus
Partially recruited to DNA damage sites within 2 hours following UV irradiation, before degradation.
PTM
Ubiquitinated; undergoes 'Lys-48'-linked ubiquitination in response to UV irradiation, leading to proteasomal degradation (PubMed:17317665, PubMed:16934752, PubMed:23233665, PubMed:23913683).
This modification is partly mediated by RNF8 and by the DCX(DTL) E3 ubiquitin ligase complex (also called CRL4(CDT2)) (PubMed:23233665, PubMed:24022480).
Efficient degradation requires the presence of PCNA and is required for the inhibition of fork progression after DNA damage (PubMed:24022480).
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For research use only. Not intended for any clinical use.

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