Mouse Anti-POLI Recombinant Antibody (1H9) (CBMAB-D2187-YC)

Name: Mouse Anti-POLI Recombinant Antibody (1H9)
SKU: CBMAB-D2187-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1H9

Application

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

DNA Polymerase Iota

Introduction

POLI is an error-prone DNA polymerase involved in DNA repair. POLI promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. POLI inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.

Entrez Gene ID

11201

UniProt ID

Q9UNA4

Alternative Names

eta2; RAD30B; RAD3OB

Function

Error-prone DNA polymerase specifically involved in DNA repair (PubMed:11013228, PubMed:11387224).
Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:11013228, PubMed:11387224, PubMed:14630940, PubMed:15199127).
Favors Hoogsteen base-pairing in the active site (PubMed:15254543).
Inserts the correct base with high-fidelity opposite an adenosine template (PubMed:15254543).
Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine (PubMed:11013228).
May play a role in hypermutation of immunoglobulin genes (PubMed:12410315).
Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (PubMed:11251121, PubMed:14630940).

Biological Process

DNA repairManual Assertion Based On ExperimentTAS:ProtInc
DNA replicationIEA:UniProtKB-KW
Error-prone translesion synthesisTAS:Reactome
Translesion synthesisManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage.

PTM

Monoubiquitinated. Protein monoubiquitination prevents POLI binding to ubiquitin via the ubiquitin-binding motif 1 and ubiquitin-binding motif 2.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-POLI Recombinant Antibody (CBYC-P498) (CAT#: CBMAB-P2332-YC)

Isotype control

For research use only. Not intended for any clinical use.

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