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Mouse Anti-POLI Recombinant Antibody (1H9) (CBMAB-D2187-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DNA Polymerase Iota (POLI). The antibody can be used for immunoassay techniques, such as WB.
See all POLI antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1H9
Antibody Isotype
IgG1
Application
WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Polymerase Iota
Introduction
POLI is an error-prone DNA polymerase involved in DNA repair. POLI promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. POLI inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.
Entrez Gene ID
UniProt ID
Alternative Names
eta2; RAD30B; RAD3OB
Function
Error-prone DNA polymerase specifically involved in DNA repair (PubMed:11013228, PubMed:11387224).
Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:11013228, PubMed:11387224, PubMed:14630940, PubMed:15199127).
Favors Hoogsteen base-pairing in the active site (PubMed:15254543).
Inserts the correct base with high-fidelity opposite an adenosine template (PubMed:15254543).
Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine (PubMed:11013228).
May play a role in hypermutation of immunoglobulin genes (PubMed:12410315).
Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (PubMed:11251121, PubMed:14630940).
Biological Process
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
DNA replicationIEA:UniProtKB-KW
Error-prone translesion synthesisTAS:Reactome
Translesion synthesisManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage.
PTM
Monoubiquitinated. Protein monoubiquitination prevents POLI binding to ubiquitin via the ubiquitin-binding motif 1 and ubiquitin-binding motif 2.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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