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Mouse Anti-PROM1 Antibody (HB#7) (CBMAB-1503CQ)

This product is a mouse antibody that recognizes PROM1. The antibody HB#7 can be used for immunoassay techniques such as: FC, IF, IHC, WB.
See all PROM1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
HB#7
Antibody Isotype
IgG1
Application
FC, IF, IHC, WB

Basic Information

Immunogen
Recombinant chimeric protein (aa 180-380 and aa 612-765)
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
prominin 1
Introduction
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. Diseases associated with PROM1 include Cone-Rod Dystrophy 12 and Macular Dystrophy, Retinal, 2. Among its related pathways are Transcriptional misregulation in cancer and Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways and Lineage-specific Markers.
Entrez Gene ID
8842
UniProt ID
O43490
Alternative Names
PROM1; prominin 1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen
Function
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617).
Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Biological Process
Camera-type eye photoreceptor cell differentiationManual Assertion Based On ExperimentIMP:BHF-UCL
Glomerular parietal epithelial cell differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Photoreceptor cell maintenanceManual Assertion Based On ExperimentIMP:BHF-UCL
Podocyte differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of nephron tubule epithelial cell differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Retina layer formationISS:UniProtKB
Retina morphogenesis in camera-type eyeManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular Location
Apical cell membrane
Cell projection, microvillus membrane
Cell projection, cilium, photoreceptor outer segment
Endoplasmic reticulum
Endoplasmic reticulum-Golgi intermediate compartment
Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
Involvement in disease
Retinitis pigmentosa 41 (RP41):
A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Cone-rod dystrophy 12 (CORD12):
An inherited retinal dystrophy characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa, due to cone photoreceptors degenerating at a higher rate than rod photoreceptors.
Stargardt disease 4 (STGD4):
A common hereditary macular degeneration. It is characterized by decreased central vision, atrophy of the macula and underlying retinal pigment epithelium, and frequent presence of prominent flecks in the posterior pole of the retina.
Retinal macular dystrophy 2 (MCDR2):
A bull's-eye macular dystrophy characterized by bilateral annular atrophy of retinal pigment epithelium at the macula.
Topology
Extracellular: 20-108
Helical: 109-129
Cytoplasmic: 130-157
Helical: 158-178
Extracellular: 179-433
Helical: 434-454
Cytoplasmic: 455-486
Helical: 487-507
Extracellular: 508-792
Helical: 793-813
Cytoplasmic: 814-865
PTM
Isoform 1 and isoform 2 are glycosylated.
Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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