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Mouse Anti-PSTPIP1 Recombinant Antibody (1D5) (CBMAB-P3282-YC)

Provided herein is a Mouse monoclonal antibody against Human Proline-Serine-Threonine Phosphatase Interacting Protein 1. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all PSTPIP1 antibodies
Published Data
Fig.1 Sealing zone dynamics and osteoclast activity in digestion.
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Fig.2 PSTPIP1/2 localization and role in sealing zone dynamics.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1D5
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.2
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
proline-serine-threonine phosphatase interacting protein 1
Introduction
PSTPIP1 is a cytoskeletal protein that is highly expressed in hemopoietic tissues. This protein functions via its interaction with several different proteins involved in cytoskeletal organization and inflammatory processes. It binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, downregulating CD2-triggered adhesion. It binds PEST-type protein tyrosine phosphatases (PTP) and directs them to c-Abl kinase to mediate c-Abl dephosphorylation, thereby, regulating c-Abl activity. It also interacts with pyrin, which is found in association with the cytoskeleton in myeloid/monocytic cells and modulates immunoregulatory functions. Mutations in this gene are associated with PAPA (pyogenic sterile arthritis, pyoderma gangrenosum, and acne) syndrome.
Entrez Gene ID
9051
UniProt ID
O43586
Alternative Names
CD2BP1; CD2BP1L; CD2BP1S; H-PIP; PAPAS; PSTPIP
Function
Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (By similarity).
Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils.
Biological Process
Actin filament polymerizationManual Assertion Based On ExperimentIBA:GO_Central
Cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
EndocytosisIEA:UniProtKB-KW
Inflammatory responseIEA:UniProtKB-KW
Innate immune responseIEA:UniProtKB-KW
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Cytoplasm
Cell membrane
Cell projection, uropodium
Cytoplasm, cytoskeleton
Cytoplasm, perinuclear region
Cell projection, lamellipodium
Cleavage furrow
Mainly cytoplasmic in T-cells (PubMed:9857189).
Colocalizes in cluster with CD2 near the cell surface membrane in activated T-cells (PubMed:9857189).
In monocytes, forms a branched filamentous network in the cytoplasm (PubMed:19584923).
In transfected cells, forms relatively straight filaments radiating out from the nucleus (PubMed:19584923).
Filament formation requires an intact tubulin cytoskeleton (PubMed:19584923).
In migrating neutrophils, colocalizes with PIP5K1C and DNM2 to the trailing edge of the uropod in a actin-dependent manner (PubMed:18480402).
Colocalized with PTPN12 in the cytoplasm and the perinuclear region. During interphase, colocalizes with F-actin in the cortical cytoskeleton, lamellipodia, and stress fibers. In dividing cells, colocalizes with the F-actin rich cytokinetic cleavage furrow. Colocalized with CD2AP and WAS in the actin cytoskeleton within the cytoplasm. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact (By similarity).
Involvement in disease
PAPA syndrome (PAPAS):
Characterized by autosomal dominant inheritance of early-onset, primarily affecting skin and joint tissues. Recurring inflammatory episodes lead to accumulation of sterile, pyogenic, neutrophil-rich material within the affected joints, ultimately resulting in significant destruction.
PTM
Dephosphorylated on Tyr-345 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-345 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV (By similarity).
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For research use only. Not intended for any clinical use.

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