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Rabbit Anti-RIF1 Recombinant Antibody (D2F2M) (CBMAB-CP2269-LY)

The product is antibody recognizes RIF1. The antibody D2F2M immunoassay techniques such as: WB,IP,IF (ICC).
See all RIF1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
D2F2M
Antibody Isotype
IgG
Application
WB, IP, IF (ICC)

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu2110 of human RIF1 protein.
Specificity
Human, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Replication Timing Regulatory Factor 1
Introduction
This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
Entrez Gene ID
Human55183
Monkey694817
UniProt ID
HumanQ5UIP0
MonkeyF6U9Z7
Alternative Names
Replication Timing Regulatory Factor 1; RAP1 Interacting Factor Homolog (Yeast); Rap1-Interacting Factor 1 Homolog;
Function
Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs (PubMed:15342490, PubMed:28241136).
In response to DNA damage, interacts with ATM-phosphorylated TP53BP1 (PubMed:23333306, PubMed:28241136).
Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs (PubMed:28241136).
Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs (PubMed:23333306).
In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase (PubMed:23333306).
Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (By similarity).
Promotes NHEJ of dysfunctional telomeres (By similarity).
Biological Process
Biological Process cell cycleIEA:UniProtKB-KW
Biological Process cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular response to leukemia inhibitory factorIEA:Ensembl
Biological Process DNA repairIEA:UniProtKB-KW
Biological Process negative regulation of double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of gene expression, epigeneticISS:BHF-UCL
Biological Process negative regulation of transcription by RNA polymerase IIISS:BHF-UCL
Biological Process positive regulation of double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of histone H3-K9 methylationISS:BHF-UCL
Biological Process positive regulation of isotype switchingISS:UniProtKB
Biological Process somatic stem cell population maintenanceIEA:Ensembl
Biological Process subtelomeric heterochromatin assemblyISS:BHF-UCL
Biological Process telomere maintenanceISS:BHF-UCL
Biological Process telomere maintenance in response to DNA damageManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular Location
Nucleus
Chromosome
Chromosome, telomere
Cytoplasm, cytoskeleton, spindle
Following interaction with TP53BP1, recruited to sites of DNA damage, such as DSBs (By similarity).
Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs) (PubMed:15342490).
Does not associate with normal telomere structures (PubMed:15342490, PubMed:15583028).
Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase (PubMed:15583028).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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