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Mouse Anti-RNF31 (AA 970-1072) Recombinant Antibody (CBFYH-2735) (CBMAB-H3793-FY)

This product is mouse antibody that recognizes RNF31. The antibody CBFYH-2735 can be used for immunoassay techniques such as: WB, ICC.
See all RNF31 antibodies
Published Data
Fig.1 MFI of RNF31 expression was measured in CD4+ FOXP3+ Treg cells and CD4+ FOXP3- Tconv cells from PBMC of healthy donors.
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Fig.2 RNF31 was knocked down in primary human Treg cells, and the endogenous FOXP3 level was visualized by immunoblotting (IB).
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Fig.3 In vitro expanded human Treg cells were incubated with specific antibodies. Representative confocal images were visualized for endogenous RNF31 (red), and FOXP3 (green) with DAPI used to visualize the nuclei (blue).
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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-2735
Antibody Isotype
IgG1
Application
WB, ICC

Basic Information

Immunogen
E. coli-derived recombinant human HOIP/RNF31, Arg970-Lys1072
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 970-1072

Target

Full Name
Ring Finger Protein 31
Introduction
The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
55072
UniProt ID
Q96EP0
Alternative Names
Ring Finger Protein 31; HOIL-1-Interacting Protein; Zinc In-Between-RING-Finger Ubiquitin-Associated Domain Protein; RING-Type E3 Ubiquitin Transferase RNF31; RING Finger Protein 31; ZIBRA
Function
E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed:17006537, PubMed:19136968, PubMed:20005846, PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:22863777, PubMed:28481331, PubMed:28189684).
LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (PubMed:17006537, PubMed:19136968, PubMed:20005846, PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:22863777, PubMed:28189684).
Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed:21455173, PubMed:28189684).
LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (PubMed:20005846, PubMed:27458237).
The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria (PubMed:28481331, PubMed:34012115).
LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin (PubMed:28481331).
Recruited to the surface of bacteria by RNF213, which initiates the bacterial ubiquitin coat (PubMed:34012115).
The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation (PubMed:28481331, PubMed:34012115).
Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (PubMed:23708998).
RNF31 is required for linear ubiquitination of BCL10, thereby promoting TCR-induced NF-kappa-B activation (PubMed:27777308).
Binds polyubiquitin of different linkage types (PubMed:23708998).
Biological Process
Biological Process CD40 signaling pathwayISS:BHF-UCL
Biological Process defense response to bacteriumManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of necroptotic processIEA:Ensembl
Biological Process positive regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of protein targeting to mitochondrionManual Assertion Based On ExperimentHMP:ParkinsonsUK-UCL
Biological Process positive regulation of xenophagyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein linear polyubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein polyubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process T cell receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
PTM
Autoubiquitinated (PubMed:24726323).
Interaction with OTULIN is required to suppress formation of 'Met-1'-linked polyubiquitin chains and prevent subsequent inactivation of the LUBAC complex (PubMed:24726323).
Cleaved by caspase during apoptosis.
(Microbial infection) Ubiquitinated by S.flexneri E3 ubiquitin-protein ligases IpaH1.4 and IpaH2.5, leading to its degradation by the proteasome, thereby preventing formation of the bacterial ubiquitin coat and activation of innate immunity.
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For research use only. Not intended for any clinical use.

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