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Mouse Anti-SIRT1 Monoclonal Antibody (1E11) (CBMAB-0101-LY)

This product is mouse monoclonal antibody recognizes SIRT1 of human. The antibody 1E11 immunoassay techniques such as: IP, MA, WB.
See all SIRT1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1E11
Antibody Isotype
IgG1
Application
IP, MA, WB

Basic Information

Immunogen
Recombinant peptide (Full Length)
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Sirtuin 1
Introduction
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy.
Entrez Gene ID
UniProt ID
Alternative Names
SIR2L1
Function
NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis and autophagy (PubMed:11672523, PubMed:12006491, PubMed:14976264, PubMed:14980222, PubMed:15126506, PubMed:15152190, PubMed:15205477, PubMed:15469825, PubMed:15692560, PubMed:16079181, PubMed:16166628, PubMed:16892051, PubMed:16998810, PubMed:17283066, PubMed:17290224, PubMed:17334224, PubMed:17505061, PubMed:17612497, PubMed:17620057, PubMed:17936707, PubMed:18203716, PubMed:18296641, PubMed:18662546, PubMed:18687677, PubMed:19188449, PubMed:19220062, PubMed:19364925, PubMed:19690166, PubMed:19934257, PubMed:20097625, PubMed:20100829, PubMed:20203304, PubMed:20375098, PubMed:20620956, PubMed:20670893, PubMed:20817729, PubMed:20955178, PubMed:21149730, PubMed:21245319, PubMed:21471201, PubMed:21504832, PubMed:21555002, PubMed:21698133, PubMed:21701047, PubMed:21775285, PubMed:21807113, PubMed:21841822, PubMed:21890893, PubMed:21947282, PubMed:22274616, PubMed:24415752, PubMed:24824780, PubMed:29765047, PubMed:30409912).
Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression (PubMed:15469825).
Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively (PubMed:15152190, PubMed:14980222, PubMed:14976264).
Serves as a sensor of the cytosolic ratio of NAD+/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction (PubMed:15205477).
Is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT) (By similarity).
Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes (PubMed:18485871).
The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus (PubMed:18485871, PubMed:21504832).
Deacetylates 'Lys-266' of SUV39H1, leading to its activation (PubMed:21504832).
Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1 (PubMed:19188449).
Deacetylates H2A and 'Lys-26' of H1-4 (PubMed:15469825).
Deacetylates 'Lys-16' of histone H4 (in vitro). Involved in NR0B2/SHP corepression function through chromatin remodeling: Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression (PubMed:20375098).
Proposed to contribute to genomic integrity via positive regulation of telomere length; however, reports on localization to pericentromeric heterochromatin are conflicting (By similarity).
Proposed to play a role in constitutive heterochromatin (CH) formation and/or maintenance through regulation of the available pool of nuclear SUV39H1 (PubMed:15469825, PubMed:18004385).
Upon oxidative/metabolic stress decreases SUV39H1 degradation by inhibiting SUV39H1 polyubiquitination by MDM2 (PubMed:18004385, PubMed:21504832).
This increase in SUV39H1 levels enhances SUV39H1 turnover in CH, which in turn seems to accelerate renewal of the heterochromatin which correlates with greater genomic integrity during stress response (PubMed:18004385, PubMed:21504832).
Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce transcription-dependent proapoptotic program and modulate cell senescence (PubMed:11672523, PubMed:12006491).
Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I (By similarity).
Deacetylates MYC, promotes the association of MYC with MAX and decreases MYC stability leading to compromised transformational capability (PubMed:19364925, PubMed:21807113).
Deacetylates FOXO3 in response to oxidative stress thereby increasing its ability to induce cell cycle arrest and resistance to oxidative stress but inhibiting FOXO3-mediated induction of apoptosis transcriptional activity; also leading to FOXO3 ubiquitination and protesomal degradation (PubMed:14980222, PubMed:14976264, PubMed:21841822).
Appears to have a similar effect on MLLT7/FOXO4 in regulation of transcriptional activity and apoptosis (PubMed:15126506).
Deacetylates DNMT1; thereby impairs DNMT1 methyltransferase-independent transcription repressor activity, modulates DNMT1 cell cycle regulatory function and DNMT1-mediated gene silencing (PubMed:21947282).
Deacetylates RELA/NF-kappa-B p65 thereby inhibiting its transactivating potential and augments apoptosis in response to TNF-alpha (PubMed:15152190).
Deacetylates HIF1A, KAT5/TIP60, RB1 and HIC1 (PubMed:17620057, PubMed:17283066, PubMed:20100829, PubMed:20620956).
Deacetylates FOXO1 resulting in its nuclear retention and enhancement of its transcriptional activity leading to increased gluconeogenesis in liver (PubMed:15692560).
Inhibits E2F1 transcriptional activity and apoptotic function, possibly by deacetylation (PubMed:16892051).
Involved in HES1- and HEY2-mediated transcriptional repression (PubMed:12535671).
In cooperation with MYCN seems to be involved in transcriptional repression of DUSP6/MAPK3 leading to MYCN stabilization by phosphorylation at 'Ser-62' (PubMed:21698133).
Deacetylates MEF2D (PubMed:16166628).
Required for antagonist-mediated transcription suppression of AR-dependent genes which may be linked to local deacetylation of histone H3 (PubMed:17505061).
Represses HNF1A-mediated transcription (By similarity).
Required for the repression of ESRRG by CREBZF (PubMed:19690166).
Deacetylates NR1H3 and NR1H2 and deacetylation of NR1H3 at 'Lys-434' positively regulates transcription of NR1H3:RXR target genes, promotes NR1H3 proteosomal degradation and results in cholesterol efflux; a promoter clearing mechanism after reach round of transcription is proposed (PubMed:17936707).
Involved in lipid metabolism: deacetylates LPIN1, thereby inhibiting diacylglycerol synthesis (PubMed:20817729, PubMed:29765047).
Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG which probably involves association with NCOR1 and SMRT/NCOR2 (By similarity).
Deacetylates p300/EP300 and PRMT1 (By similarity).
Deacetylates ACSS2 leading to its activation, and HMGCS1 deacetylation (PubMed:21701047).
Involved in liver and muscle metabolism. Through deacetylation and activation of PPARGC1A is required to activate fatty acid oxidation in skeletal muscle under low-glucose conditions and is involved in glucose homeostasis (PubMed:23142079).
Involved in regulation of PPARA and fatty acid beta-oxidation in liver. Involved in positive regulation of insulin secretion in pancreatic beta cells in response to glucose; the function seems to imply transcriptional repression of UCP2. Proposed to deacetylate IRS2 thereby facilitating its insulin-induced tyrosine phosphorylation. Deacetylates SREBF1 isoform SREBP-1C thereby decreasing its stability and transactivation in lipogenic gene expression (PubMed:17290224, PubMed:20817729).
Involved in DNA damage response by repressing genes which are involved in DNA repair, such as XPC and TP73, deacetylating XRCC6/Ku70, and facilitating recruitment of additional factors to sites of damaged DNA, such as SIRT1-deacetylated NBN can recruit ATM to initiate DNA repair and SIRT1-deacetylated XPA interacts with RPA2 (PubMed:15205477, PubMed:17334224, PubMed:16998810, PubMed:17612497, PubMed:20670893, PubMed:21149730).
Also involved in DNA repair of DNA double-strand breaks by homologous recombination and specifically single-strand annealing independently of XRCC6/Ku70 and NBN (PubMed:15205477, PubMed:17334224, PubMed:20097625).
Promotes DNA double-strand breaks by mediating deacetylation of SIRT6 (PubMed:32538779).
Transcriptional suppression of XPC probably involves an E2F4:RBL2 suppressor complex and protein kinase B (AKT) signaling. Transcriptional suppression of TP73 probably involves E2F4 and PCAF. Deacetylates WRN thereby regulating its helicase and exonuclease activities and regulates WRN nuclear translocation in response to DNA damage (PubMed:18203716).
Deacetylates APEX1 at 'Lys-6' and 'Lys-7' and stimulates cellular AP endonuclease activity by promoting the association of APEX1 to XRCC1 (PubMed:19934257).
Catalyzes deacetylation of ERCC4/XPF, thereby impairing interaction with ERCC1 and nucleotide excision repair (NER) (PubMed:32034146).
Increases p53/TP53-mediated transcription-independent apoptosis by blocking nuclear translocation of cytoplasmic p53/TP53 and probably redirecting it to mitochondria. Deacetylates XRCC6/Ku70 at 'Lys-539' and 'Lys-542' causing it to sequester BAX away from mitochondria thereby inhibiting stress-induced apoptosis. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and MAP1LC3B/ATG8 (PubMed:18296641).
Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation (PubMed:21775285).
Proposed to play role in regulation of STK11/LBK1-dependent AMPK signaling pathways implicated in cellular senescence which seems to involve the regulation of the acetylation status of STK11/LBK1. Can deacetylate STK11/LBK1 and thereby increase its activity, cytoplasmic localization and association with STRAD; however, the relevance of such activity in normal cells is unclear (PubMed:18687677, PubMed:20203304).
In endothelial cells is shown to inhibit STK11/LBK1 activity and to promote its degradation. Deacetylates SMAD7 at 'Lys-64' and 'Lys-70' thereby promoting its degradation. Deacetylates CIITA and augments its MHC class II transactivation and contributes to its stability (PubMed:21890893).
Deacetylates MECOM/EVI1 (PubMed:21555002).
Deacetylates PML at 'Lys-487' and this deacetylation promotes PML control of PER2 nuclear localization (PubMed:22274616).
During the neurogenic transition, represses selective NOTCH1-target genes through histone deacetylation in a BCL6-dependent manner and leading to neuronal differentiation. Regulates the circadian expression of several core clock genes, including ARNTL/BMAL1, RORC, PER2 and CRY1 and plays a critical role in maintaining a controlled rhythmicity in histone acetylation, thereby contributing to circadian chromatin remodeling (PubMed:18662546).
Deacetylates ARNTL/BMAL1 and histones at the circadian gene promoters in order to facilitate repression by inhibitory components of the circadian oscillator (By similarity).
Deacetylates PER2, facilitating its ubiquitination and degradation by the proteosome (By similarity).
Protects cardiomyocytes against palmitate-induced apoptosis (By similarity).
Deacetylates XBP1 isoform 2; deacetylation decreases protein stability of XBP1 isoform 2 and inhibits its transcriptional activity (PubMed:20955178).
Deacetylates PCK1 and directs its activity toward phosphoenolpyruvate production promoting gluconeogenesis (PubMed:30193097).
Involved in the CCAR2-mediated regulation of PCK1 and NR1D1 (PubMed:24415752).
Deacetylates CTNB1 at 'Lys-49' (PubMed:24824780).
In POMC (pro-opiomelanocortin) neurons, required for leptin-induced activation of PI3K signaling (By similarity).
In addition to protein deacetylase activity, also acts as protein-lysine deacylase by mediating protein depropionylation and decrotonylation (PubMed:28497810).
Mediates depropionylation of Osterix (SP7) (By similarity).
Catalyzes decrotonylation of histones; it however does not represent a major histone decrotonylase (PubMed:28497810).
Deacetylates SOX9; promoting SOX9 nuclear localization and transactivation activity (By similarity).
Involved in the regulation of centrosome duplication. Deacetylates CENATAC in G1 phase, allowing for SASS6 accumulation on the centrosome and subsequent procentriole assembly (PubMed:31722219).
Deacetylates NDC80/HEC1 (PubMed:30409912).
Isoform 2
Deacetylates 'Lys-382' of p53/TP53, however with lower activity than isoform 1. In combination, the two isoforms exert an additive effect. Isoform 2 regulates p53/TP53 expression and cellular stress response and is in turn repressed by p53/TP53 presenting a SIRT1 isoform-dependent auto-regulatory loop.
SirtT1 75 kDa fragment
Catalytically inactive 75SirT1 may be involved in regulation of apoptosis. May be involved in protecting chondrocytes from apoptotic death by associating with cytochrome C and interfering with apoptosome assembly.
(Microbial infection) In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. The viral Tat protein inhibits SIRT1 deacetylation activity toward RELA/NF-kappa-B p65, thereby potentiates its transcriptional activity and SIRT1 is proposed to contribute to T-cell hyperactivation during infection.
Biological Process
Biological Process angiogenesisManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process behavioral response to starvationIEA:Ensembl
Biological Process cellular glucose homeostasisISS:UniProtKB
Biological Process cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular response to glucose starvationManual Assertion Based On ExperimentIMP:ComplexPortal
Biological Process cellular response to hydrogen peroxideManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process cellular response to hypoxiaManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process cellular response to ionizing radiationISS:UniProtKB
Biological Process cellular response to leukemia inhibitory factorIEA:Ensembl
Biological Process cellular response to starvationISS:BHF-UCL
Biological Process cellular response to tumor necrosis factorManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular triglyceride homeostasisISS:UniProtKB
Biological Process cholesterol homeostasisISS:UniProtKB
Biological Process chromatin organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process circadian regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process DNA methylation-dependent heterochromatin assemblyManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process DNA synthesis involved in DNA repairISS:UniProtKB
Biological Process energy homeostasisManual Assertion Based On ExperimentIMP:ComplexPortal
Biological Process fatty acid homeostasisISS:UniProtKB
Biological Process heterochromatin assemblyManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process histone deacetylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process histone H3 deacetylationManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediatorManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process leptin-mediated signaling pathwayISS:UniProtKB
Biological Process macrophage differentiationISS:UniProtKB
Biological Process muscle organ developmentIEA:UniProtKB-KW
Biological Process negative regulation of androgen receptor signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of cAMP-dependent protein kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of cell cycleManual Assertion Based On ExperimentIMP:ComplexPortal
Biological Process negative regulation of cell growthManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process negative regulation of cellular response to testosterone stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process negative regulation of cellular senescenceManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of DNA damage response, signal transduction by p53 class mediatorManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process negative regulation of DNA-binding transcription factor activityManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process negative regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of fat cell differentiationISS:BHF-UCL
Biological Process negative regulation of gene expressionManual Assertion Based On ExperimentIMP:CACAO
Biological Process negative regulation of helicase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of histone H3-K14 acetylationManual Assertion Based On ExperimentIMP:CACAO
Biological Process negative regulation of histone H3-K9 trimethylationIEA:Ensembl
Biological Process negative regulation of histone H4-K16 acetylationManual Assertion Based On ExperimentIMP:CACAO
Biological Process negative regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediatorBy SimilarityISS:BHF-UCL
Biological Process negative regulation of neuron deathIEA:Ensembl
Biological Process negative regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process negative regulation of peptidyl-lysine acetylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of prostaglandin biosynthetic processISS:UniProtKB
Biological Process negative regulation of protein acetylationManual Assertion Based On ExperimentIMP:CACAO
Biological Process negative regulation of protein kinase B signalingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of TOR signalingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of transforming growth factor beta receptor signaling pathwayISS:UniProtKB
Biological Process ovulation from ovarian follicleIEA:Ensembl
Biological Process peptidyl-lysine acetylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process peptidyl-lysine deacetylationManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process positive regulation of adaptive immune responseManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of adipose tissue developmentISS:UniProtKB
Biological Process positive regulation of angiogenesisManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process positive regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of blood vessel endothelial cell migrationManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process positive regulation of cAMP-dependent protein kinase activityManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of cellular senescenceManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of cholesterol effluxISS:UniProtKB
Biological Process positive regulation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of DNA repairManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathwayIEA:Ensembl
Biological Process positive regulation of endothelial cell proliferationManual Assertion Based On ExperimentIMP:AgBase
Biological Process positive regulation of gluconeogenesisManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of histone deacetylationManual Assertion Based On ExperimentIMP:ComplexPortal
Biological Process positive regulation of histone H3-K9 methylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of histone methylationManual Assertion Based On ExperimentIMP:ComplexPortal
Biological Process positive regulation of insulin receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of macroautophagyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of macrophage apoptotic processISS:UniProtKB
Biological Process positive regulation of macrophage cytokine productionISS:UniProtKB
Biological Process positive regulation of MHC class II biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of phosphatidylinositol 3-kinase signalingISS:UniProtKB
Biological Process positive regulation of protein phosphorylationISS:UniProtKB
Biological Process positive regulation of smooth muscle cell differentiationIEA:Ensembl
Biological Process positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein deacetylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein depropionylationISS:UniProtKB
Biological Process protein destabilizationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process pyrimidine dimer repair by nucleotide-excision repairManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process rDNA heterochromatin assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of bile acid biosynthetic processISS:UniProtKB
Biological Process regulation of brown fat cell differentiationISS:UniProtKB
Biological Process regulation of cell population proliferationManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process regulation of cellular response to heatTAS:Reactome
Biological Process regulation of centrosome duplicationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of endodeoxyribonuclease activityManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of glucose metabolic processISS:UniProtKB
Biological Process regulation of lipid storageISS:UniProtKB
Biological Process regulation of mitotic cell cycleManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of peroxisome proliferator activated receptor signaling pathwayISS:BHF-UCL
Biological Process regulation of protein serine/threonine kinase activityManual Assertion Based On ExperimentIMP:AgBase
Biological Process regulation of smooth muscle cell apoptotic processISS:UniProtKB
Biological Process regulation of transcription by glucoseManual Assertion Based On ExperimentIMP:ComplexPortal
Biological Process response to hydrogen peroxideManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process response to insulinISS:UniProtKB
Biological Process response to leptinISS:UniProtKB
Biological Process response to oxidative stressManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process single strand break repairManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process spermatogenesisIEA:Ensembl
Biological Process stress-induced premature senescenceManual Assertion Based On ExperimentIMP:CACAO
Biological Process transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process triglyceride mobilizationISS:BHF-UCL
Biological Process UV-damage excision repairManual Assertion Based On ExperimentIMP:CACAO
Biological Process white fat cell differentiationISS:BHF-UCL
Cellular Location
Nucleus, PML body
Cytoplasm
Nucleus
Recruited to the nuclear bodies via its interaction with PML (PubMed:12006491).
Colocalized with APEX1 in the nucleus (PubMed:19934257).
May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane (PubMed:15469825).
Shuttles between nucleus and cytoplasm (By similarity).
Colocalizes in the nucleus with XBP1 isoform 2 (PubMed:20955178).
SirtT1 75 kDa fragment
Cytoplasm
Mitochondrion
PTM
Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53.
Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 activates deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity).
Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545.
Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1).1 Publication
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.
Acetylated at various Lys residues. Deacetylated via an autocatalytic mechanism. Autodeacetylation at Lys-238 promotes its protein deacetylase activity.
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For research use only. Not intended for any clinical use.

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