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Rat Anti-TPPP Recombinant Antibody (CBYJT-4388) (CBMAB-T3882-YJ)

Provided herein is a Rat monoclonal antibody, which binds to TPPP (Tubulin Polymerization Promoting Protein). The antibody can be used for immunoassay techniques, such as ICC, IHC-PS, WB.
See all TPPP antibodies

Summary

Host Animal
Rat
Specificity
Human, Mouse, Monkey
Clone
CBYJT-4388
Antibody Isotype
IgG2a
Application
ICC, IHC-PS, WB

Basic Information

Immunogen
Full lenght recombinant TPPP
Specificity
Human, Mouse, Monkey
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium Azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Tubulin Polymerization Promoting Protein
Introduction
TPPP (Tubulin Polymerization Promoting Protein) is a Protein Coding gene. Diseases related to TPPP include Lymphoid Interstitial Pneumonia and Dementia, Lewy Body. Among its related pathways are Glial Cell Differentiation and Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways. Gene Ontology (GO) annotations related to TPPP include microtubule binding and tubulin binding. An important paralog of TPPP is TPPP3.
Entrez Gene ID
Human11076
Mouse72948
Monkey708834
UniProt ID
HumanO94811
MouseQ7TQD2
MonkeyF6YD17
Alternative Names
Tubulin Polymerization Promoting Protein; Brain Specific Protein P25 Alpha; 25 KDa Brain-Specific Protein; P25-Alpha; TPPP/P25; TPPP1
Function
Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath (PubMed:31522887).
Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath (PubMed:31522887, PubMed:33831707).
Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes (PubMed:31522887).
Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear (PubMed:21995432, PubMed:21316364).
In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network (PubMed:17105200, PubMed:17693641, PubMed:18028908, PubMed:26289831).
Regulates microtubule dynamics by promoting tubulin acetylation: acts by inhibiting the tubulin deacetylase activity of HDAC6 (PubMed:20308065, PubMed:23093407).
Also regulates cell migration: phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility (PubMed:23093407).
Plays a role in cell proliferation by regulating the G1/S-phase transition (PubMed:23355470).
Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 (PubMed:22328514).
Biological Process
Biological Process astral microtubule organization Source:UniProtKB1 Publication
Biological Process cell division Source:UniProtKB-KW
Biological Process microtubule bundle formation Source:UniProtKB2 Publications
Biological Process microtubule nucleation by microtubule organizing center Source:UniProtKB1 Publication
Biological Process microtubule polymerization Source:UniProtKB1 Publication
Biological Process myelin assembly Source:UniProtKB
Biological Process negative regulation of tubulin deacetylation Source:UniProtKB2 Publications
Biological Process oligodendrocyte development Source:UniProtKB
Biological Process oligodendrocyte differentiation Source:UniProtKB
Biological Process positive regulation of myelination Source:UniProtKB
Biological Process positive regulation of protein polymerization Source:HGNC-UCL1 Publication
Biological Process positive regulation of protein-containing complex assembly Source:HGNC-UCL
Biological Process regulation of microtubule cytoskeleton organization Source:UniProtKB1 Publication
Cellular Location
Golgi outpost
Cytoplasm, cytoskeleton, microtubule organizing center
Cytoplasm, cytoskeleton
Nucleus
Cytoplasm, cytoskeleton, spindle
Specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, which shapes dendrite morphology by functioning as sites of acentrosomal microtubule nucleation (By similarity).
Mainly localizes to the cytoskeleton (PubMed:18028908).
Also found in the nucleus; however, nuclear localization is unclear and requires additional evidences (PubMed:18028908).
Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies (PubMed:15590652, PubMed:17027006).
During mitosis, colocalizes with LIMK2 at the mitotic spindle (PubMed:22328514).
PTM
Phosphorylated by LIMK1 on serine residues; phosphorylation may alter the tubulin polymerization activity (PubMed:17693641, PubMed:18028908).
Phosphorylation by LIMK2, but not LIMK1, regulates astral microtubule organization at early stage of mitosis (PubMed:22328514).
Phosphorylation by ROCK1 at Ser-32, Ser-107 and Ser-159 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin, increased cell motility and entry into S-phase (PubMed:23093407, PubMed:23355470).
Phosphorylation by CDK1 inhibits the microtubule polymerizing activity (PubMed:23355470).
Degraded by the proteasome; zinc-binding inhibits degradation by the proteasome.
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For research use only. Not intended for any clinical use.

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