Mouse Anti-TYROBP Recombinant Antibody (CBYCD-526) (CBMAB-D2362-YC)

Mouse

Human

CBYCD-526

IgG1

WB, FC

Human Dap12 synthetic peptide, QGQRSDVYSDLNTQRPYYK, Accession # O43914

Human

IgG1

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

TYROBP Gene(Protein Coding) TYRO Protein Tyrosine Kinase Binding Protein

TYROBP is a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL.

TYRO Protein Tyrosine Kinase Binding Protein; DNAX-Activation Protein 12; Killer-Activating Receptor-Associated Protein; KAR-Associated Protein; DAP12; KARAP; Killer Activating Receptor Associated Protein; TYRO Protein Tyrosine Kinase-Binding Protein; PLOSL;

Adapter protein which non-covalently associates with activating receptors found on the surface of a variety of immune cells to mediate signaling and cell activation following ligand binding by the receptors (PubMed:9490415, PubMed:9655483, PubMed:10604985).
TYROBP is tyrosine-phosphorylated in the ITAM domain following ligand binding by the associated receptors which leads to activation of additional tyrosine kinases and subsequent cell activation (PubMed:9490415).
Also has an inhibitory role in some cells (PubMed:21727189).
Non-covalently associates with activating receptors of the CD300 family to mediate cell activation (PubMed:15557162, PubMed:16920917, PubMed:17928527; PubMed:26221034).
Also mediates cell activation through association with activating receptors of the CD200R family (By similarity).
Required for neutrophil activation mediated by integrin (By similarity).
Required for the activation of myeloid cells mediated by the CLEC5A/MDL1 receptor (PubMed:10449773).
Associates with natural killer (NK) cell receptors such as KIR2DS2 and the KLRD1/KLRC2 heterodimer to mediate NK cell activation (PubMed:9490415, PubMed:9655483, PubMed:23715743).
Also enhances trafficking and cell surface expression of NK cell receptors KIR2DS1, KIR2DS2 and KIR2DS4 and ensures their stability at the cell surface (PubMed:23715743).
Associates with SIRPB1 to mediate activation of myeloid cells such as monocytes and dendritic cells (PubMed:10604985).
Associates with TREM1 to mediate activation of neutrophils and monocytes (PubMed:10799849).
Associates with TREM2 on monocyte-derived dendritic cells to mediate up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:11602640).
Association with TREM2 mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed:18957693).
Stabilizes the TREM2 C-terminal fragment (TREM2-CTF) produced by TREM2 ectodomain shedding which suppresses the release of pro-inflammatory cytokines (PubMed:25957402).
In microglia, required with TREM2 for phagocytosis of apoptotic neurons (By similarity).
Required with ITGAM/CD11B in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
Promotes pro-inflammatory responses in microglia following nerve injury which accelerates degeneration of injured neurons (By similarity).
Positively regulates the expression of the IRAK3/IRAK-M kinase and IL10 production by liver dendritic cells and inhibits their T cell allostimulatory ability (By similarity).
Negatively regulates B cell proliferation (PubMed:21727189).
Required for CSF1-mediated osteoclast cytoskeletal organization (By similarity).
Positively regulates multinucleation during osteoclast development (By similarity).

Biological Process actin cytoskeleton organization Source:UniProtKB
Biological Process apoptotic cell clearance Source:UniProtKB
Biological Process apoptotic signaling pathway Source:ARUK-UCL
Biological Process cellular defense response Source:ProtInc1 Publication
Biological Process forebrain development Source:ARUK-UCL
Biological Process intracellular signal transduction Source:ProtInc1 Publication
Biological Process microglial cell activation involved in immune response Source:UniProtKB
Biological Process myeloid leukocyte activation Source:UniProtKB1 Publication
Biological Process negative regulation of B cell proliferation Source:UniProtKB1 Publication
Biological Process negative regulation of interleukin-10 production Source:ARUK-UCL
Biological Process negative regulation of long-term synaptic potentiation Source:ARUK-UCL
Biological Process negative regulation of transforming growth factor beta1 production Source:ARUK-UCL
Biological Process neutrophil activation involved in immune response Source:GO_Central1 Publication
Biological Process osteoclast differentiation Source:MGI1 Publication
Biological Process positive regulation of gene expression Source:ARUK-UCL
Biological Process positive regulation of hippocampal neuron apoptotic process Source:ARUK-UCL
Biological Process positive regulation of interleukin-1 beta production Source:ARUK-UCL
Biological Process positive regulation of interleukin-6 production Source:ARUK-UCL
Biological Process positive regulation of macrophage fusion Source:UniProtKB1 Publication
Biological Process positive regulation of microglial cell mediated cytotoxicity Source:UniProtKB
Biological Process positive regulation of natural killer cell activation Source:GO_Central1 Publication
Biological Process positive regulation of neuron death Source:ARUK-UCL
Biological Process positive regulation of osteoclast development Source:UniProtKB
Biological Process positive regulation of protein localization to cell surface Source:UniProtKB1 Publication
Biological Process positive regulation of receptor localization to synapse Source:ARUK-UCL
Biological Process positive regulation of superoxide anion generation Source:ARUK-UCL
Biological Process positive regulation of tumor necrosis factor production Source:ARUK-UCL
Biological Process protein stabilization Source:UniProtKB1 Publication
Biological Process response to axon injury Source:ARUK-UCL
Biological Process signal transduction Source:ProtInc1 Publication
Biological Process stimulatory C-type lectin receptor signaling pathway Source:UniProtKB1 Publication
Biological Process stimulatory killer cell immunoglobulin-like receptor signaling pathway Source:UniProtKB1 Publication

Cell membrane

Polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy 1 (PLOSL1):
A recessively inherited disease characterized by presenile dementia along with large-scale destruction of cancellous bones. Initial symptoms, starting in the twenties, are pain and swelling resulting from cysts in the wrists and ankles. Extremity bone fractures could occur with minor trauma. At around 30 years of age, patients gradually develop neuropsychiatric symptoms, including epileptic seizures, agnosia, apraxia, speech disorder, memory disturbance, euphoria, and loss of social inhibitions. The disorder usually leads to death in the fifth decade of life.

Extracellular: 22-40
Helical: 41-61
Cytoplasmic: 62-113

Following ligand binding by associated receptors, tyrosine phosphorylated in the ITAM domain which leads to activation of additional tyrosine kinases and subsequent cell activation.