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Mouse Anti-USP36 (aa 589-972) Recombinant Antibody (3B9) (CBMAB-U0067-CQ)

This product is a mouse antibody that recognizes USP36 (aa 589-972). The antibody 3B9 can be used for immunoassay techniques such as: IF, WB.
See all USP36 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3B9
Antibody Isotype
IgG1
Application
IF, WB

Basic Information

Immunogen
Recombinant protein fragment between amino acids 589-972 of human USP36 produced in E.coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/mL
Purity
>95% as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 589-972

Target

Full Name
ubiquitin specific peptidase 36
Introduction
This gene encodes a member of the peptidase C19 or ubiquitin-specific protease family of cysteine proteases. Members of this family remove ubiquitin molecules from polyubiquitinated proteins.
Entrez Gene ID
57602
UniProt ID
Q9P275
Alternative Names
USP36; ubiquitin specific peptidase 36; ubiquitin specific protease 36; ubiquitin carboxyl-terminal hydrolase 36; FLJ12851; KIAA1453; deubiquitinating enzyme 1; ubiquitin thioesterase 36; deubiquitinating enzyme 36; ubiquitin thiolesterase 36; ubiquitin-specific-processing protease 36; DUB1
Function
Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability. Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability (PubMed:29273634, PubMed:19208757, PubMed:22902402).
Functions as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) (PubMed:29274341).
Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm (PubMed:25775507).
Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions (PubMed:27445338).
Deubiquitinates SOD2, regulates SOD2 protein stability (PubMed:21268071).
Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins (PubMed:22622177).
Biological Process
Biological Process histone deubiquitination Source:UniProtKB1 Publication
Biological Process negative regulation of macroautophagy Source:UniProtKB1 Publication
Biological Process nucleolus organization Source:UniProtKB
Biological Process positive regulation of protein targeting to mitochondrion Source:ParkinsonsUK-UCL1 Publication
Biological Process protein deubiquitination Source:UniProtKB2 Publications
Biological Process protein stabilization Source:UniProtKB
Biological Process regulation of apoptotic process Source:GO_Central1 Publication
Biological Process regulation of autophagy of mitochondrion Source:ParkinsonsUK-UCL1 Publication
Biological Process regulation of protein stability Source:UniProtKB1 Publication
Biological Process regulation of rRNA processing Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro
Cellular Location
Nucleus, nucleolus
Cytoplasm
PTM
Polyubiquitinated by NEDD4L, no effect on USP36 protein levels. Both proteins interact with and regulate each other's ubiquitination levels.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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