Mouse Anti-USP36 (aa 589-972) Recombinant Antibody (3B9) (CBMAB-U0067-CQ)

Name: Mouse Anti-USP36 (aa 589-972) Recombinant Antibody (3B9)
SKU: CBMAB-U0067-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

3B9

Application

IF, WB

Immunogen

Recombinant protein fragment between amino acids 589-972 of human USP36 produced in E.coli

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/mL

Purity

>95% as determined by SDS-PAGE analysis

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 589-972

More Infomation

Target

Full Name

ubiquitin specific peptidase 36

Introduction

This gene encodes a member of the peptidase C19 or ubiquitin-specific protease family of cysteine proteases. Members of this family remove ubiquitin molecules from polyubiquitinated proteins.

Entrez Gene ID

57602

UniProt ID

Q9P275

Alternative Names

USP36; ubiquitin specific peptidase 36; ubiquitin specific protease 36; ubiquitin carboxyl-terminal hydrolase 36; FLJ12851; KIAA1453; deubiquitinating enzyme 1; ubiquitin thioesterase 36; deubiquitinating enzyme 36; ubiquitin thiolesterase 36; ubiquitin-specific-processing protease 36; DUB1

Function

Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability. Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability (PubMed:29273634, PubMed:19208757, PubMed:22902402).
Functions as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) (PubMed:29274341).
Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm (PubMed:25775507).
Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions (PubMed:27445338).
Deubiquitinates SOD2, regulates SOD2 protein stability (PubMed:21268071).
Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins (PubMed:22622177).

Biological Process

Biological Process histone deubiquitination Source:UniProtKB1 Publication
Biological Process negative regulation of macroautophagy Source:UniProtKB1 Publication
Biological Process nucleolus organization Source:UniProtKB
Biological Process positive regulation of protein targeting to mitochondrion Source:ParkinsonsUK-UCL1 Publication
Biological Process protein deubiquitination Source:UniProtKB2 Publications
Biological Process protein stabilization Source:UniProtKB
Biological Process regulation of apoptotic process Source:GO_Central1 Publication
Biological Process regulation of autophagy of mitochondrion Source:ParkinsonsUK-UCL1 Publication
Biological Process regulation of protein stability Source:UniProtKB1 Publication
Biological Process regulation of rRNA processing Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro

Cellular Location

Nucleus, nucleolus
Cytoplasm

PTM

Polyubiquitinated by NEDD4L, no effect on USP36 protein levels. Both proteins interact with and regulate each other's ubiquitination levels.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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