Rabbit Anti-VDAC1 Recombinant Antibody (
3A1) (V2LY-0725-LY911)

Name: Rabbit Anti-VDAC1 Recombinant Antibody (3A1)
SKU: V2LY-0725-LY911
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-VDAC1 Recombinant Antibody (3A1) (V2LY-0725-LY911) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, LO2 whole cell lysate, Raji whole cell lysate, Rat heart tissue, Mouse brain tissue
All lanes: VDAC1 antibody at 0.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 KDa
Observed band size: 31 KDa

Rabbit Anti-VDAC1 Recombinant Antibody (3A1) (V2LY-0725-LY911) in IHC

IHC image of V2LY-0725-LY911 diluted at 1:73.125 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-VDAC1 Recombinant Antibody (3A1) (V2LY-0725-LY911) in IHC

IHC image of V2LY-0725-LY911 diluted at 1:73.125 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

3A1

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human VDAC1.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

voltage dependent anion channel 1

Entrez Gene ID

7416

UniProt ID

P21796

Function

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959).
Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterol cholesterol (PubMed:31015432).
In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033).
May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756).
May mediate ATP export from cells (PubMed:30061676).

Biological Process

Biological Process anion transport Source:UniProtKB1 Publication
Biological Process apoptotic process Source:UniProtKB1 Publication
Biological Process behavioral fear response Source:Ensembl
Biological Process epithelial cell differentiation Source:UniProtKB1 Publication
Biological Process learning Source:Ensembl
Biological Process negative regulation of apoptotic process Source:UniProtKB1 Publication
Biological Process negative regulation of calcium import into the mitochondrion Source:UniProtKB1 Publication
Biological Process negative regulation of reactive oxygen species metabolic process Source:Ensembl
Biological Process neuron-neuron synaptic transmission Source:Ensembl
Biological Process positive regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization Source:UniProtKB1 Publication
Biological Process pyruvate metabolic process Source:Reactome
Biological Process regulation of autophagy of mitochondrion Source:ParkinsonsUK-UCL1 Publication

Cellular Location

Mitochondrion outer membrane
Cell membrane
Membrane raft

PTM

Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.
Ubiquitinated (PubMed:25621951, PubMed:32047033).
Undergoes monoubiquitination and polyubiquitination by PRKN; monoubiquitination at Lys-274 inhibits apoptosis, whereas polyubiquitination leads to its degradation and promotes mitophagy (PubMed:25621951, PubMed:32047033).
Deubiquitinated by USP30 (PubMed:25621951).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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