Mouse Anti-YBX1 Recombinant Antibody (21A3) (CBMAB-Y0015-LY)

YBX1

This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. Regulates the transcription of numerous genes.

YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1

DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation (PubMed:8188694, PubMed:10817758, PubMed:11698476, PubMed:14718551, PubMed:18809583, PubMed:31358969).
Predominantly acts as a RNA-binding protein: binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C) (PubMed:19561594, PubMed:31358969).
Promotes mRNA stabilization: acts by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay (PubMed:10817758, PubMed:11698476, PubMed:31358969).
Component of the CRD-mediated complex that promotes MYC mRNA stability (PubMed:19029303).
Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity).
Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs (PubMed:27559612, PubMed:29073095).
Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C)-containing RNAs (PubMed:28341602, PubMed:29073095).
Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence: acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs (PubMed:29712925).
Also involved in pre-mRNA alternative splicing regulation: binds to splice sites in pre-mRNA and regulates splice site selection (PubMed:12604611).
Also able to bind DNA: regulates transcription of the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7' (PubMed:18809583).
Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes (PubMed:8188694, PubMed:18809583).
Promotes separation of DNA strands that contain mismatches or are modified by cisplatin (PubMed:14718551).
Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA, suggesting a role in DNA repair (PubMed:14718551).
The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation (PubMed:19483673).

Cellular response to interleukin-7IEA:Ensembl
CRD-mediated mRNA stabilizationManual Assertion Based On ExperimentIDA:ComplexPortal
Embryonic morphogenesisISS:UniProtKB
Epidermis developmentISS:UniProtKB
In utero embryonic developmentIEA:Ensembl
miRNA transportManual Assertion Based On ExperimentIDA:UniProtKB
mRNA processingIEA:UniProtKB-KW
mRNA stabilizationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cellular senescenceISS:UniProtKB
Negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decayManual Assertion Based On ExperimentIDA:ComplexPortal
Negative regulation of striated muscle cell differentiationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Negative regulation of translationISS:UniProtKB
Positive regulation of cell divisionIEA:UniProtKB-KW
Positive regulation of cytoplasmic translationManual Assertion Based On ExperimentIDA:ComplexPortal
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Protein localization to cytoplasmic stress granuleManual Assertion Based On ExperimentIMP:AgBase
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
RNA splicingIEA:UniProtKB-KW
RNA transportManual Assertion Based On ExperimentIMP:UniProtKB
tRNA transportManual Assertion Based On ExperimentIMP:UniProtKB

Cytoplasm
Nucleus
Cytoplasmic granule
Secreted
Secreted, extracellular exosome
Predominantly cytoplasmic in proliferating cells (PubMed:12604611).
Cytotoxic stress and DNA damage enhance translocation to the nucleus (PubMed:14718551).
Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:25229427).
Shuttles between nucleus and cytoplasm (PubMed:25229427).
Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress (PubMed:18335541).
Secreted by mesangial and monocytic cells after inflammatory challenges (PubMed:19483673).

Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcactivational ability.
In the absence of phosphorylation the protein is retained in the cytoplasm.
Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus (By similarity).