CCL5 Antibodies

Background

CCL5 is a small molecule chemokine protein mainly secreted by T lymphocytes and platelets. It mediates the migration and activation of immune cells by specifically binding to chemokine receptors (such as CCR5) on the cell surface. This gene plays a core role in the regulation of inflammatory responses, viral infections and the tumor microenvironment, and is particularly crucial in the process of recruiting white blood cells to the infection site. CCL5 was first identified in 1993, and its tertiary structure was resolved by nuclear magnetic resonance technology in 1997, revealing a specific binding mode for interaction with G protein-coupled receptors. As a key molecule in the immune regulatory network, the research on CCL5 has greatly promoted the development of treatment strategies for inflammatory diseases and targeted drug development.

Structure Function Application Advantage Our Products

Structure of CCL5

CCL5 is a relatively small protein with a molecular weight of approximately 7.8 kDa. This molecular weight may vary slightly due to minor differences in amino acid sequences among species.

Species	Human	Mouse	Rat	Rhesus monkey	Rabbit
Molecular Weight (kDa)	7.8	7.9	7.8	7.8	7.7
Primary Structural Differences	Conservative sequence, highly similar to primates	There are several amino acid replacements	With the human homology is higher	Almost identical to the human sequence	Slightly different, but similar in function

The CCL5 protein is typically composed of 68 amino acids and forms a typical chemokine folding structure. Its primary structure consists of a conformed three-strand β -fold and an α -helix at the C-terminal, stabilizing the spatial conformation through disulfide bonds (typically Cys10-Cys34, Cys11-Cys50, and Cys34-Cys50). The core region of this protein has a typical "Gly-Pro" motif, which is responsible for binding to the receptor CCR5. Amino acids at positions 1 to 8 of the N-terminal play a crucial role in receptor activation and signal transduction, while amino acids at positions 44 to 55 are involved in heparin binding and oligomerization processes.

Fig. 1 Receptors related of the chemokine CCL5.¹

Key structural properties of CCL5:

Conservative chemokine folding structure
N-terminal domains mediate receptor recognition and activation
Two pairs of conserved disulfide bonds (Cys10-Cys34, Cys11-Cys50) maintain structural stability
Basic amino acid enrichment region involved in glycosaminoglycans (GAG) combined with sugar and oligomerization

Functions of CCL5

The main function of CCL5 is to mediate the chemotaxis and activation of immune cells. In addition, it is also involved in a variety of pathophysiological processes, including inflammatory regulation, antiviral responses, and the regulation of the tumor immune microenvironment.

Function	Description
Chemotaxis of immune cells	By binding to receptors such as CCR5, it recruits T cells, monocytes, etc. to the site of inflammation or infection, mediating specific immune responses.
Inflammatory regulation	In chronic inflammation and autoimmune disease high expression, promote the leukocyte infiltration and amplify inflammatory signals.
Antiviral response	Early in infection were induced to express, enhance the body's antiviral immunity, such as impact of the virus into and copy in HIV infection.
Tumor immune regulation	Play a dual role in tumor microenvironment: can recruit antitumor immune cells, may also promote tumor growth and metastasis, depending on the context.
Tissue repair and fibrosis	Involved in wound healing process, but the continuous high expression could also lead to fibrosis pathological changes, such as pulmonary fibrosis and liver fibrosis.

The binding of CCL5 to its receptors (such as CCR5) can activate G protein-dependent and independent signaling pathways, including MAPK/ERK and JAK/STAT pathways, triggering intracellular calcium flow and cytoskeletal recombination, thereby driving directed cell migration. Its function is concentration-dependent. At low concentrations, it mainly chemotaxis T cells and dendritic cells, while at high concentrations, it can activate granulocytes and promote degranulation.

Applications of CCL5 and CCL5 Antibody in Literature

1. Barczak, Katarzyna, et al. "CCL5's role in periodontal disease: a narrative review." International Journal of Molecular Sciences 24.24 (2023): 17332. https://doi.org/10.3390/ijms242417332

This article summarizes the role of CCL5 in the occurrence and development of periodontitis, explores its association with disease risk factors and pathological processes, and emphasizes the potential of developing therapeutic drugs targeting CCL5 and its receptors.

2. Song, Lei, et al. "Integrin β8 facilitates macrophage infiltration and polarization by regulating CCL5 to Promote LUAD progression." Advanced Science 12.2 (2025): 2406865. https://doi.org/10.1002/advs.202406865

Research has found that in lung adenocarcinoma, ITGβ8 induces M2 polarization of macrophages through the CCL5 chemokine and the PI3K/AKT/IRF9 pathway, thereby promoting tumor progression, and has revealed the key mediating role of CCL5 in this regulatory circuit.

3. Ma, Weijiang, et al. "The intricate role of CCL5/CCR5 axis in Alzheimer disease." Journal of Neuropathology & Experimental Neurology 82.11 (2023): 894-900. https://doi.org/10.1093/jnen/nlad071

This review focuses on CCL5 and its receptor CCR5, exploring the complex role of this signaling axis in Alzheimer's disease (AD). Although amyloid protein and tau are traditional markers of AD, neuroinflammation is increasingly regarded as a key driver. The article provides a detailed summary of the dual roles of CCL5/CCR5 in AD: they may be involved in mediating neuroinflammation and cognitive dysfunction, but their specific mechanisms and impacts remain controversial. In addition, the review also covers the physiological structure, function of this axis and the research progress as a potential therapeutic target.

4. Zou, Chenghui, et al. "Association between CCL5, CCL11, and CCL17 polymorphisms and atopic dermatitis risk: A systematic review and meta-analysis." Medicine 103.8 (2024): e36897. https://doi.org/10.1097/MD.0000000000036897

This meta-analysis found that CCL5 gene polymorphism is significantly associated with the risk of atopic dermatitis (AD) : the A allele and AG genotype at the -403G/A locus of CCL5, as well as the G allele and GG+GC genotype at the -28C/G locus, all increase the risk of AD development.

5. Zhang, n-Feng, et al. "The regulatory network of the chemokine CCL5 in colorectal cancer." Annals of medicine 55.1 (2023): 2205168. https://doi.org/10.1080/07853890.2023.2205168

The article indicates that CCL5 plays a complex dual role in colorectal cancer: it can not only directly promote tumor metastasis but also reshape the tumor microenvironment by recruiting various immune cells. However, its ultimate effect remains controversial, and the specific mechanism needs to be further clarified.

Creative Biolabs: CCL5 Antibodies for Research

Creative Biolabs specializes in the production of high-quality CCL5 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

Custom CCL5 Antibody Development: Tailor-made solutions to meet specific research requirements.
Bulk Production: Large-scale antibody manufacturing for industry partners.
Technical Support: Expert consultation for protocol optimization and troubleshooting.
Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our CCL5 antibodies, custom preparations, or technical support, contact us at email.

Reference

Zhang, n-Feng, et al. "The regulatory network of the chemokine CCL5 in colorectal cancer." Annals of medicine 55.1 (2023): 2205168. https://doi.org/10.1080/07853890.2023.2205168