CEBPB Antibodies

Structure of CEBPB

CEBPB (CCAAT enhancer-binding protein β) is a basic leucine zipper transcription factor with a molecular weight of approximately 35-45 kDa. Its specific weight varies among different functional subtypes (such as LAP and LIP), mainly due to post-translational modifications and variable translation start sites.

This protein is composed of approximately 345 amino acids, and its primary structure forms a compact basic leucine zipper (bZIP) domain. This domain contains a DNA-binding region rich in basic amino acids and a periodic leucine repeat sequence (zipper region), which stabilizes its binding to DNA by forming homologous or heterodimers. Its tertiary structure is mainly α -helical. When the zipper regions of the two monomers entangle and combine like "zippers", the adjacent alkaline regions can precisely be embedded in the large groove of the CCAAT box of the target gene promoter, thereby initiating or regulating gene transcription.

Fig. 1 Structures of human CEBPB as predicted by AlphaFold.¹

Key structural properties of CEBPB:

• Basic leucine zipper (bZIP) domain is its core functional unit
• Highly conservative alkaline region is responsible for the specific recognition and combining CCAAT on DNA sequence
• Periodically arranged leucine residues form a "zipper" interface, mediating protein dimerization
• The N-terminal transcriptional activation domain contains multiple phosphorylation sites

Functions of CEBPB

CEBPB is a key transcription factor whose main function is to regulate gene expression to coordinate cell differentiation and stress response. In addition, it is also widely involved in various physiological and pathological processes such as immune regulation, metabolic homeostasis and cell cycle control.

CEBPB binds to the CCAAT sequence by forming homologous or heterodimers, and its transcriptional activity is dynamically regulated by post-translational modifications such as phosphorylation and acetylation, thereby precisely regulating the spatiotemporal expression of downstream target genes based on different signal inputs.

Applications of CEBPB and CEBPB Antibody in Literature

1. Yang, Yongchang, et al. "The CEBPB+ glioblastoma subcluster specifically drives the formation of M2 tumor-associated macrophages to promote malignancy growth." Theranostics 14.10 (2024): 4107. https://doi.org/10.7150/thno.93473

This study, combining single-cell and spatial transcriptomics, discovered that the glioblastoma subpopulation GBM6 regulates the malignant progression of tumors through the CEBPB network. The mechanism involves McP1-mediated recruitment of M2-type macrophages and SPP1-Integrin αvβ1-Akt pathway regulation of polarization, providing new ideas for targeted therapy.

2. Wang, Dan, et al. "Colorectal cancer cell-derived CCL20 recruits regulatory T cells to promote chemoresistance via FOXO1/CEBPB/NF-κB signaling." Journal for immunotherapy of cancer 7.1 (2019): 215. https://doi.org/10.1186/s40425-019-0701-2

This study found that colorectal cancer cells mediate chemotherapy resistance to 5-FU by activating the FOXO1/CEBPB/NF-κB axis, upregulating CCL20 expression and recruiting Treg cells. Blocking this pathway can inhibit tumor progression and restore drug sensitivity.

3. Zhou, Zili, et al. "Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis." Journal of translational medicine 20.1 (2022): 262. https://doi.org/10.1186/s12967-022-03467-8

Research has found that stress-induced epinephrine activates CEBPB through β 2-epinephrine receptors, upregates TRIM2 expression, and subsequently ubiquitinates and degrades p53, ultimately promoting the proliferation, metastasis and generation of tumor stem cells of colorectal cancer.

4. Zhou, Xiaohai, et al. "IGF2 deficiency promotes liver aging through mitochondrial dysfunction and upregulated CEBPB signaling in D-galactose-induced aging mice." Molecular Medicine 29.1 (2023): 161. https://doi.org/10.1186/s10020-023-00752-0

This study reveals that liver aging is accompanied by a decrease in IGF2 levels. IGF2 deficiency can promote cellular senescence phenotypes and SASP by inducing mitochondrial dysfunction and upregulating CEBPB signaling, and accelerate the process of liver senescence, suggesting that IGF2 can be used as an intervention target.

5. Greene, Lloyd A., et al. "Targeting transcription factors ATF5, CEBPB and CEBPD with cell-penetrating peptides to treat brain and other cancers." Cells 12.4 (2023): 581. https://doi.org/10.3390/cells12040581

This article describes a rare case of primary multiple pleomorphic rhabdomyosarcoma in a dog's heart, where myoglobin antibody was used as an immunohistochemical marker to confirm the tumor's myogenic origin, demonstrating its diagnostic significance in differentiating cardiac neoplasms.

Creative Biolabs: CEBPB Antibodies for Research

Creative Biolabs specializes in the production of high-quality CEBPB antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom CEBPB Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our CEBPB antibodies, custom preparations, or technical support, contact us at email.