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NMI

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]
Full Name
N-Myc And STAT Interactor
Function
Acts as a signaling pathway regulator involved in innate immune system response (PubMed:9989503, PubMed:26342464, PubMed:29038465, PubMed:29350881).
In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis (PubMed:9989503, PubMed:29377960).
Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription (PubMed:9989503).
In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 (PubMed:10779520, PubMed:10950963).
In complex with IFI35, inhibits virus-triggered type I IFN-beta production when ubiquitinated by ubiquitin-protein ligase TRIM21 (PubMed:26342464).
In complex with IFI35, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed:29350881).
Negatively regulates virus-triggered type I interferon/IFN production by inducing proteosome-dependent degradation of IRF7, a transcriptional regulator of type I IFN, thereby interfering with cellular antiviral responses (By similarity).
Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation, when actively released by macrophage to the extracellular space during cell injury or pathogen invasion (PubMed:29038465).
Macrophage-secreted NMI activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 binding and activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines (PubMed:29038465).
Biological Process
Interferon-gamma-mediated signaling pathwayIEA:InterPro
Macrophage activation involved in immune responseManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of innate immune responseManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of interferon-alpha productionIEA:Ensembl
Negative regulation of interferon-beta productionIEA:Ensembl
Negative regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of inflammatory responseISS:UniProtKB
Positive regulation of innate immune responseISS:UniProtKB
Positive regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein K48-linked ubiquitinationIEA:Ensembl
Receptor signaling pathway via JAK-STATManual Assertion Based On ExperimentTAS:UniProtKB
Response to virusISS:UniProtKB
Toll-like receptor 4 signaling pathwayISS:UniProtKB
Transcription by RNA polymerase IIIEA:InterPro
Cellular Location
Cytoplasm
Nucleus
Secreted
Cytoplasmic NMI localizes in punctate granular structures (PubMed:9781816, PubMed:10950963).
Nuclear localization increased following IFN-alpha treatment (PubMed:9781816, PubMed:10950963).
Extracelullar following secretion by macrophage (PubMed:29038465).
PTM
Ubiquitinated. 'Lys-63'-linked ubiquitination by TRIM21 promotes interaction with IFI35 and inhibits virus-triggered type I IFN-beta production.

Anti-NMI antibodies

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Target: NMI
Host: Mouse
Specificity: Human
Clone: D-10
Application*: WB, IP, IF, P, E
Target: NMI
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 9B8
Application*: E, IH, WB
Target: NMI
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 3C6
Application*: WB
Target: NMI
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: 2C1
Application*: WB
Target: NMI
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 1A8
Application*: WB
Target: NMI
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: CBWJN-0242
Application*: E, WB
Target: NMI
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 9E8
Application*: E, P, WB
Target: NMI
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 10E5
Application*: E, WB
Target: NMI
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 9D8
Application*: E, IH, WB
Target: NMI
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Mouse, Rat
Clone: 2F3
Application*: IHC-P, WB
Target: NMI
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 1E6
Application*: WB, E
Target: NMI
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: 1C1
Application*: IP, M
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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