SLIRP Antibodies

Structure of SLIRP

SLIRP is a relatively small RNA-binding protein with a molecular weight of approximately 18.5 kDa. This molecular weight varies slightly among different species or different isoforms of the same species, mainly due to the minor changes in amino acid sequence caused by its alternative splicing.

The SLIRP protein consists of approximately 160 amino acids and forms its functional core through its RNA recognition motif (RRM). The spatial conformation of this protein enables it to specifically recognize and bind to the specific stem-loop structure of the nuclear receptor co-activator SRA RNA. The α helices and β sheets in its structure jointly maintain a binding interface, which is crucial for its function in regulating gene transcription and participating in mitochondrial RNA metabolism.

Fig. 1 Structure of mitoribosome with LRPPRC–SLIRP bound to mRNA.¹

Key structural properties of SLIRP:

• Contains a classic RNA recognition motif (RRM)
• Core structure specificity of recognition and combination of SRA RNA stem loop structure
• Have approved a signal and mitochondria double location information of target sequence

Functions of SLIRP

The main function of the SLIRP gene is to act as a dual regulator for transcription and mitochondrial function. The specific physiological processes it is involved in are as follows:

The mechanism of SLIRP highlights its pivotal role as a key node linking nuclear gene expression with mitochondrial energy metabolism. This cross-organelle coordination function is of utmost importance for maintaining cellular homeostasis.

Applications of SLIRP and SLIRP Antibody in Literature

1. Singh, Vivek, et al. "Structural basis of LRPPRC–SLIRP-dependent translation by the mitoribosome." Nature structural & molecular biology 31.12 (2024): 1838-1847. https://doi.org/10.1038/s41594-024-01365-9

The mitochondrial factor LRPPRC forms a complex with SLIRP. Through structural analysis, it was found that this complex can bind to mRNA and assist in its delivery to the mitochondrial ribosomes. Studies have shown that this complex can regulate the translation efficiency of different mRNAs, especially affecting the synthesis of cytochrome c oxidase subunits, and is an important regulator of mitochondrial gene expression.

2. Colley, Shane M., et al. "Loss of the nuclear receptor corepressor SLIRP compromises male fertility." PLoS One 8.8 (2013): e70700. https://doi.org/10.1371/journal.pone.0070700

This study reveals that the SLIRP protein not only inhibits the activity of nuclear receptors, but also is abnormally distributed in the testes. In genetically knockout mice, the absence of SLIRP leads to a decline in male fertility, impaired sperm motility and mitochondrial structure, thereby causing asthenospermia.

3. De Silva, Dinuka, et al. "Interaction between androgen receptor and coregulator SLIRP is regulated by Ack1 tyrosine kinase and androgen." Scientific Reports 9.1 (2019): 18637. https://doi.org/10.1038/s41598-019-55057-2

This study reveals that SLIRP, as a co-regulator of the androgen receptor (AR), binds to target genes in the absence of androgens. The Ack1 kinase and androgen signaling can disrupt the binding of AR-SLIRP, affecting the transcription of related genes, suggesting its role in castration-resistant prostate cancer.

4. Lagouge, Marie, et al. "SLIRP regulates the rate of mitochondrial protein synthesis and protects LRPPRC from degradation." PLoS genetics 11.8 (2015): e1005423. https://doi.org/10.1371/journal.pgen.1005423

This study reveals that SLIRP mainly stabilizes the LRPPRC protein in the LRPPRC-SLIRP complex and is responsible for mediating the binding of mitochondrial mRNA to ribosomes, thereby regulating translation efficiency. The study found that mitochondrial mRNA exhibits significant redundancy under normal physiological conditions.

5. Baleva, Mariia V., et al. "Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells." International Journal of Molecular Sciences 25.1 (2023): 93. https://doi.org/10.3390/ijms25010093

This study reveals that the SLIRP protein specifically binds to the small subunit of mitochondrial ribosomes and regulates translation initiation. Its absence interferes with mitochondrial translation, leading to dysfunction of complexes I and IV, but does not affect complexes III and V.

Creative Biolabs: SLIRP Antibodies for Research

Creative Biolabs specializes in the production of high-quality SLIRP antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom SLIRP Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our SLIRP antibodies, custom preparations, or technical support, contact us at email.