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SPP1

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Full Name
Secreted Phosphoprotein 1
Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Biological Process
Biological Process androgen catabolic processManual Assertion Based On ExperimentIDA:CAFA
Biological Process biomineral tissue developmentIEA:UniProtKB-KW
Biological Process cell adhesionManual Assertion Based On ExperimentIDA:ARUK-UCL
Biological Process cellular response to testosterone stimulusManual Assertion Based On ExperimentIDA:CAFA
Biological Process decidualizationManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process embryo implantationManual Assertion Based On ExperimentTAS:BHF-UCL
Biological Process inflammatory responseIEA:Ensembl
Biological Process negative regulation of collateral sprouting of intact axon in response to injuryIEA:Ensembl
Biological Process osteoblast differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of bone resorptionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:CAFA
Biological Process positive regulation of estradiol secretionManual Assertion Based On ExperimentIDA:CAFA
Biological Process response to macrophage colony-stimulating factorIEA:Ensembl
Biological Process response to steroid hormoneIEA:Ensembl
Biological Process response to vitamin DManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular Location
Secreted
PTM
Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties.
Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, PubMed:22582013, PubMed:26091039).
The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity).
O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.

Anti-SPP1 antibodies

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Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXS-6179
Application*: IH
Target: SPP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Mouse, Human
Clone: CBXS-5473
Application*: F, IC, IH, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXS-5163
Application*: P, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBXS-4804
Application*: WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXS-4705
Application*: WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXS-4577
Application*: P, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBXS-4447
Application*: E, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBXS-4101
Application*: E, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBXS-3724
Application*: E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBXS-3663
Application*: F
Target: SPP1
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: CBXS-3601
Application*: IH, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: CBXS-5911
Application*: WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXS-5870
Application*: E, IC, P, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXS-5789
Application*: WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBXS-3866
Application*: IC, P, IP, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2
Specificity: Human
Clone: CBXO-0245
Application*: WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBXO-0236
Application*: E, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBXO-0235
Application*: E, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: CBXO-0221
Application*: E, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 53
Application*: WB, E, IH, P
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBXO-0191
Application*: E, WB, P
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse
Clone: 2A1
Application*: WB, E, IP, IF, IH
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse
Clone: 1H3
Application*: WB, E, IP, B
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse
Clone: 3D9
Application*: WB, E, IP
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse
Clone: 10F6
Application*: WB, E, IP
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 1G4
Application*: WB, E, IP, IH
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBXO-0505
Application*: E, IH, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 2E11
Application*: E, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: X1
Application*: E, IH, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBXO-0222
Application*: F
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: CBXO-0220
Application*: E, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: CBXO-0117
Application*: E, WB, P, IF
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2
Specificity: Human
Clone: 20Z7
Application*: WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human, Mouse
Clone: CBXO-0498
Application*: WB, IF
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 14C08
Application*: SE
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Rat, Mouse
Clone: M574
Application*: WB, E, IC
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBXO-0477
Application*: F
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: CBXO-0116
Application*: E, WB, P
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 5E4
Application*: WB, IH
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBXO-0368
Application*: IH
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 7C5H12
Application*: IC, IF, IH, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse
Clone: 4H7
Application*: E, WB
Target: SPP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat
Clone: CBXO-0503
Application*: F, IC, IF, WB
Target: SPP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: CBXO-0502
Application*: F, IC, IF, WB
Target: SPP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBXO-0189
Application*: WB, E, F, IP
Target: SPP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBXO-0171
Application*: E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 2F10
Application*: IC, IF, P, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse
Clone: 2C5
Application*: WB, E, IP, IH, B
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 2H9
Application*: E, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 1F11
Application*: E, WB
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBXO-0371
Application*: WB
Target: SPP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Mouse, Human
Clone: CBXO-0141
Application*: WB, F, IF
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Rat, Rabbit
Clone: 1B20
Application*: WB, IH, IF, IP, P, C
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CBXO-0213
Application*: F
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: CB180A
Application*: ELISA, WB, IHC
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 4G12
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 4F2
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 4D8
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 4C4
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 4B8
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 4B2
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 3G6
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 3C7
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human, Mouse
Clone: 3B11
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse
Clone: 2D12
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse
Clone: 1F5
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse
Clone: 1D7
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse
Clone: 1D4
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human, Mouse
Clone: 1A7
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 3E11
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 1E10
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 4E6
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 1E6
Application*: WB, E
Target: SPP1
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 1B2C2
Application*: E, WB, IH
Target: SPP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Rat, Human, Rabbit, Mouse
Clone: MPIIIB10(1)
Application*: IF, IF-cells, IH
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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