SPP1 Antibodies

Structure of SPP1

SPP1 (secretory phosphoprotein 1, also known as osteopontin) is a secretory glycoprotein with a molecular weight of approximately 44-75 kDa. Its precise molecular weight varies slightly due to species differences and post-translational modifications such as phosphorylation and glycosylation. This protein is composed of approximately 300 amino acids and has a typical RGD cell adhesion sequence as well as multiple functional domains. Its tertiary structure presents an extended flexible conformation and can mediate cell signal transduction through the binding of integrins and CD44 receptors. SPP1 of different species vary in amino acid sequence and modification degree, thereby affecting their interaction with ligands and biological functions.

Fig. 1 Association between SPP1 and immune cells.¹

Key structural properties of SPP1:

• With stretch type flexible structure domain, including RGD sequence cell adhesion
• Multiple phosphorylation sites and calcium ion binding regions
• Signal transduction is mediated by the binding of integrins (such as αvβ3) to CD44 receptors

Functions of SPP1

The main function of SPP1 (osteopontin) is to mediate the interaction between cells and the matrix and regulate the immune response. In addition, it is widely involved in multiple pathophysiological processes such as tissue repair, tumorigenesis and biomineralization.

SPP1 activates downstream signaling pathways (such as Akt and MAPK pathways) through multiple receptors (such as integrin αvβ3 and CD44), thereby exerting pleiotonic regulatory functions in different microenvironments. Its expression level is closely related to tissue fibrosis, autoimmune diseases and cancer prognosis.

Applications of SPP1 and SPP1 Antibody in Literature

1. De Schepper, Sebastiaan, et al. "Perivascular cells induce microglial phagocytic states and synaptic engulfment via SPP1 in mouse models of Alzheimer's disease." Nature neuroscience 26.3 (2023): 406-415. https://doi.org/10.1038/s41593-023-01257-z

Research has found that SPP1 is mainly secreted by perivascular cells, which can activate the phagocytic function of microglia and mediate their synaptic clearance, leading to synaptic loss in Alzheimer's disease models. Inhibiting SPP1 can effectively prevent synaptic damage.

2. Liu, angxiang, et al. "ANGPTL2+ cancer-associated fibroblasts and SPP1+ macrophages are metastasis accelerators of colorectal cancer." Frontiers in immunology 14 (2023): 1185208. https://doi.org/10.3389/fimmu.2023.1185208

Studies have found that SPP1 is highly expressed in liver metastases of colorectal cancer and is mainly enriched in tumor-associated macrophages. SPP1+ macrophages significantly promote the metastasis of colorectal cancer cells by regulating pathways such as epithelial-mesenchymal transition and are potential therapeutic targets.

3. Zhao, Yanli, et al. "Osteopontin/SPP1: a potential mediator between immune cells and vascular calcification." Frontiers in immunology 15 (2024): 1395596. https://doi.org/10.3389/fimmu.2024.1395596

Research has found that SPP1 is highly expressed in immune cells such as macrophages and is a key factor driving vascular calcification. It promotes the calcification of atherosclerotic plaques by mediating inflammatory responses, leading to heart failure, and can serve as a potential therapeutic target.

4. Hoeft, Konrad, et al. "Platelet-instructed SPP1+ macrophages drive myofibroblast activation in fibrosis in a CXCL4-dependent manner." Cell reports 42.2 (2023). https://doi.org/10.1016/j.celrep.2023.112131

The article indicates that SPP1 is highly expressed in immune cells such as macrophages and is a key factor driving vascular calcification. It promotes the calcification of atherosclerotic plaques by mediating inflammatory responses, leading to heart failure, and can serve as a potential therapeutic target.

5. Matsubara, Eri, et al. "The significance of SPP1 in lung cancers and its impact as a marker for protumor tumor-associated macrophages." Cancers 15.8 (2023): 2250. https://doi.org/10.3390/cancers15082250

Research has found that SPP1 (osteopontin) is a novel marker of tumor-associated macrophages (TAMs), and its expression in lung adenocarcinoma is significantly associated with poor prognosis and chemotherapy resistance. The SPP1/CD44 axis may become a key mechanism for the interaction between cancer cells and Tams.

Creative Biolabs: SPP1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality SPP1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom SPP1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our SPP1 antibodies, custom preparations, or technical support, contact us at email.