ssDNA Antibodies

Structure of ssDNA

ssDNA is a nucleic acid polymer with a relatively small molecular weight, typically ranging from several thousand to several hundred thousand daltons. The specific value varies depending on the chain length and base composition. There are certain differences in the molecular weight of ssDNA from different sources:

ssDNA is composed of a nucleotide chain linked by a phosphodiester bond, and its primary structure is based on the order of base arrangement, which determines the hybridization specificity. Molecules can form secondary structures such as hairpins and stem rings, and these folding patterns are crucial for their recognition and binding to target molecules. ssDNA achieves specific binding to target sequences through base complementary pairing, a property that is widely applied in gene editing, molecular probes, and amplification techniques. Its biological function does not require the participation of cofactors, and its stability mainly depends on sequence length, GC content and environmental conditions.

Fig. 1Schematic representation of the selection process for high‐affinity ssDNA sequences on SWCNT surfaces.¹

Key structural properties of ssDNA:

• Linear nucleotide single-stranded structure
• Stability is maintained through base stacking force and hydrogen bonds
• Targeted binding and recognition are achieved through base complementary pairing

Functions of ssDNA

The core function of ssDNA is to serve as a carrier of genetic information and a medium for gene regulation, and it also plays a key role in various molecular biological processes.

Compared with double-stranded DNA, ssDNA has higher flexibility and can participate in reactions without unwinding, demonstrating faster hybridization kinetics and good programmability, making it an important tool in molecular diagnostics, synthetic biology and therapeutics.

Applications of ssDNA and ssDNA Antibody in Literature

1. Lee, Dakyeon, et al. "Systematic Selection of High‐Affinity ssDNA Sequences to Carbon Nanotubes." Advanced Science 11.32 (2024): 2308915. https://doi.org/10.1002/advs.202308915

In this study, ssDNA sequences with high affinity for single-walled carbon nanotubes were obtained through high-throughput screening, revealing their key base compositions and hydrogen bond interaction mechanisms. A predictive model was constructed by combining molecular simulation and machine learning, providing a new strategy for the design of stable and sensitive biosensors.

2. Liang, Chih-Chao, et al. "Mechanism of single-stranded DNA annealing by RAD52–RPA complex." Nature 629.8012 (2024): 697-703. https://doi.org/10.1038/s41586-024-07347-7

This study reveals the molecular mechanism by which RAD52 binds to RPA by forming an open-loop structure, driving the annealing of complementary ssDNA. Structural analysis indicates that ssDNA is located in the positively charged channel of the RAD52 ring, with its N-terminal domain promoting annealing and the C-terminal regulating the open-ring conformation and RPA interaction. This discovery provides new insights into targeting RAD52 for the treatment of homologous recombination deficiency cancers.

3. Park, Jee-Woong, et al. "Acousto-microfluidics for screening of ssDNA aptamer." Scientific reports 6.1 (2016): 27121. https://doi.org/10.1038/srep27121

In this study, acoustic fluid screening technology was employed in combination with high-throughput sequencing to successfully obtain ssDNA aptamers that bind to prostate-specific antigen (PSA) with high affinity. The dissociation constant of the best candidate molecule reaches 0.7 nM. This method does not require the traditional washing step, significantly improving the screening efficiency and specificity.

4. Okorokov, Andrei L., et al. "Structure of the hDmc1-ssDNA filament reveals the principles of its architecture." PloS one 5.1 (2010): e8586. https://doi.org/10.1371/journal.pone.0008586

In this study, the structure of the pre-synaptic complex formed by human Dmc1 protein and ssDNA was resolved by single-particle electron microscopy. Unlike the RecA/Rad51 family, hDmc1 forms an extended active filament with 9 subunits per circle on ssDNA, each subunit covering two nucleotides, and its N-terminal domain coordinates the role of adjacent organisms through domain exchange mechanisms.

5. Ribeiro, Jonathan, et al. "RPA homologs and ssDNA processing during meiotic recombination." Chromosoma 125.2 (2016): 265-276. https://doi.org/10.1007/s00412-015-0552-7

The article indicates that in meiosis homologous recombination, the formation and maturation of ssDNA are key links. Unlike mitosis, MEIOB and multiple RPA subunits work in synergy to specifically regulate ssDNA processing, ensuring that homologous chromosomes preferentially serve as repair templates, thereby maintaining genomic stability and genetic diversity.

Creative Biolabs: ssDNA Antibodies for Research

Creative Biolabs specializes in the production of high-quality ssDNA antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for PCR, gene editing, sequencing, and nucleic acid hybridization, and other diagnostic methodologies.

• Custom ssDNA Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our ssDNA antibodies, custom preparations, or technical support, contact us at email.