Why do I see multiple bands in my Western Blot when using this antibody?

Observing multiple bands in a Western Blot can be due to several factors. These may include post-translational modifications (e.g., phosphorylation, glycosylation), alternative splicing that produces different-sized protein isoforms, or the formation of protein multimers. Ensure you are using the correct dilution and follow the recommended protocol to minimize non-specific binding.

Is this antibody suitable for use in human samples?

The Mouse Anti-ACSL5 Recombinant Antibody (V2-179445) is specifically designed and validated for mouse applications. While ACSL5 sequences can be highly conserved across species, we cannot guarantee cross-reactivity with human samples. For human-specific applications, we recommend checking the datasheet for species reactivity or contacting our technical support team for a suitable alternative.

Mouse Anti-ACSL5 Recombinant Antibody (V2-179445) (CBMAB-A0752-YC)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

V2-179445

Application

ELISA, IHC, IP, WB

Immunogen

Partial recombinant corresponding to aa91-187, from human ACSL5 (NP_057318, 51703) with GST tag.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.2

Buffer

PBS, pH7.2

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acyl-CoA Synthetase Long Chain Family Member 5

Introduction

ACSL5 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA

Entrez Gene ID

51703

UniProt ID

Q9ULC5

Alternative Names

Acyl-CoA Synthetase Long Chain Family Member 5; Fatty-Acid-Coenzyme A Ligase, Long-Chain 5; Long-Chain Acyl-CoA Synthetase 5; Long-Chain Fatty Acid Coenzyme A Ligase 5; FACL5 For Fatty Acid Coenzyme A Ligase 5; EC 6.2.1.3; LACS 5;

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells.

Biological Process

Lipid biosynthetic process
Long-chain fatty acid metabolic process
Long-chain fatty-acyl-CoA biosynthetic process
Positive regulation of long-chain fatty acid import across plasma membrane
Regulation of extrinsic apoptotic signaling pathway

Cellular Location

Cell membrane; Mitochondrion; Mitochondrion outer membrane; Endoplasmic reticulum; Endoplasmic reticulum membrane

Topology

Helical: 12-32 aa
Cytoplasmic: 33-683 aa

Zhang, L., Lv, J., Chen, C., & Wang, X. (2021). Roles of acyl-CoA synthetase long-chain family member 5 and colony stimulating factor 2 in inhibition of palmitic or stearic acids in lung cancer cell proliferation and metabolism. Cell biology and toxicology, 37(1), 15-34.

Al‐Thihli, K., Afting, C., Al‐Hashmi, N., Mohammed, M., Sliwinski, S., Al Shibli, N., ... & Al‐Maawali, A. (2021). Deficiency of acyl‐CoA synthetase 5 is associated with a severe and treatable failure to thrive of neonatal onset. Clinical Genetics, 99(3), 376-383.

Gharib, E., Nasri Nasrabadi, P., & Reza Zali, M. (2020). miR‐497‐5p mediates starvation‐induced death in colon cancer cells by targeting acyl‐CoA synthetase‐5 and modulation of lipid metabolism. Journal of cellular physiology, 235(7-8), 5570-5589.

O’Brien, M. J., Beijerink, N. J., Sansom, M., Thornton, S. W., Chew, T., & Wade, C. M. (2020). A large deletion on CFA28 omitting ACSL5 gene is associated with intestinal lipid malabsorption in the Australian Kelpie dog breed. Scientific reports, 10(1), 1-10.

Yu, X., Fang, X., Xiao, H., Zhao, Z., Maak, S., Wang, M., & Yang, R. (2019). The effect of acyl-CoA synthetase long-chain family member 5 on triglyceride synthesis in bovine preadipocytes. Archives animal breeding, 62(1), 257-264.

Kwak, H. B., Woodlief, T. L., Green, T. D., Cox, J. H., Hickner, R. C., Neufer, P. D., & Cortright, R. N. (2019). Overexpression of long-chain acyl-CoA synthetase 5 increases fatty acid oxidation and free radical formation while attenuating insulin signaling in primary human skeletal myotubes. International journal of environmental research and public health, 16(7), 1157.

Rossi Sebastiano, M., & Konstantinidou, G. (2019). Targeting long chain acyl-coa synthetases for cancer therapy. International journal of molecular sciences, 20(15), 3624.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ACSL5 Matched Antibody Pair (007) (CAT#: APMAB-007LY)

Related Products

ACSL5 Matched Antibody Pair (007) (CAT#: APMAB-007LY)

Mouse Anti-ACSL5 Recombinant Antibody (V2-179446) (CAT#: CBMAB-A0753-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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