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Mouse Anti-ACVRL1 Recombinant Antibody (V2-179704) (CBMAB-A1064-YC)

Provided herein is a Mouse monoclonal antibody against Human Activin A Receptor Like Type 1. The antibody can be used for immunoassay techniques, such as WB.
See all ACVRL1 antibodies
Published Data
Mouse Anti-ACVRL1 Recombinant Antibody (CBYC-A289) (CBMAB-A1064-YC) in WB
ALK1 and ALK5 protein expression. C28/I2 cells were transfected with ALK1, ALK5, or empty vector, and the cell lysates were analyzed by Western blotting using anti-ALK1 (Abgent; top panel) or anti-ALK5 (Cell Signaling Technologies; middle panel) antibodies. The membrane was reprobed with an anti-actin antibody to confirm equal protein loading (bottom panel). ...View More
Finnson, K. W., Parker, W. L., ten Dijke, P., Thorikay, M., & Philip, A. (2008). ALK1 opposes ALK5/Smad3 signaling and expression of extracellular matrix components in human chondrocytes. Journal of bone and mineral research, 23(6), 896-906.
Fig.3 Staining for ALK1 (mab) in pancreatic carcinoma tissues.
...View More
Fig.4 ALK1 protein is expressed in PANC-1 cells.
...View More
Mouse Anti-ACVRL1 Recombinant Antibody (CBYC-A289) (CBMAB-A1064-YC) in Immunoblot
ALK1 expression in COLO-357 clones from detected by combining IP and IB as described for PANC-1. ...View More
Ungefroren, H., Schniewind, B., Groth, S., Chen, W. B., Müerköster, S. S., Kalthoff, H., & Fändrich, F. (2007). Antitumor activity of ALK1 in pancreatic carcinoma cells. International journal of cancer, 120(8), 1641-1651.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179704
Antibody Isotype
IgG2a
Application
WB, FC

Basic Information

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALK-1, Asp22-Gln118.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB2 μg/ml
Simple Western40 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Activin A Receptor Like Type 1
Introduction
ACVRL1 is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding t
Entrez Gene ID
94
UniProt ID
P37023
Alternative Names
ALK1; HHT; ORW2; ALK-1; ACVRLK1; HHT2; SKR3; TSR-I
Function
Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
Biological Process
Angiogenesis
Artery development
Blood circulation
Blood vessel endothelial cell proliferation involved in sprouting angiogenesis
Blood vessel maturation
Blood vessel remodeling
BMP signaling pathway
Cellular response to BMP stimulus
Cellular response to growth factor stimulus
Cellular response to transforming growth factor beta stimulus
Dorsal/ventral pattern formation
Dorsal aorta morphogenesis
Endocardial cushion morphogenesis
Endothelial tube morphogenesis
Heart development
Lymphangiogenesis
Lymphatic endothelial cell differentiation
Negative regulation of blood vessel endothelial cell migration
Negative regulation of cell adhesion
Negative regulation of cell growth
Negative regulation of cell migration
Negative regulation of cell population proliferation
Negative regulation of DNA biosynthetic process
Negative regulation of endothelial cell migration
Negative regulation of focal adhesion assembly
Negative regulation of gene expression
Positive regulation of BMP signaling pathway
Positive regulation of chondrocyte differentiation
Positive regulation of pathway-restricted SMAD protein phosphorylation
Positive regulation of transcription, DNA-templated
Positive regulation of transcription by RNA polymerase II
Protein phosphorylation
Regulation of blood pressure
Regulation of blood vessel endothelial cell migration
Regulation of DNA replication
Regulation of endothelial cell proliferation
Regulation of transcription, DNA-templated
Retina vasculature development in camera-type eye
Signal transduction
Transforming growth factor beta receptor signaling pathway
Venous blood vessel development
Wound healing, spreading of epidermal cells
Cellular Location
Cell membrane
Involvement in disease
Telangiectasia, hereditary hemorrhagic, 2 (HHT2): A multisystemic vascular dysplasia leading to dilation of permanent blood vessels and arteriovenous malformations of skin, mucosa, and viscera. The disease is characterized by recurrent epistaxis and gastro-intestinal hemorrhage. Visceral involvement includes arteriovenous malformations of the lung, liver, and brain.
Topology
Extracellular: 22-118 aa
Helical: 119-141 aa
Cytoplasmic: 142-503 aa

Toyonaga, T., Steinbach, E. C., Keith, B. P., Barrow, J. B., Schaner, M. R., Wolber, E. A., ... & Sheikh, S. Z. (2020). Decreased colonic Activin receptor-like kinase 1 disrupts epithelial barrier integrity in patients with Crohn’s disease. Cellular and molecular gastroenterology and hepatology, 10(4), 779-796.

Anderson, K. E., Bellio, T. A., Aniskovich, E., Adams, S. L., Blusztajn, J. K., & Delalle, I. (2020). The Expression of Activin Receptor-Like Kinase 1 (ACVRL1/ALK1) in Hippocampal Arterioles Declines During Progression of Alzheimer’s Disease. Cerebral cortex communications, 1(1), tgaa031.

Mamoor, S. (2020). The Activin-A receptor-like protein 1, ACVRL1 is differentially expressed in non-small cell lung cancer.

Hwan Kim, Y., Vu, P. N., Choe, S. W., Jeon, C. J., Arthur, H. M., Vary, C. P., ... & Oh, S. P. (2020). Overexpression of activin receptor-like kinase 1 in endothelial cells suppresses development of arteriovenous malformations in mouse models of hereditary hemorrhagic telangiectasia. Circulation research, 127(9), 1122-1137.

Bocci, M., Sjölund, J., Kurzejamska, E., Lindgren, D., Bartoschek, M., Höglund, M., & Pietras, K. (2019). Activin receptor-like kinase 1 is associated with immune cell infiltration and regulates CLEC14A transcription in cancer. Angiogenesis, 22(1), 117-131.

Adams, S. L., Benayoun, L., Tilton, K., Mellott, T. J., Seshadri, S., Blusztajn, J. K., & Delalle, I. (2018). Immunohistochemical analysis of activin receptor-like kinase 1 (ACVRL1/ALK1) expression in the rat and human hippocampus: Decline in CA3 during progression of Alzheimer’s disease. Journal of Alzheimer's Disease, 63(4), 1433-1443.

Claesson-Welsh, L. (2018). Alk1 (Activin Receptor-Like Kinase 1) and Vascular Hyperpermeability in Diabetic Retinopathy: More Is Less.

Busceti, C. L., Marchitti, S., Bianchi, F., Di Pietro, P., Riozzi, B., Stanzione, R., ... & Rubattu, S. (2017). Dickkopf-3 upregulates VEGF in cultured human endothelial cells by activating activin receptor-like kinase 1 (ALK1) pathway. Frontiers in pharmacology, 8, 111.

Roman, B. L., & Hinck, A. P. (2017). ALK1 signaling in development and disease: new paradigms. Cellular and Molecular Life Sciences, 74(24), 4539-4560.

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For research use only. Not intended for any clinical use.

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