Mouse Anti-ADRA2B Recombinant Antibody (V2-180022) (CBMAB-A1415-YC)

Provided herein is a Mouse monoclonal antibody against Mouse Adrenoceptor Alpha 2B. The antibody can be used for immunoassay techniques, such as IHC-Fr, WB.
See all ADRA2B antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse, Rat

Clone

V2-180022

Antibody Isotype

IgG1

Application

Basic Information

Immunogen

Synthetic peptide internal sequence corresponding to the thirdintracellular loop of rat alpha 2b adrenergic receptor

Host Species

Mouse

Specificity

Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	1-5 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS

Preservative

0.08% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Adrenoceptor Alpha 2B

Introduction

This intronless gene encodes a seven-pass transmembrane protein. This protein is a member of a subfamily of G protein-coupled receptors that regulate neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous syste

Entrez Gene ID

Mouse	11552
Rat	24174

UniProt ID

Mouse	P30545
Rat	P19328

Alternative Names

Adrenoceptor Alpha 2B; Alpha-2 Adrenergic Receptor Subtype C2; Alpha-2B Adrenoreceptor; Alpha-2BAR; ADRA2RL1; ADRA2L1; ADRA2B Adrenergic, Alpha-2B-, Receptor; Alpha-2-Adrenergic Receptor-Like 1;

Function

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is clonidine> norepinephrine> epinephrine = oxymetazoline> dopamine> p-tyramine = phenylephrine> serotonin> p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine> chlorpromazine> phentolamine> mianserine> spiperone> prazosin> alprenolol> propanolol> pindolol.

Biological Process

Activation of MAPK activity
Activation of protein kinase B activity
Adenylate cyclase-activating adrenergic receptor signaling pathway
Adrenergic receptor signaling pathway
Cell-cell signaling
Female pregnancy
G protein-coupled receptor signaling pathway
Negative regulation of epinephrine secretion
Negative regulation of norepinephrine secretion
Platelet activation
Positive regulation of blood pressure
Positive regulation of MAPK cascade
Positive regulation of neuron differentiation
Positive regulation of uterine smooth muscle contraction
Receptor transactivation
Regulation of vascular associated smooth muscle contraction

Cellular Location

Cell membrane. Interaction with RAB26, GGA1, GGA2 and GGA3 mediates transport from the Golgi to the cell membrane.

Involvement in disease

Epilepsy, familial adult myoclonic, 2 (FAME2): A form of familial myoclonic epilepsy, a neurologic disorder characterized by cortical hand tremors, myoclonic jerks and occasional generalized or focal seizures with a non-progressive or very slowly progressive disease course. Usually, myoclonic tremor is the presenting symptom, characterized by tremulous finger movements and myoclonic jerks of the limbs increased by action and posture. In a minority of patients, seizures are the presenting symptom. Some patients exhibit mild cognitive impairment. FAME2 inheritance is autosomal dominant.

Topology

Extracellular: 1-12 aa
Helical: 13-38 aa
Cytoplasmic: 39-48 aa
Helical: 49-69 aa
Extracellular: 70-86 aa
Helical: 87-107 aa
Cytoplasmic: 108-128 aa
Helical: 129-149 aa
Extracellular: 150-172 aa
Helical: 173-193 aa
Cytoplasmic: 194-368 aa
Helical: 369-389 aa
Extracellular: 390-405 aa
Helical: 406-426 aa
Cytoplasmic: 427-450 aa

References

Rao, X., Asico, L. D., Zanos, P., Mahabeleshwar, G. H., Singh Gangwar, R., Xia, C., ... & Rajagopalan, S. (2019). Alpha2B-adrenergic receptor overexpression in the brain potentiate air pollution-induced behavior and blood pressure changes. Toxicological Sciences, 169(1), 95-107.

Melnikov, V. N., Baranov, V. I., Suvorova, I. Y., & Krivoschekov, S. G. (2018). Associations of Del 301-303 alpha2B-adrenoceptor gene polymorphism with central hemodynamic parameters in the northern Russian population. Physiological genomics, 50(2), 100-101.

Xie, W., Cappiello, M., Meng, M., Rosenthal, R., & Zhang, W. (2018). ADRA2B deletion variant and enhanced cognitive processing of emotional information: A meta-analytical review. Neuroscience & Biobehavioral Reviews, 92, 402-416.

Wirz, L., Wacker, J., Felten, A., Reuter, M., & Schwabe, L. (2017). A deletion variant of the α2b-adrenoceptor modulates the stress-induced shift from “cognitive” to “habit” memory. Journal of Neuroscience, 37(8), 2149-2160.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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