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Rat Anti-ANGPTL3 Recombinant Antibody (CBYC-A597) (CBMAB-A2719-YC)

Provided herein is a Mouse monoclonal antibody against Human Angiopoietin Like 3. The antibody can be used for immunoassay techniques, such as WB.
See all ANGPTL3 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYC-A597
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Angiopoietin-like 3 Ser17-Thr455.
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Angiopoietin Like 3
Introduction
ANGPTL3 is a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen
Entrez Gene ID
27329
UniProt ID
Q9Y5C1
Alternative Names
Angiopoietin Like 3; Angiopoietin 5; ANGPT5; ANG-5; Angiopoietin-Related Protein 3; Angiopoietin-Like Protein 3;
Function
Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism (PubMed:11788823, PubMed:12909640, PubMed:23661675, PubMed:25495645). Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake (By similarity). Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1 (PubMed:12097324, PubMed:19318355, PubMed:20581395). Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids (PubMed:17110602, PubMed:19028676). Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol (PubMed:12565906). Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food; the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT (By similarity). Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR. May stimulate hypothalamic LPL activity (By similarity).
ANGPTL3(17-221):In vitro inhibits LPL activity; not effective on GPIHBP1-stabilized LPL.
Involved in angiogenesis. Binds to endothelial cells via integrin alpha-V/beta-3 (ITGAV:ITGB3), activates FAK, MAPK and Akt signaling pathways and induces cell adhesion and cell migration (PubMed:11877390). Secreted from podocytes, may modulate properties of glomerular endothelial cells involving integrin alpha-V/beta-3 and Akt signaling (PubMed:18535744). May increase the motility of podocytes. May induce actin filament rearrangements in podocytes implicating integrin alpha-V/beta-3 and Rac1 activation. Binds to hematopoietic stem cells (HSC) and is involved in the regulation of HSC activity probably implicating down-regulation of IKZF1/IKAROS (By similarity).
Biological Process
Acylglycerol homeostasis Source: BHF-UCL
Angiogenesis Source: UniProtKB-KW
Artery morphogenesis Source: BHF-UCL
Cell-matrix adhesion Source: UniProtKB
Cholesterol homeostasis Source: BHF-UCL
Cholesterol metabolic process Source: BHF-UCL
Fatty acid metabolic process Source: BHF-UCL
Glycerol metabolic process Source: BHF-UCL
Integrin-mediated signaling pathway Source: UniProtKB
Intracellular receptor signaling pathway Source: Reactome
Lipid homeostasis Source: BHF-UCL
Lipid storage Source: BHF-UCL
Negative regulation of lipoprotein lipase activity Source: BHF-UCL
Negative regulation of phospholipase activity Source: BHF-UCL
Phospholipid catabolic process Source: BHF-UCL
Phospholipid homeostasis Source: BHF-UCL
Phospholipid metabolic process Source: BHF-UCL
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of lipid catabolic process Source: BHF-UCL
Regulation of lipoprotein lipase activity Source: Reactome
Response to hormone Source: Ensembl
Signal transduction Source: BHF-UCL
Triglyceride homeostasis Source: MGI
Cellular Location
Secreted; Lamellipodium. Colocalized with HSPG2 and activated ITGB3 on podocytes.
Involvement in disease
Hypobetalipoproteinemia, familial, 2 (FHBL2): A disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.
PTM
O-glycosylated at Thr-226 by GALNT2; blocks processing and activation by proprotein convertases.
In part proteolytically cleaved by proprotein convertases; proposed to be involved in activation.

Ling, P., Zheng, X., Luo, S., Ge, J., Xu, S., & Weng, J. (2021). Targeting angiopoietin‐like 3 (ANGPTL3) in atherosclerosis: from bench to bedside. Diabetes, Obesity and Metabolism.

Lu, X. (2020). Structure and function of angiopoietin-like protein 3 (ANGPTL3) in atherosclerosis. Current medicinal chemistry, 27(31), 5159-5174.

Vatannejad, A., Salimi, F., Moradi, N., Fouani, F. Z., Zandieh, Z., Ansaripour, S., ... & Fadaei, R. (2020). Evaluation of angiopoietin-like protein 3 (ANGPTL3) levels in polycystic ovary syndrome. Life Sciences, 263, 118595.

Watts, G. F., Schwabe, C., Scott, R., Gladding, P., Sullivan, D., Baker, J., ... & Ballantyne, C. (2020). RNAi inhibition of angiopoietin-like protein 3 (ANGPTL3) with ARO-ANG3 mimics the lipid and lipoprotein profile of familial combined hypolipidemia. European Heart Journal, 41(Supplement_2), ehaa946-3331.

Adam, R. C., Mintah, I. J., Alexa-Braun, C. A., Shihanian, L. M., Lee, J. S., Banerjee, P., ... & Gusarova, V. (2020). Angiopoietin-like protein 3 governs LDL-cholesterol levels through endothelial lipase-dependent VLDL clearance. Journal of lipid research, 61(9), 1271-1286.

Wang, X., & Musunuru, K. (2019). Angiopoietin-like 3: from discovery to therapeutic gene editing. JACC: Basic to Translational Science, 4(6), 755-762.

Tarugi, P., Bertolini, S., & Calandra, S. (2019). Angiopoietin-like protein 3 (ANGPTL3) deficiency and familial combined hypolipidemia. Journal of biomedical research, 33(2), 73.

Lupo, M. G., & Ferri, N. (2018). Angiopoietin-like 3 (ANGPTL3) and atherosclerosis: lipid and non-lipid related effects. Journal of cardiovascular development and disease, 5(3), 39.

Xu, Y. X., Redon, V., Yu, H., Querbes, W., Pirruccello, J., Liebow, A., ... & Kathiresan, S. (2018). Role of angiopoietin-like 3 (ANGPTL3) in regulating plasma level of low-density lipoprotein cholesterol. Atherosclerosis, 268, 196-206.

Kersten, S. (2017). Angiopoietin-like 3 in lipoprotein metabolism. Nature Reviews Endocrinology, 13(12), 731-739.

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For research use only. Not intended for any clinical use.

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