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Rat Anti-APC Recombinant Antibody (KT44) (CBMAB-1095-CN)

This product is a rat antibody that recognizes APC of mouse. The antibody KT44 can be used for immunoassay techniques such as: WB, IF.
See all APC antibodies

Summary

Host Animal
Rat
Specificity
Human, Mouse, Rat
Clone
KT44
Antibody Isotype
IgG2a
Application
WB, IF

Basic Information

Immunogen
Adenomatous polyposis coli protein fused to GST (aa. 788-1038).
Host Species
Rat
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:250
IF(ICC)1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
APC, WNT Signaling Pathway Regulator
Introduction
This protein promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. It plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
Entrez Gene ID
11789
UniProt ID
Q61315
Alternative Names
CC1; Min; mAPC; AI047805; AU020952; AW124434
Function
Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
Biological Process
Beta-catenin destruction complex assembly Source: Reactome
Beta-catenin destruction complex disassembly Source: Reactome
Bicellular tight junction assembly Source: UniProtKB
Cell adhesion Source: UniProtKB
Cell cycle arrest Source: UniProtKB
Cell fate specification Source: GO_Central
Cell migration Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Insulin receptor signaling pathway Source: CAFA
Mitotic cytokinesis Source: MGI
Mitotic spindle assembly checkpoint Source: MGI
Negative regulation of canonical Wnt signaling pathway Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
Negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
Negative regulation of microtubule depolymerization Source: UniProtKB
Nervous system development Source: GO_Central
Pattern specification process Source: GO_Central
Positive regulation of apoptotic process Source: MGI
Positive regulation of cell death Source: CAFA
Positive regulation of cell migration Source: MGI
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Positive regulation of protein catabolic process Source: MGI
Positive regulation of protein localization to centrosome Source: CAFA
Positive regulation of pseudopodium assembly Source: MGI
Protein-containing complex assembly Source: UniProtKB
Protein deubiquitination Source: Reactome
Regulation of attachment of spindle microtubules to kinetochore Source: MGI
Regulation of cell differentiation Source: GO_Central
Regulation of microtubule-based process Source: UniProtKB
Wnt signaling pathway Source: Reactome
Cellular Location
Cytoplasm; Ruffle membrane; Cell membrane; Cytoskeleton; Adherens junction; Lamellipodium. Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184). Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment (PubMed:19151759). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854).
Involvement in disease
Familial adenomatous polyposis 1 (FAP1): A cancer predisposition syndrome characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years.
Desmoid disease, hereditary (DESMD): An autosomal dominant disease characterized by multifocal fibromatosis of the abdominal wall and mesentery. Desmoid tumors can also affect paraspinal muscles, breast, occiput, arms, and lower ribs.
Medulloblastoma (MDB): Malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Gastric cancer (GASC): A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
Hepatocellular carcinoma (HCC): A primary malignant neoplasm of epithelial liver cells. The major risk factors for HCC are chronic hepatitis B virus (HBV) infection, chronic hepatitis C virus (HCV) infection, prolonged dietary aflatoxin exposure, alcoholic cirrhosis, and cirrhosis due to other causes.
PTM
Phosphorylated by GSK3B.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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