Mouse Anti-ARL13B Recombinant Antibody (6F11) (CBMAB-A0478-LY)

Name: Mouse Anti-ARL13B Recombinant Antibody (6F11)
SKU: CBMAB-A0478-LY
Rating: 5 (1 reviews)

See all ARL13B antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6F11

Application

WB, ELISA

Immunogen

ARL13B (NP_878899.1, 329 a.a. ~ 428 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ADP-ribosylation factor-like 13B

Entrez Gene ID

200894

UniProt ID

Q3SXY8

Alternative Names

ARL2L1; DKFZp686E2075; DKFZp686L2472; DKFZp686M2074; DKFZp761H079; JBTS8; MGC120611; MGC120612

Function

Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. May regulate endocytic recycling traffic; however, additional evidence is required to confirm these data.

Biological Process

Aggrephagy Source: Reactome
Cilium assembly Source: UniProtKB
Formation of radial glial scaffolds Source: UniProtKB
Interneuron migration from the subpallium to the cortex Source: UniProtKB
Neural tube patterning Source: UniProtKB
Non-motile cilium assembly Source: UniProtKB
Receptor localization to non-motile cilium Source: GO_Central
Smoothened signaling pathway Source: UniProtKB

Cellular Location

Cilium membrane; Cilium. Associates to the cilium membrane via palmitoylation. Localizes to proximal ciliary membranes, to an inversin-like subciliary membrane compartment, excluding the transition zone.

Involvement in disease

Joubert syndrome 8 (JBTS8): A disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease.

PTM

Sumoylation is required for PKD2 entry into cilium.

Han, S., Miyoshi, K., Shikada, S., Amano, G., Wang, Y., Yoshimura, T., & Katayama, T. (2019). TULP3 is required for localization of membrane-associated proteins ARL13B and INPP5E to primary cilia. Biochemical and biophysical research communications, 509(1), 227-234.

Dilan, T., & Ramamurthy, V. (2019). The dynamic and complex role of the Joubert Syndrome-associated ciliary protein, ADP-ribosylation factor-like GTPase 13B (ARL13B) in photoreceptor development and maintenance. Retinal Degenerative Diseases, 501-505.

Bay, S. N., Long, A. B., & Caspary, T. (2018). Disruption of the ciliary GTPase Arl13b suppresses Sonic hedgehog overactivation and inhibits medulloblastoma formation. Proceedings of the National Academy of Sciences, 115(7), 1570-1575.

Rafiullah, R., Long, A. B., Ivanova, A. A., Ali, H., Berkel, S., Mustafa, G., ... & Rappold, G. A. (2017). A novel homozygous ARL13B variant in patients with Joubert syndrome impairs its guanine nucleotide-exchange factor activity. European Journal of Human Genetics, 25(12), 1324-1334.

Roy, K., Jerman, S., Jozsef, L., McNamara, T., Onyekaba, G., Sun, Z., & Marin, E. P. (2017). Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation. Journal of Biological Chemistry, 292(43), 17703-17717.

Hanke-Gogokhia, C., Wu, Z., Sharif, A., Yazigi, H., Frederick, J. M., & Baehr, W. (2017). The guanine nucleotide exchange factor Arf-like protein 13b is essential for assembly of the mouse photoreceptor transition zone and outer segment. Journal of Biological Chemistry, 292(52), 21442-21456.

Ivanova, A. A., Caspary, T., Seyfried, N. T., Duong, D. M., West, A. B., Liu, Z., & Kahn, R. A. (2017). Biochemical characterization of purified mammalian ARL13B protein indicates that it is an atypical GTPase and ARL3 guanine nucleotide exchange factor (GEF). Journal of Biological Chemistry, 292(26), 11091-11108.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ARL13B Recombinant Antibody (6F11)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry