Rat Anti-Art2b Recombinant Antibody (3H450) (CBMAB-R0424-CN)

This product is a Rat antibody that recognizes Art2b. The antibody 3H450 can be used for immunoassay techniques such as: ELISA, FC, RIA.
See all ART2B antibodies

Summary

Host Animal

Rat

Specificity

Rat

Clone

3H450

Antibody Isotype

IgG2a

Application

FC, IP

Basic Information

Immunogen

pME.CD8LF-ART2.2.

Specificity

Rat

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
FC	1:10-1:1,000
IP	1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

0.09% sodium azide

Concentration

1 mg/ml

Target

Full Name

ADP-ribosyltransferase 2b

Introduction

Has NAD+ glycohydrolase activity and extremely low ADP-ribosyltransferase activity.

Entrez Gene ID

293152

UniProt ID

P17982

Alternative Names

LY2; Rt6; Ag-F; Art2; Ly-2; PtaA; ARTC2; Art2a; RT6.1; RT6.2

Function

Has both NAD+ glycohydrolase and ADP-ribosyltransferase activity (to a lesser extent).

Biological Process

NAD catabolic process Source: UniProtKB
Peptidyl-arginine ADP-ribosylation Source: GO_Central

Cellular Location

Cell membrane

References

Leutert, M., Duan, Y., Winzer, R., Menzel, S., Tolosa, E., Magnus, T., ... & Rissiek, B. (2021). Identification of the mouse T cell ADP-ribosylome uncovers ARTC2. 2 mediated regulation of CD73 by ADP-ribosylation. Frontiers in Immunology, 12.

Rissiek, B., Lukowiak, M., Raczkowski, F., Magnus, T., Mittrücker, H. W., & Koch-Nolte, F. (2018). In vivo blockade of murine ARTC2. 2 during cell preparation preserves the vitality and function of liver tissue-resident memory T cells. Frontiers in immunology, 9, 1580.

Rissiek, B., Menzel, S., Leutert, M., Cordes, M., Behr, S., Jank, L., ... & Magnus, T. (2017). Ecto-ADP-ribosyltransferase ARTC2. 1 functionally modulates FcγR1 and FcγR2B on murine microglia. Scientific reports, 7(1), 1-13.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
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Second antibody and isotype control

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