Sign in or Register   Sign in or Register
  |  

Rabbit Anti-ATG7 Recombinant Antibody (9H196) (CBMAB-A3951-YC)

Provided herein is a Rabbit monoclonal antibody against Human Autophagy Related 7. The antibody can be used for immunoassay techniques, such as WB, IP, IHC, ICC, FC.
See all ATG7 antibodies
Published Data
Rabbit Anti-ATG7 Recombinant Antibody (9H196) (CBMAB-A3951-YC) in ICC
Autophagy pathway proteins, ATG7 (red) was downregulated in CC-HS-treated CFH(Y/Y)-iRPE2, compared to CI-HS treatment; as confirmed by immunostaining. ...View More
Sharma, R., George, A., Nimmagadda, M., Ortolan, D., Karla, B. S., Qureshy, Z., ... & Bharti, K. (2021). Epithelial phenotype restoring drugs suppress macular degeneration phenotypes in an iPSC model. Nature Communications, 12(1), 7293.
Rabbit Anti-ATG7 Recombinant Antibody (9H196) (CBMAB-A3951-YC) in IHC
Representative images of pancreatic tissue from all cohorts stained as indicated. Scale bars: 20 µm. Inserts are higher magnifications of the boxed area. For easier recognition the dashed areas highlight islets in Atg7ind mice. ...View More
Mainz, L., Sarhan, M. A., Roth, S., Sauer, U., Kalogirou, C., Eckstein, M., ... & Rosenfeldt, M. T. (2022). Acute systemic knockdown of Atg7 is lethal and causes pancreatic destruction in shRNA transgenic mice. Autophagy, 18(12), 2880-2893.
Rabbit Anti-ATG7 Recombinant Antibody (9H196) (CBMAB-A3951-YC) in Immunoblot
Immunoblot analysis of SEL1L-HRD1 ERAD and autophagy in gonadal WAT (n = 4 mice per genotype). ...View More
Wu, S. A., Shen, C., Wei, X., Zhang, X., Wang, S., Chen, X., ... & Qi, L. (2023). The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes. Nature Communications, 14(1), 3132.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
9H196
Antibody Isotype
IgG
Application
IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues near the amino terminus of human Atg7 protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10mM HEPES, pH 7.5, 0.15M NaCl, 0.1mg/mL BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 7
Introduction
ATG7 is an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with mul
Entrez Gene ID
10533
UniProt ID
O95352
Alternative Names
Autophagy Related 7; Ubiquitin-Activating Enzyme E1-Like Protein; ATG12-Activating Enzyme E1 ATG7; APG7-LIKE; APG7L; HAGP7; ATG7 Autophagy Related 7 Homolog (S. Cerevisiae);
Function
E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).
Biological Process
Aging Source: Ensembl
Autophagosome assembly Source: GO_Central
Autophagy Source: GO_Central
Autophagy of mitochondrion Source: ParkinsonsUK-UCL
Cellular response to hyperoxia Source: UniProtKB
Cellular response to morphine Source: Ensembl
Cellular response to nitrogen starvation Source: GO_Central
Cellular response to starvation Source: UniProtKB
Chaperone-mediated autophagy Source: Ensembl
C-terminal protein lipidation Source: GO_Central
Defense response to virus Source: CACAO
Late nucleophagy Source: GO_Central
Macroautophagy Source: BHF-UCL
Negative regulation of mitochondrial DNA replication Source: Ensembl
Negative regulation of oxidative stress-induced neuron death Source: Ensembl
Neutrophil degranulation Source: Reactome
Piecemeal microautophagy of the nucleus Source: GO_Central
Positive regulation by symbiont of host autophagy Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of autophagy Source: Ensembl
Positive regulation of protein catabolic process Source: UniProtKB
Positive regulation of protein modification process Source: MGI
Protein lipidation Source: MGI
Protein modification by small protein conjugation Source: GO_Central
Protein transport Source: UniProtKB-KW
Regulation of circadian rhythm Source: UniProtKB
Response to fluoride Source: Ensembl
Response to glucose Source: Ensembl
Rhythmic process Source: UniProtKB-KW
Suppression by virus of host autophagy Source: CACAO
Cellular Location
Cytoplasm; Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
PTM
Acetylated by EP300.

Yang, X., Zhang, L., Ye, J. Q., Wu, X. H., Zeng, X. X., Chen, L. W., & Li, Y. M. (2021). The role of ATG-7 contributes to pulmonary hypertension by impacting vascular remodeling. Journal of Molecular and Cellular Cardiology, 157, 1-13.

Samaka, R. M., Basha, M. A., & Mansour, E. (2020). Does the Autophagy Related Gene 7 (ATG7) Have a Role in Non-Melanoma Skin Cancer?. Clinical, cosmetic and investigational dermatology, 13, 49.

Li, J., Lin, W. E. I., & Zhuang, L. E. I. (2020). CD5L‑induced activation of autophagy is associated with hepatoprotection in ischemic reperfusion injury via the CD36/ATG7 axis. Experimental and therapeutic medicine, 19(4), 2588-2596.

Li, M., Liu, J., Li, S., Feng, Y., Yi, F., Wang, L., ... & Cao, L. (2019). Autophagy-related 7 modulates tumor progression in triple-negative breast cancer. Laboratory Investigation, 99(9), 1266-1274.

Palanisamy, K., Tsai, T. H., Yu, T. M., Sun, K. T., Yu, S. H., Lin, F. Y., ... & Li, C. Y. (2019). RNA‐binding protein, human antigen R regulates hypoxia‐induced autophagy by targeting ATG7/ATG16L1 expressions and autophagosome formation. Journal of cellular physiology, 234(5), 7448-7458.

Xia, L., Xu, J., Song, J., Xu, Y., Zhang, B., Gao, C., ... & Zhu, C. (2019). Autophagy-related gene 7 polymorphisms and cerebral palsy in Chinese infants. Frontiers in cellular neuroscience, 13, 494.

Yu, Y., Zhang, J., Jin, Y., Yang, Y., Shi, J., Chen, F., ... & Ni, X. (2018). MiR-20a-5p suppresses tumor proliferation by targeting autophagy-related gene 7 in neuroblastoma. Cancer cell international, 18(1), 1-10.

Yau, J. W., Singh, K. K., Hou, Y., Lei, X., Ramadan, A., Quan, A., ... & Verma, S. (2017). Endothelial-specific deletion of autophagy-related 7 (ATG7) attenuates arterial thrombosis in mice. The Journal of thoracic and cardiovascular surgery, 154(3), 978-988.

Su, L. Y., Luo, R., Liu, Q., Su, J. R., Yang, L. X., Ding, Y. Q., ... & Yao, Y. G. (2017). Atg5-and Atg7-dependent autophagy in dopaminergic neurons regulates cellular and behavioral responses to morphine. Autophagy, 13(9), 1496-1511.

Gong, C., Hu, C., Gu, F., Xia, Q., Yao, C., Zhang, L., ... & Gao, Y. (2017). Co-delivery of autophagy inhibitor ATG7 siRNA and docetaxel for breast cancer treatment. Journal of Controlled Release, 266, 272-286.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-ATG7 Recombinant Antibody (9H196)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare