Mouse Anti-ATOH1 Recombinant Antibody (18A6) (CBMAB-0876-YC)

Name: Mouse Anti-ATOH1 Recombinant Antibody (18A6)
SKU: CBMAB-0876-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ATOH1 Recombinant Antibody (18A6) (CBMAB-0876-YC) in WB

The expression of Atoh1 was assessed by western blot analysis. ...View More

Yu, X., Li, S., Xu, Y., Zhang, Y., Ma, W., Liang, C., ... & Li, J. (2020). Androgen maintains intestinal homeostasis by inhibiting BMP signaling via intestinal stromal cells. Stem Cell Reports, 15(4), 912-925.

Mouse Anti-ATOH1 Recombinant Antibody (18A6) (CBMAB-0876-YC) in IP

Co-immunoprecipitation (coIP) analysis of nuclear extracts from E17.5 cochleae or 293 cells cotransfected with indicated plasmids. Antibodies used for IP or for western detection are indicated. Anti-HA or -Flag was used for immunoprecipitating HA-Atoh1 or Flag-Pou4f3/Flag-Gfi1 fusion protein. ...View More

Li, J., Zhang, T., Ramakrishnan, A., Fritzsch, B., Xu, J., Wong, E. Y., ... & Xu, P. X. (2020). Dynamic changes in cis-regulatory occupancy by Six1 and its cooperative interactions with distinct cofactors drive lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium. Nucleic acids research, 48(6), 2880-2896.

Mouse Anti-ATOH1 Recombinant Antibody (18A6) (CBMAB-0876-YC) in IF

Representative images of Math1 staining in WT and VGFoverexpressing mice. Scale bar = 100 µm. ...View More

Mizoguchi, T., Shimazawa, M., Ohuchi, K., Kuse, Y., Nakamura, S., & Hara, H. (2019). Impaired cerebellar development in mice overexpressing VGF. Neurochemical Research, 44, 374-387.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

18A6

Application

IP, WB

Immunogen

Amino acids 2-189 of MATH-1 of human origin.

Specificity

Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATOH1

Introduction

ATOH1 (math1 (Atoh1)) belongs to the basic helix-loop-helix (BHLH) family of transcription factors, which activates E-box dependent transcription along with E47. ATOH1 is required for the formation of both neural and non-neural cell types. ATOH1 has been shown to be essential for formation of cerebellar granule neurons, inner ear hair cells, spinal cord interneurons, Merkel cells of the skin, and intestinal secretory cells.

Entrez Gene ID

11921

UniProt ID

P48985

Alternative Names

Ath1

Function

Transcriptional regulator. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. Plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription (By similarity).

Biological Process

Auditory receptor cell fate determination Source: Ensembl
Auditory receptor cell fate specification Source: Ensembl
Axon guidance Source: Ensembl
Central nervous system development Source: ProtInc
Cerebral cortex development Source: Ensembl
Inner ear morphogenesis Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Neuron differentiation Source: GO_Central
Neuron migration Source: Ensembl
Notch signaling pathway Source: Ensembl
Positive regulation of inner ear auditory receptor cell differentiation Source: Ensembl
Positive regulation of neuron differentiation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Transcription by RNA polymerase II Source: ProtInc

Cellular Location

Nucleus

Katsukura, N., Watanabe, S., Shirasaki, T., Hibiya, S., Kano, Y., Akahoshi, K., ... & Tsuchiya, K. (2021). Intestinal phenotype is maintained by Atoh1 in the cancer region of intraductal papillary mucinous neoplasm. Cancer science, 112(2), 932.

Fu, Y., Yuan, S. S., Zhang, L. J., Ji, Z. L., & Quan, X. J. (2020). Atonal bHLH transcription factor 1 is an important factor for maintaining the balance of cell proliferation and differentiation in tumorigenesis. Oncology Letters, 20(3), 2595-2605.

Cheng, Y. F. (2019). Atoh1 regulation in the cochlea: more than just transcription. Journal of Zhejiang University-SCIENCE B, 20(2), 146-155.

Xie, W. R., Jen, H. I., Seymour, M. L., Yeh, S. Y., Pereira, F. A., Groves, A. K., ... & Zoghbi, H. Y. (2017). An Atoh1-S193A phospho-mutant allele causes hearing deficits and motor impairment. Journal of Neuroscience, 37(36), 8583-8594.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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