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Mouse Anti-ATP6V0D1 Recombinant Antibody (2G12) (CBMAB-A4077-YC)

Provided herein is a Mouse monoclonal antibody against Human ATPase H+ Transporting V0 Subunit D1. The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all ATP6V0D1 antibodies
Published Data
Mouse Anti-ATP6V0D1 Recombinant Antibody (2G12) in WB
A stable reconstitution of pEGFP-p14 (p14rev) in p14 / rescued endogenous protein level of p14 as well as interacting partners MP1 and p18. Post-nuclear supernatants (PNS) and Crude Endocomes (CE) purified by subcellular fractionation were analyzed by Western blotting using specific antibodies against endosomal marker proteins. ...View More
Stasyk, T., Holzmann, J., Stumberger, S., Ebner, H. L., Hess, M. W., Bonn, G. K., ... & Huber, L. A. (2010). Proteomic analysis of endosomes from genetically modified p14/MP1 mouse embryonic fibroblasts. Proteomics, 10(22), 4117-4127.

Summary

Host Animal
Mouse
Specificity
Human
Clone
2G12
Antibody Isotype
IgG1, κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa238-309 from ATP6V0D1 (NP_004682) with GST tag.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Introduction
ATP6V0D1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, z
Entrez Gene ID
9114
UniProt ID
P61421
Alternative Names
ATPase H+ Transporting V0 Subunit D1; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump), Member D; ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D1; V-ATPase 40 KDa Accessory Protein; Vacuolar Proton Pump Subunit D 1; 32 KDa Accessory Prote
Function
Subunit of the integral membrane V0 complex of the lysosomal proton-transporting V-type ATPase (v-ATPase) (PubMed:28296633, PubMed:30374053).
V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (PubMed:30374053).
May play a role in coupling of proton transport and ATP hydrolysis (By similarity).
In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).
May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (By similarity).
Biological Process
Cellular iron ion homeostasis Source: UniProtKB
Cellular response to amino acid starvation Source: Reactome
Cellular response to increased oxygen levels Source: UniProtKB
Cilium assembly Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
IRE1-mediated unfolded protein response Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: UniProtKB
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Transferrin transport Source: Reactome
Vacuolar acidification Source: GO_Central
Vacuolar transport Source: GO_Central
Cellular Location
Lysosome membrane; Membrane. Localizes to centrosome and the base of the cilium.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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