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Mouse Anti-ATP6V1H Recombinant Antibody (EG310) (CBMAB-EN341-LY)

The product is antibody recognizes ATP6V1H . The antibody EG310 immunoassay techniques such as: IHC: 1:50~1:100 ELISA: 1:20000.
See all ATP6V1H antibodies
Published Data
Mouse Anti-ATP6V1H Recombinant Antibody (EG310) in Immunoblot
Control or Lat3 shRNA expressing differentiating MEL cells were metabolically labeled with or without L-AHA. Nascent proteins were visualized by streptavidin-HRP and other proteins were detected by immunoblotting. ...View More
Chung, J., Bauer, D. E., Ghamari, A., Nizzi, C. P., Deck, K. M., Kingsley, P. D., ... & Paw, B. H. (2015). The mTORC1/4E-BP pathway coordinates hemoglobin production with L-leucine availability. Science signaling, 8(372), ra34-ra34.
Mouse Anti-ATP6V1H Recombinant Antibody (EG310) in IF
Laser scanning confocal microscopy (LSCM) analysis of TM9SF4 (green) and ATP6V0A2 (red) in (a) SCR and (b) TM9SF4-KD HCT116 cells. Yellow/orange areas in merged picture indicate colocalization. Scale bar: 10 μm. ...View More
Lozupone, F., Borghi, M., Marzoli, F., Azzarito, T., Matarrese, P., Iessi, E., ... & Fais, S. (2015). TM9SF4 is a novel V-ATPase-interacting protein that modulates tumor pH alterations associated with drug resistance and invasiveness of colon cancer cells. Oncogene, 34(40), 5163-5174.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
EG310
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
Amino acids 184-483 mapping at the C-terminus of V-ATPase H of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ATPase H+ Transporting V1 Subunit H
Introduction
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Entrez Gene ID
Human525
Mouse110935
UniProt ID
HumanP15313
MouseQ66JS0
Alternative Names
ATPase H+ Transporting V1 Subunit H; ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H; Vacuolar Proton Pump Subunit SFD; Vacuolar ATP Synthase Subunit H; Vacuolar Proton Pump Subunit H; V-ATPase 50/57 KDa Subunits; Nef-Binding Protein 1; Protein VMA13 Homolog; V-ATPase Subunit H; NBP1;
Function
Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity).
Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.
Biological Process
Cellular response to amino acid starvation Source: Reactome
Endocytosis Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: UniProtKB
Regulation of defense response to virus by virus Source: Reactome
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Transferrin transport Source: Reactome
Vacuolar acidification Source: UniProtKB
Cellular Location
Cytosol; Extracellular exosome; Lysosomal membrane; Plasma membrane; Vacuolar proton-transporting V-type ATPase, V1 domain
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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