Mouse Anti-ATP6V1H Recombinant Antibody (EG310) (CBMAB-EN341-LY)

Name: Mouse Anti-ATP6V1H Recombinant Antibody (EG310)
SKU: CBMAB-EN341-LY
Rating: 5 (1 reviews)

See all ATP6V1H antibodies

Compare Online Inquiry

Published Data

Mouse Anti-ATP6V1H Recombinant Antibody (EG310) in Immunoblot

Control or Lat3 shRNA expressing differentiating MEL cells were metabolically labeled with or without L-AHA. Nascent proteins were visualized by streptavidin-HRP and other proteins were detected by immunoblotting. ...View More

Chung, J., Bauer, D. E., Ghamari, A., Nizzi, C. P., Deck, K. M., Kingsley, P. D., ... & Paw, B. H. (2015). The mTORC1/4E-BP pathway coordinates hemoglobin production with L-leucine availability. Science signaling, 8(372), ra34-ra34.

Mouse Anti-ATP6V1H Recombinant Antibody (EG310) in IF

Laser scanning confocal microscopy (LSCM) analysis of TM9SF4 (green) and ATP6V0A2 (red) in (a) SCR and (b) TM9SF4-KD HCT116 cells. Yellow/orange areas in merged picture indicate colocalization. Scale bar: 10 μm. ...View More

Lozupone, F., Borghi, M., Marzoli, F., Azzarito, T., Matarrese, P., Iessi, E., ... & Fais, S. (2015). TM9SF4 is a novel V-ATPase-interacting protein that modulates tumor pH alterations associated with drug resistance and invasiveness of colon cancer cells. Oncogene, 34(40), 5163-5174.

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG310

Application

ELISA, IF, IP, WB

Immunogen

Amino acids 184-483 mapping at the C-terminus of V-ATPase H of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ATPase H+ Transporting V1 Subunit H

Introduction

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Entrez Gene ID

Human	525
Mouse	110935

UniProt ID

Human	P15313
Mouse	Q66JS0

Alternative Names

ATPase H+ Transporting V1 Subunit H; ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H; Vacuolar Proton Pump Subunit SFD; Vacuolar ATP Synthase Subunit H; Vacuolar Proton Pump Subunit H; V-ATPase 50/57 KDa Subunits; Nef-Binding Protein 1; Protein VMA13 Homolog; V-ATPase Subunit H; NBP1;

Function

Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity).
Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.

Biological Process

Cellular response to amino acid starvation Source: Reactome
Endocytosis Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: UniProtKB
Regulation of defense response to virus by virus Source: Reactome
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Transferrin transport Source: Reactome
Vacuolar acidification Source: UniProtKB

Cellular Location

Cytosol; Extracellular exosome; Lysosomal membrane; Plasma membrane; Vacuolar proton-transporting V-type ATPase, V1 domain

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ATP6V1H Recombinant Antibody (EG310)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry