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Rabbit Anti-AXIN2 Recombinant Antibody (CBNH-009) (CBMAB-R4334-CN)

This product is a rabbit recombinant antibody that recognizes AXIN2. The antibody CBNH-009 can be used for immunoassay techniques such as: ELISA.
See all AXIN2 antibodies
Published Data
Rabbit Anti-AXIN2 Recombinant Antibody (CBNH-009) in WB
Expression analysis of Wnt-target proteins AXIN2, BMP4, CD44, CMYC, CYCLIN D1 and SURVIVIN after SFRP1 knockdown in RT12 and SFRP1 overexpression in BFTC905 using Western blotting analysis; AXIN2 and CD44 showed no protein expression, which was independent from SFRP1 expression; after semiquantitative analysis of protein bands, significant upregulation of CYCLIN D1 (126 %) and SURVIVIN (124 %) in RT112 with SFRP1-knockdown and significant downregulation of CMYC (80 %) and SURVIVN (63 %) in BFTC905 with SFRP1 overexpression could be observed. ...View More
Rogler, A., Kendziorra, E., Giedl, J., Stoehr, C., Taubert, H., Goebell, P. J., ... & Stoehr, R. (2015). Functional analyses and prognostic significance of SFRP1 expression in bladder cancer. Journal of cancer research and clinical oncology, 141, 1779-1790.

Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBNH-009
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Pro566 of human Axin2.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target

Full Name
axin 2
Introduction
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]
Entrez Gene ID
8313
UniProt ID
Q9Y2T1
Alternative Names
Axin 2; Conductin; Axil; Axis Inhibition Protein 2; Axin-Like Protein; ODCRCS; Axin-2;
Function
Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
Biological Process
Beta-catenin-TCF complex assembly Source: Reactome
Bone mineralization Source: Ensembl
Cell population proliferation Source: Ensembl
Cellular protein localization Source: BHF-UCL
Chondrocyte differentiation involved in endochondral bone morphogenesis Source: Ensembl
Intramembranous ossification Source: Ensembl
Maintenance of DNA repeat elements Source: BHF-UCL
mRNA stabilization Source: BHF-UCL
Negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
Negative regulation of cell population proliferation Source: BHF-UCL
Negative regulation of osteoblast differentiation Source: Ensembl
Odontogenesis Source: BHF-UCL
Positive regulation of canonical Wnt signaling pathway Source: Reactome
Positive regulation of cell death Source: BHF-UCL
Positive regulation of epithelial to mesenchymal transition Source: BHF-UCL
Positive regulation of protein phosphorylation Source: BHF-UCL
Regulation of centromeric sister chromatid cohesion Source: BHF-UCL
Regulation of chondrocyte development Source: Ensembl
Regulation of mismatch repair Source: BHF-UCL
Secondary heart field specification Source: Ensembl
Somitogenesis Source: Ensembl
Wnt signaling pathway Source: UniProtKB-KW
Cellular Location
Cytoplasm
Involvement in disease
Colorectal cancer (CRC): A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history.
Oligodontia-colorectal cancer syndrome (ODCRCS): Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.
PTM
Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.

Niu, Z., Wang, F., Lv, S., Lv, Y., Liu, M., Fu, L., ... & Yuan, F. (2021). HNRNPU-AS1 regulates cell proliferation and apoptosis via miR-205-5p/AXIN2 axis and Wnt/β-catenin signaling pathway in cervical cancer. Molecular and Cellular Biology, MCB-00115.

Li, X., Li, Y., Liu, G., & Wu, W. (2021). New insights of the correlation between AXIN2 polymorphism and cancer risk and susceptibility: evidence from 72 studies. BMC cancer, 21(1), 1-15.

Kaur, G., Verma, R., Mukhopadhyay, C. S., & Sethi, R. (2021). Elevated pulmonary levels of Axin2 in mice exposed to herbicide 2, 4‐D with or without endotoxin. Journal of Biochemical and Molecular Toxicology, e22912.

Moshkovsky, A. R., & Kirschner, M. W. (2021). The non-redundant nature of the Axin2 regulatory network in the canonical Wnt signaling pathway. bioRxiv.

Cavallo, J. C., Scholpp, S., & Flegg, M. B. (2020). Delay-driven oscillations via Axin2 feedback in the Wnt/β-catenin signalling pathway. Journal of Theoretical Biology, 507, 110458.

Bernkopf, D. B., Brückner, M., Hadjihannas, M. V., & Behrens, J. (2019). An aggregon in conductin/axin2 regulates Wnt/β-catenin signaling and holds potential for cancer therapy. Nature communications, 10(1), 1-14.

Chen, M., Liu, Y. Y., Zheng, M. Q., Wang, X. L., Gao, X. H., Chen, L., & Zhang, G. M. (2018). microRNA-544 promoted human osteosarcoma cell proliferation by downregulating AXIN2 expression. Oncology letters, 15(5), 7076-7082.

Haddaji Mastouri, M., De Coster, P., Zaghabani, A., Jammali, F., Raouahi, N., Ben Salem, A., ... & H'mida Ben Brahim, D. (2018). Genetic study of non‐syndromic tooth agenesis through the screening of paired box 9, msh homeobox 1, axin 2, and Wnt family member 10A genes: a case‐series. European journal of oral sciences, 126(1), 24-32.

Hloušková, A., Bielik, P., Bonczek, O., Balcar, V. J., & Šerý, O. (2017). Mutations in AXIN2 gene as a risk factor for tooth agenesis and cancer: A review. Neuroendocrinology Letters, 38(3).

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For research use only. Not intended for any clinical use.

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