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Rabbit Anti-AXL (Phosphorylated Tyr702) Recombinant Antibody (PTM020YC) (PTM-CBMAB-0059YC)

Provided herein is a Mouse monoclonal antibody against AXL Receptor Tyrosine Kinase. The antibody can be used for immunoassay techniques, such as WB, ICFC, ICC, CyTOF.
See all AXL antibodies
Published Data
Rabbit Anti-AXL (Phosphorylated Tyr702) Recombinant Antibody (PTM020YC) in IHC
786-O cells, 786-O cells that were stably infected with AXL or MET shRNA constructs were chronically treated with sunitinib (1 μM) and then deprived of sunitinib, and 786-O parental cells were seeded for wound-healing experiment as described in Methods. The gap distances in four independent experiments were measured and analyzed. ...View More
Zhou, L., Liu, X. D., Sun, M., Zhang, X., German, P., Bai, S., ... & Jonasch, E. (2016). Targeting MET and AXL overcomes resistance to sunitinib therapy in renal cell carcinoma. Oncogene, 35(21), 2687-2697.
Rabbit Anti-AXL (Phosphorylated Tyr702) Recombinant Antibody (PTM020YC) in WB
PRAME expression upon stimulation with 500 ng/mL Gas6 for 2 h in the absence (DMSO) or presence of 10 µM Axl inhibitor Cabozantinib (CABO) and upon administration of 900 ng/mL stimulatory Axl antibody (AF154) for 2 h. Protein levels were quantified by densitometry. ...View More
Hedrich, V., Breitenecker, K., Ortmayr, G., Pupp, F., Huber, H., Chen, D., ... & Mikulits, W. (2023). PRAME Is a Novel Target of Tumor-Intrinsic Gas6/Axl Activation and Promotes Cancer Cell Invasion in Hepatocellular Carcinoma. Cancers, 15(9), 2415.
Rabbit Anti-AXL (Phosphorylated Tyr702) Recombinant Antibody (PTM020YC) in WB
The C2C12 myotubes treated with 0.6 mM of palmitate (PA) with or without 100 nM insulin, then estimated p-IRS-1(307), p-Akt by western blotting. ...View More
Lee, C. H., Chiang, C. F., Lin, F. H., Kuo, F. C., Su, S. C., Huang, C. L., ... & Shieh, Y. S. (2022). PDIA4, a new endoplasmic reticulum stress protein, modulates insulin resistance and inflammation in skeletal muscle. Frontiers in endocrinology, 13, 1053882.

Summary

Host Animal
Rabbit
Specificity
Human
Clone
PTM020YC
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr702 of human Axl protein.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AXL Receptor Tyrosine Kinase
Introduction
AXL is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types.
Entrez Gene ID
558
UniProt ID
P30530
Alternative Names
AXL Receptor Tyrosine Kinase; AXL Oncogene; EC 2.7.10.1; UFO; Tyrosine-Protein Kinase Receptor UFO; AXL Transforming Sequence/Gene;
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.
(Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
(Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
Biological Process
Animal organ regeneration Source: Ensembl
Blood vessel remodeling Source: Ensembl
Cell maturation Source: UniProtKB
Cell migration Source: GO_Central
Cellular response to extracellular stimulus Source: Ensembl
Cellular response to hydrogen peroxide Source: Ensembl
Cellular response to interferon-alpha Source: UniProtKB
Cellular response to lipopolysaccharide Source: UniProtKB
Dendritic cell differentiation Source: UniProtKB
Erythrocyte homeostasis Source: Ensembl
Forebrain cell migration Source: Ensembl
Inflammatory response Source: Ensembl
Innate immune response Source: UniProtKB-KW
Multicellular organism development Source: GO_Central
Natural killer cell differentiation Source: GO_Central
Negative regulation of apoptotic process Source: GO_Central
Negative regulation of dendritic cell apoptotic process Source: UniProtKB
Negative regulation of interferon-gamma production Source: UniProtKB
Negative regulation of lymphocyte activation Source: Ensembl
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of tumor necrosis factor production Source: Ensembl
Nervous system development Source: GO_Central
Neuron migration Source: Ensembl
Neutrophil clearance Source: Ensembl
Ovulation cycle Source: Ensembl
Phagocytosis Source: UniProtKB
Platelet activation Source: GO_Central
Positive regulation of cytokine-mediated signaling pathway Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of natural killer cell differentiation Source: UniProtKB
Positive regulation of pinocytosis Source: CACAO
Positive regulation of protein kinase B signaling Source: GO_Central
Positive regulation of viral life cycle Source: FlyBase
Protein kinase B signaling Source: Ensembl
Secretion by cell Source: Ensembl
Signal transduction Source: ProtInc
Spermatogenesis Source: Ensembl
Substrate adhesion-dependent cell spreading Source: Ensembl
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Vagina development Source: Ensembl
Vascular endothelial growth factor receptor signaling pathway Source: Reactome
Viral entry into host cell Source: CACAO
Cellular Location
Cell membrane
Topology
Extracellular: 26-451 aa
Helical: 452-472 aa
Cytoplasmic: 473-894 aa
PTM
Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.

DeBerge, M., Glinton, K., Subramanian, M., Wilsbacher, L. D., Rothlin, C. V., Tabas, I., & Thorp, E. B. (2021). Macrophage AXL receptor tyrosine kinase inflames the heart after reperfused myocardial infarction. The Journal of clinical investigation, 131(6).

Brenig, R., Pop, O. T., Triantafyllou, E., Geng, A., Singanayagam, A., Perez-Shibayama, C., ... & Bernsmeier, C. (2020). Expression of AXL receptor tyrosine kinase relates to monocyte dysfunction and severity of cirrhosis. Life science alliance, 3(1).

Namiki, K., Wongsirisin, P., Yokoyama, S., Sato, M., Rawangkan, A., Sakai, R., ... & Suganuma, M. (2020). (−)-Epigallocatechin gallate inhibits stemness and tumourigenicity stimulated by AXL receptor tyrosine kinase in human lung cancer cells. Scientific reports, 10(1), 1-11.

Lauter, M., Weber, A., & Torka, R. (2019). Targeting of the AXL receptor tyrosine kinase by small molecule inhibitor leads to AXL cell surface accumulation by impairing the ubiquitin-dependent receptor degradation. Cell Communication and Signaling, 17(1), 1-14.

Yan, S., Vandewalle, N., De Beule, N., Faict, S., Maes, K., De Bruyne, E., ... & De Veirman, K. (2019). AXL receptor tyrosine kinase as a therapeutic target in hematological malignancies: focus on multiple myeloma. Cancers, 11(11), 1727.

Zhang, G., Wang, M., Zhao, H., & Cui, W. (2018). Function of Axl receptor tyrosine kinase in non-small cell lung cancer. Oncology letters, 15(3), 2726-2734.

Badi, I., Mancinelli, L., Polizzotto, A., Ferri, D., Zeni, F., Burba, I., ... & Raucci, A. (2018). miR-34a promotes vascular smooth muscle cell calcification by downregulating SIRT1 (Sirtuin 1) and Axl (AXL Receptor Tyrosine Kinase). Arteriosclerosis, thrombosis, and vascular biology, 38(9), 2079-2090.

Zhen, Y., Lee, I. J., Finkelman, F. D., & Shao, W. H. (2018). Targeted inhibition of Axl receptor tyrosine kinase ameliorates anti-GBM-induced lupus-like nephritis. Journal of autoimmunity, 93, 37-44.

Kanlikilicer, P., Ozpolat, B., Aslan, B., Bayraktar, R., Gurbuz, N., Rodriguez-Aguayo, C., ... & Lopez-Berestein, G. (2017). Therapeutic targeting of AXL receptor tyrosine kinase inhibits tumor growth and intraperitoneal metastasis in ovarian cancer models. Molecular Therapy-Nucleic Acids, 9, 251-262.

Aguilera, T. A., & Giaccia, A. J. (2017). Molecular pathways: oncologic pathways and their role in T-cell exclusion and immune evasion—a new role for the AXL receptor tyrosine kinase. Clinical Cancer Research, 23(12), 2928-2933.

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For research use only. Not intended for any clinical use.

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