Mouse Anti-BarX2 Recombinant Antibody (8A7/1) (CBMAB-XB0080-YC)

Name: Mouse Anti-BarX2 Recombinant Antibody (8A7/1)
SKU: CBMAB-XB0080-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-BarX2 Recombinant Antibody (8A7/1) in WB

Protein expression of BARX2, KRT16, pMEK1, MEK1, p-ERK1/2 and ERK1/2 in C666-1 and HNE3 cells examined using Western blot. ...View More

Lu, Z., Peng, H., Li, R., Xu, X., & Peng, J. (2022). BarH-like homeobox 2 represses the transcription of keratin 16 and affects Ras signaling pathway to suppress nasopharyngeal carcinoma progression. Bioengineered, 13(2), 3122-3136.

Mouse Anti-BarX2 Recombinant Antibody (8A7/1) in IHC

BARX2 expression in normal gastric mucosa and at various pathological stages of gastric adenocarcinoma. (A-G) Representative immunohistochemical staining images of BARX2 expression in normal gastric mucosa (A) and at various pathological stages of gastric adenocarcinoma: T1N0M0 (Ia) (B), T2N0M0 (Ib) (C), T3N0M0 (II) (D), T3N1M0 (IIIa) (E), T4N1M0 (IIIb) (F) and T4N3M1 (IV) (G). (H) BARX2 expression was progressively decreased from TNM stage I to IV in the gastric adenocarcinoma tissues (P<0.001). ...View More

Ma, J., Xia, L. L., Yao, X. Q., Zheng, S. M., Li, S., Xu, L. S., ... & Li, Z. S. (2020). BARX2 expression is downregulated by CpG island hypermethylation and is associated with suppressed cell proliferation and invasion of gastric cancer cells. Oncology Reports, 43(6), 1805-1818.

Mouse Anti-BarX2 Recombinant Antibody (8A7/1) in WB

All xenografts were collected for western blot analysis. Upregulation of BARX2 suppressed expression of Ki-67, PCNA and MMPs (MMP3, MMP7 and MMP9), while upregulation of BARX2 stimulated the expression of
E-cadherin in the tumor tissues. Upregulation of BARX2 also stimulated the expression of Bcl-2 but suppressed the expression of Bax and caspase-9 in the tumor tissues. *P<0.05, **P<0.01, ***P<0.001, compared with AGS-LV. ...View More

Datasheet

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Basic Information

Host Animal

Mouse

Clone

8A7/1

Application

WB, IF, IP, IHC-P

Immunogen

MBP-tagged BarX2.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BARX Homeobox 2

Introduction

BarX2 is a member of the homeobox transcription factor family. A highly related protein in mouse has been shown to influence cellular processes that control cell adhesion and remodeling of the actin cytoskeleton in myoblast fusion and chondrogenesis. The encoded protein may also play a role in cancer progression.

Entrez Gene ID

8538

UniProt ID

Q9UMQ3

Alternative Names

BARX Homeobox 2; BarH-Like Homeobox 2;

Function

Transcription factor. Binds optimally to the DNA consensus sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural adhesion molecules such as L1 or Ng-CAM during embryonic development of both the central and peripherical nervous system. May be involved in controlling adhesive processes in keratinizing epithelia (By similarity).

Biological Process

Cartilage condensation Source: Ensembl
Myotube differentiation Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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