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Mouse Anti-BarX2 Recombinant Antibody (8A7/1) (CBMAB-XB0080-YC)

Provided herein is a Mouse Recombinant Antibody against BARX Homeobox 2. The antibody can be used for immunoassay techniques, such as ELISA, IHC, IF, WB.
See all BarX2 antibodies
Published Data
Mouse Anti-BarX2 Recombinant Antibody (8A7/1) in WB
Protein expression of BARX2, KRT16, pMEK1, MEK1, p-ERK1/2 and ERK1/2 in C666-1 and HNE3 cells examined using Western blot. ...View More
Lu, Z., Peng, H., Li, R., Xu, X., & Peng, J. (2022). BarH-like homeobox 2 represses the transcription of keratin 16 and affects Ras signaling pathway to suppress nasopharyngeal carcinoma progression. Bioengineered, 13(2), 3122-3136.
Mouse Anti-BarX2 Recombinant Antibody (8A7/1) in IHC
BARX2 expression in normal gastric mucosa and at various pathological stages of gastric adenocarcinoma. (A-G) Representative immunohistochemical staining images of BARX2 expression in normal gastric mucosa (A) and at various pathological stages of gastric adenocarcinoma: T1N0M0 (Ia) (B), T2N0M0 (Ib) (C), T3N0M0 (II) (D), T3N1M0 (IIIa) (E), T4N1M0 (IIIb) (F) and T4N3M1 (IV) (G). (H) BARX2 expression was progressively decreased from TNM stage I to IV in the gastric adenocarcinoma tissues (P<0.001). ...View More
Ma, J., Xia, L. L., Yao, X. Q., Zheng, S. M., Li, S., Xu, L. S., ... & Li, Z. S. (2020). BARX2 expression is downregulated by CpG island hypermethylation and is associated with suppressed cell proliferation and invasion of gastric cancer cells. Oncology Reports, 43(6), 1805-1818.
Mouse Anti-BarX2 Recombinant Antibody (8A7/1) in WB
All xenografts were collected for western blot analysis. Upregulation of BARX2 suppressed expression of Ki-67, PCNA and MMPs (MMP3, MMP7 and MMP9), while upregulation of BARX2 stimulated the expression of
E-cadherin in the tumor tissues. Upregulation of BARX2 also stimulated the expression of Bcl-2 but suppressed the expression of Bax and caspase-9 in the tumor tissues. *P<0.05, **P<0.01, ***P<0.001, compared with AGS-LV. ...View More
Ma, J., Xia, L. L., Yao, X. Q., Zheng, S. M., Li, S., Xu, L. S., ... & Li, Z. S. (2020). BARX2 expression is downregulated by CpG island hypermethylation and is associated with suppressed cell proliferation and invasion of gastric cancer cells. Oncology Reports, 43(6), 1805-1818.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
8A7/1
Antibody Isotype
IgG1, κ
Application
WB, IF, IP, IHC-P

Basic Information

Immunogen
MBP-tagged BarX2.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BARX Homeobox 2
Introduction
BarX2 is a member of the homeobox transcription factor family. A highly related protein in mouse has been shown to influence cellular processes that control cell adhesion and remodeling of the actin cytoskeleton in myoblast fusion and chondrogenesis. The encoded protein may also play a role in cancer progression.
Entrez Gene ID
8538
UniProt ID
Q9UMQ3
Alternative Names
BARX Homeobox 2; BarH-Like Homeobox 2;
Function
Transcription factor. Binds optimally to the DNA consensus sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural adhesion molecules such as L1 or Ng-CAM during embryonic development of both the central and peripherical nervous system. May be involved in controlling adhesive processes in keratinizing epithelia (By similarity).
Biological Process
Cartilage condensation Source: Ensembl
Myotube differentiation Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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