Mouse Anti-BGN Recombinant Antibody (4E1-1G7) (CBMAB-P0101-YC)

Name: Mouse Anti-BGN Recombinant Antibody (4E1-1G7)
SKU: CBMAB-P0101-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-BGN Recombinant Antibody (4E1-1G7) (CBMAB-P0101-YC) in IHC

Photomicrographs of BGN protein expression in (A) invasive ductal carcinoma, and in (B) normal breast tissue. Lung tissue was used as an external negative (C) and positive (D) control. Bars represent 50 μm. Scatter dot plot (E) demonstrates a significant reduction of BGN protein expression in breast cancer tissue, compared to benign breast tissue. ...View More

Thiesen, A. P., Mielczarski, B., & Savaris, R. F. (2023). Deep learning neural network image analysis of immunohistochemical protein expression reveals a significantly reduced expression of biglycan in breast cancer. PloS one, 18(3), e0282176.

Mouse Anti-BGN Recombinant Antibody (4E1-1G7) (CBMAB-P0101-YC) in IHC

Example of BGN expression in DAB only in photomicrographs, after color deconvolution in normal breast tissue (A) and with cancer (B), bars represent 50 μm. ...View More

Mouse Anti-BGN Recombinant Antibody (4E1-1G7) (CBMAB-P0101-YC) in IHC and Immunoblot

BGN protein in CADASIL and control brain. Immunohistochemical analysis of geneticallydefined CADASIL brains (A and B) and matched controls (C) was performed on frontal cortex sections with antibody 4E1-1G7. Penetrating arteries (A, C) and leptomeningeal (B) vessels are shown. Magnification is at 400x. (D) Brain lysates were analyzed by immunoblotting with 4E1-1G7 and the core BGN protein band was quantified for
CADASIL and control frontal cortex, normalized to tubulin levels. Protein was not treated with glycosidases. Difference were significant (p<0.05) for CADASIL samples versus controls. ...View More

Zhang, X., Lee, S. J., Young, M. F., & Wang, M. M. (2015). The small leucine-rich proteoglycan BGN accumulates in CADASIL and binds to NOTCH3. Translational stroke research, 6, 148-155.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4E1-1G7

Application

WB, IF, IP, ELISA

Immunogen

Recombinant protein corresponding to aa 1-369 of human Proteoglycan 1.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	3 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

biglycan

Introduction

BGN is a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in bone growth, muscle development and regeneration, and collagen fibril assembly in multiple tissues. This protein may also regulate inflammation and innate immunity. Additionally, the encoded protein may contribute to atherosclerosis and aortic valve stenosis in human patients. This gene and the related gene decorin are thought to be the result of a gene duplication.

Entrez Gene ID

633

UniProt ID

P21810

Alternative Names

SLRR1A; MRLS; PGI; SEMDX; DSPG1; PG-S1

Function

May be involved in collagen fiber assembly.

Biological Process

Articular cartilage development Source: Ensembl
Blood vessel remodeling Source: Ensembl
Bone development Source: Ensembl
Chondroitin sulfate biosynthetic process Source: Reactome
Chondroitin sulfate catabolic process Source: Reactome
Dermatan sulfate biosynthetic process Source: Reactome
Extracellular matrix organization Source: Reactome
Peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan Source: Ensembl

Cellular Location

Extracellular matrix

Involvement in disease

Meester-Loeys syndrome (MRLS): An X-linked, thoracic aortic aneurysm syndrome characterized by early-onset, severe aortic aneurysm and dissection. Other recurrent findings include hypertelorism, pectus deformity, joint hypermobility, contractures, and mild skeletal dysplasia.
Spondyloepimetaphyseal dysplasia, X-linked (SEMDX): An X-linked recessive bone disease characterized by severe short-trunk dwarfism, brachydactyly, metaphyseal flaring of lower extremities, short and broad long bone diaphyses, moderate platyspondyly, normal facies, and normal intelligence.

PTM

The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.

Shao, C., Cheng, C., Shao, Q., & Chen, B. (2021). Identification and Validation of Biglycan as Prognosis and Therapy Markers for Patients with Stomach Adenocarcinoma. International Journal of General Medicine, 14, 3497.

Salimi, H., Suzuki, A., Habibi, H., Orita, K., Hori, Y., Yabu, A., ... & Nakamura, H. (2021). Biglycan expression and its function in human ligamentum flavum. Scientific reports, 11(1), 1-13.

Hua, R., Ni, Q., Eliason, T. D., Han, Y., Gu, S., Nicolella, D. P., ... & Jiang, J. X. (2020). Biglycan and chondroitin sulfate play pivotal roles in bone toughness via retaining bound water in bone mineral matrix. Matrix Biology, 94, 95-109.

Scuruchi, M., Potì, F., Rodríguez-Carrio, J., Campo, G. M., & Mandraffino, G. (2020). Biglycan and atherosclerosis: Lessons from high cardiovascular risk conditions. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1865(2), 158545.

Zhao, S. F., Yin, X. J., Zhao, W. J., Liu, L. C., & Wang, Z. P. (2020). Biglycan as a potential diagnostic and prognostic biomarker in multiple human cancers. Oncology letters, 19(3), 1673-1682.

Schulz, G. B., Grimm, T., Sers, C., Riemer, P., Elmasry, M., Kirchner, T., ... & Horst, D. (2019, August). Prognostic value and association with epithelial-mesenchymal transition and molecular subtypes of the proteoglycan biglycan in advanced bladder cancer. In Urologic Oncology: Seminars and Original Investigations (Vol. 37, No. 8, pp. 530-e9). Elsevier.

Zhang, Y. J., Qing, Q., Zhang, Y. J., Ning, L. J., Cui, J., Yao, X., ... & Qin, T. W. (2019). Enhancement of tenogenic differentiation of rat tendon‐derived stem cells by biglycan. Journal of cellular physiology, 234(9), 15898-15910.

Guo, D., Zhang, W., Yang, H., Bi, J., Xie, Y., Cheng, B., ... & Chen, S. (2019). Celastrol induces necroptosis and ameliorates inflammation via targeting biglycan in human gastric carcinoma. International journal of molecular sciences, 20(22), 5716.

Liu, B., Xu, T., Xu, X., Cui, Y., & Xing, X. (2018). Biglycan promotes the chemotherapy resistance of colon cancer by activating NF-κB signal transduction. Molecular and cellular biochemistry, 449(1), 285-294.

Jacobsen, F., Kraft, J., Schroeder, C., Hube-Magg, C., Kluth, M., Lang, D. S., ... & Melling, N. (2017). Up-regulation of biglycan is associated with poor prognosis and PTEN deletion in patients with prostate cancer. Neoplasia, 19(9), 707-715.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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