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Mouse Anti-BMP2 Recombinant Antibody (4B12) (CBMAB-A0809-LY)

The product is antibody recognizes BMP2. The antibody 4B12 immunoassay techniques such as: WB, ELISA.
See all BMP2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4B12
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
BMP2 (NP_001191, 283 a.a.-396 a.a.) partial recombinant protein.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Bone Morphogenetic Protein 2
Introduction
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq]
Entrez Gene ID
UniProt ID
Alternative Names
BMP2A
Function
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:31019025, PubMed:24362451).
Induces cartilage and bone formation (PubMed:3201241).
Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533).
Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754).
In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:20851880).
Stimulates also the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451).
Biological Process
Activation of MAPK activity Source: AgBase
Animal organ morphogenesis Source: UniProtKB
Aortic valve development Source: BHF-UCL
Atrioventricular canal morphogenesis Source: BHF-UCL
Atrioventricular valve morphogenesis Source: UniProtKB
BMP signaling pathway Source: BHF-UCL
BMP signaling pathway involved in heart development Source: BHF-UCL
BMP signaling pathway involved in heart induction Source: BHF-UCL
Bone mineralization Source: UniProtKB
Bone mineralization involved in bone maturation Source: BHF-UCL
Branching involved in ureteric bud morphogenesis Source: UniProtKB
Cardiac atrium formation Source: BHF-UCL
Cardiac epithelial to mesenchymal transition Source: BHF-UCL
Cardiac jelly development Source: BHF-UCL
Cardiac muscle cell differentiation Source: BHF-UCL
Cardiac muscle tissue morphogenesis Source: UniProtKB
Cardiocyte differentiation Source: MGI
Cell-cell signaling Source: ProtInc
Cell fate commitment Source: UniProtKB
Cellular response to BMP stimulus Source: BHF-UCL
Cellular response to organic cyclic compound Source: Ensembl
Chondrocyte differentiation Source: AgBase
Corticotropin hormone secreting cell differentiation Source: UniProtKB
Embryonic heart tube anterior/posterior pattern specification Source: UniProtKB
Endocardial cushion formation Source: BHF-UCL
Endocardial cushion morphogenesis Source: UniProtKB
Epithelial to mesenchymal transition Source: BHF-UCL
Heart development Source: UniProtKB
Inflammatory response Source: UniProtKB
Inner ear development Source: UniProtKB
In utero embryonic development Source: UniProtKB
Mesenchymal cell differentiation Source: UniProtKB
Mesenchymal cell proliferation involved in ureteric bud development Source: UniProtKB
Mesenchyme development Source: BHF-UCL
Negative regulation of aldosterone biosynthetic process Source: BHF-UCL
Negative regulation of BMP signaling pathway Source: Reactome
Negative regulation of calcium-independent cell-cell adhesion Source: AgBase
Negative regulation of canonical Wnt signaling pathway Source: AgBase
Negative regulation of cardiac muscle cell differentiation Source: BHF-UCL
Negative regulation of cell cycle Source: HGNC-UCL
Negative regulation of cell population proliferation Source: BHF-UCL
Negative regulation of cortisol biosynthetic process Source: BHF-UCL
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of insulin-like growth factor receptor signaling pathway Source: BHF-UCL
Negative regulation of steroid biosynthetic process Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Negative regulation of Wnt signaling pathway involved in heart development Source: BHF-UCL
Notch signaling pathway Source: UniProtKB
Odontogenesis of dentin-containing tooth Source: UniProtKB
Osteoblast differentiation Source: MGI
Pathway-restricted SMAD protein phosphorylation Source: BHF-UCL
Pericardium development Source: UniProtKB
Positive regulation of apoptotic process Source: MGI
Positive regulation of astrocyte differentiation Source: UniProtKB
Positive regulation of bone mineralization Source: BHF-UCL
Positive regulation of cartilage development Source: MGI
Positive regulation of cell migration Source: UniProtKB
Positive regulation of endothelial cell proliferation Source: UniProtKB
Positive regulation of epithelial to mesenchymal transition Source: BHF-UCL
Positive regulation of ERK1 and ERK2 cascade Source: DFLAT
Positive regulation of extracellular matrix constituent secretion Source: BHF-UCL
Positive regulation of fat cell differentiation Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of MAPK cascade Source: DFLAT
Positive regulation of neuron differentiation Source: UniProtKB
Positive regulation of odontogenesis Source: UniProtKB
Positive regulation of ossification Source: MGI
Positive regulation of osteoblast differentiation Source: BHF-UCL
Positive regulation of osteoblast proliferation Source: UniProtKB
Positive regulation of p38MAPK cascade Source: DFLAT
Positive regulation of pathway-restricted SMAD protein phosphorylation Source: UniProtKB
Positive regulation of phosphatase activity Source: MGI
Positive regulation of pri-miRNA transcription by RNA polymerase II Source: Ensembl
Positive regulation of protein binding Source: BHF-UCL
Positive regulation of protein phosphorylation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
Positive regulation of Wnt signaling pathway Source: UniProtKB
Positive regulation of Wnt signaling pathway by BMP signaling pathway Source: UniProtKB
Protein destabilization Source: Ensembl
Protein phosphorylation Source: DFLAT
Proteoglycan metabolic process Source: Ensembl
Regulation of odontogenesis of dentin-containing tooth Source: Ensembl
Regulation of transcription, DNA-templated Source: HGNC-UCL
Response to bacterium Source: Ensembl
Response to hypoxia Source: UniProtKB
Sequestering of BMP from receptor via BMP binding Source: Reactome
Skeletal system development Source: ProtInc
SMAD protein signal transduction Source: UniProtKB
Telencephalon development Source: MGI
Telencephalon regionalization Source: UniProtKB
Thyroid-stimulating hormone-secreting cell differentiation Source: UniProtKB
Cellular Location
Secreted
Involvement in disease
Brachydactyly A2 (BDA2): The gene represented in this entry is involved in disease pathogenesis. Duplications of a cis-regulatory element located approximately 110 kb downstream of BMP2 have been found in BDA2 families. They likely cause altered BMP2 expression with pathological consequences. A form of brachydactyly. Brachydactyly defines a group of inherited malformations characterized by shortening of the digits due to abnormal development of the phalanges and/or the metacarpals. In brachydactyly type A2 shortening of the middle phalanges is confined to the index finger and the second toe, all other digits being more or less normal. Because of a rhomboid or triangular shape of the affected middle phalanx, the end of the second finger usually deviates radially.
Short stature, facial dysmorphism, and skeletal anomalies with or without cardiac anomalies (SSFSC): An autosomal dominant disorder characterized by short stature, facial dysmorphism, skeletal anomalies, and variable cardiac defects. Distinctive facial features include midface retrusion, short upturned nose, long philtrum, high-arched or cleft palate, and variable degrees of micrognathia and dental crowding. Skeletal anomalies include patterning defects of the axial skeleton, characterized by 11 pairs of ribs and brachydactyly of the fifth ray. Congenital heart defects are variably observed and appear to involve primarily the cardiac outflow tract.

Cha, J. K., Song, Y. W., Kim, S., Thoma, D. S., Jung, U. W., & Jung, R. E. (2021). Core Ossification of Bone Morphogenetic Protein-2-Loaded Collagenated Bone Mineral in the Sinus. Tissue Engineering Part A, 27(13-14), 905-913.

Sun, P., Shi, A., Shen, C., Liu, Y., Wu, G., & Feng, J. (2020). Human salivary histatin‐1 (Hst1) promotes bone morphogenetic protein 2 (BMP2)‐induced osteogenesis and angiogenesis. FEBS Open bio, 10(8), 1503-1515.

Hettiaratchi, M. H., Krishnan, L., Rouse, T., Chou, C., McDevitt, T. C., & Guldberg, R. E. (2020). Heparin-mediated delivery of bone morphogenetic protein-2 improves spatial localization of bone regeneration. Science advances, 6(1), eaay1240.

Xiao, X., Dev, S., Canali, S., Bayer, A., Xu, Y., Agarwal, A., ... & Babitt, J. L. (2020). Endothelial bone morphogenetic protein 2 (Bmp2) knockout exacerbates hemochromatosis in homeostatic iron regulator (Hfe) knockout mice but not bmp6 knockout mice. Hepatology, 72(2), 642-655.

Martini, F., Pellati, A., Mazzoni, E., Salati, S., Caruso, G., Contartese, D., & De Mattei, M. (2020). Bone morphogenetic protein-2 signaling in the osteogenic differentiation of human bone marrow mesenchymal stem cells induced by pulsed electromagnetic fields. International journal of molecular sciences, 21(6), 2104.

Halloran, D., Durbano, H. W., & Nohe, A. (2020). Bone morphogenetic protein-2 in development and bone homeostasis. Journal of Developmental Biology, 8(3), 19.

Pearson, H. B., Mason, D. E., Kegelman, C. D., Zhao, L., Dawahare, J. H., Kacena, M. A., & Boerckel, J. D. (2019). Effects of bone morphogenetic protein-2 on neovascularization during large bone defect regeneration. Tissue Engineering Part A, 25(23-24), 1623-1634.

Anouz, R., Repanas, A., Schwarz, E., & Groth, T. (2018). Novel Surface Coatings Using Oxidized Glycosaminoglycans as Delivery Systems of Bone Morphogenetic Protein 2 (BMP‐2) for Bone Regeneration. Macromolecular bioscience, 18(11), 1800283.

Olthof, M. G., Kempen, D. H., Liu, X., Dadsetan, M., Tryfonidou, M. A., Yaszemski, M. J., ... & Lu, L. (2018). Bone morphogenetic protein‐2 release profile modulates bone formation in phosphorylated hydrogel. Journal of tissue engineering and regenerative medicine, 12(6), 1339-1351.

Dube, P. R., Birnbaumer, L., & Vazquez, G. (2017). Evidence for constitutive bone morphogenetic protein-2 secretion by M1 macrophages: Constitutive auto/paracrine osteogenic signaling by BMP-2 in M1 macrophages. Biochemical and biophysical research communications, 491(1), 154-158.

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For research use only. Not intended for any clinical use.

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