Sign in or Register   Sign in or Register
  |  

Mouse Anti-C5AR1 Recombinant Antibody (3H1740) (CBMAB-C1187-CN)

This product is a Mouse antibody that recognizes C5AR1. The antibody 3H1740 can be used for immunoassay techniques such as: FC, IHC, WB.
See all C5AR1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Monkey
Clone
3H1740
Antibody Isotype
IgG2a
Application
FC, IHC, WB

Basic Information

Immunogen
C5aR-peptide: Met1-Asn31
Specificity
Human, Monkey
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Concentration
1 mg/mL

Target

Full Name
Complement C5a Receptor 1
Introduction
C5AR1 (Complement C5a Receptor 1) is a Protein Coding gene. Diseases associated with C5AR1 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus. Among its related pathways are Creation of C4 and C2 activators and Complement and coagulation cascades. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and complement component C5a binding. An important paralog of this gene is C5AR2.
Entrez Gene ID
Human728
Monkey718056
UniProt ID
HumanP21730
MonkeyP79188
Alternative Names
Complement C5a Receptor 1; Complement Component 5a Receptor 1; C5a-R; C5R1; C5AR; Complement Component 5 Receptor 1 (C5a Ligand); C5a Anaphylatoxin Chemotactic Receptor 1; C5a Anaphylatoxin Chemotactic Receptor;
Function
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed:1847994, PubMed:8182049, PubMed:7622471, PubMed:9553099, PubMed:10636859, PubMed:15153520, PubMed:29300009).
The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed:8182049, PubMed:7622471, PubMed:9553099).
Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed:10636859, PubMed:15153520).
Biological Process
Activation of MAPK activity Source: ProtInc
Activation of phospholipase C activity Source: ProtInc
Amyloid-beta clearance Source: ARUK-UCL
Animal organ regeneration Source: Ensembl
Apoptotic process Source: Ensembl
Astrocyte activation Source: ARUK-UCL
Cell proliferation in hindbrain Source: Ensembl
Cellular defense response Source: ProtInc
Chemotaxis Source: ProtInc
Cognition Source: ARUK-UCL
Complement component C5a signaling pathway Source: UniProtKB
Complement receptor mediated signaling pathway Source: GO_Central
Defense response to Gram-positive bacterium Source: Ensembl
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: ProtInc
Inflammatory response Source: GO_Central
Microglial cell activation Source: ARUK-UCL
mRNA transcription by RNA polymerase II Source: UniProtKB
Negative regulation of neuron apoptotic process Source: Ensembl
Neutrophil chemotaxis Source: Ensembl
Neutrophil degranulation Source: Reactome
Phospholipase C-activating G protein-coupled receptor signaling pathway Source: GO_Central
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of epithelial cell proliferation Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of macrophage chemotaxis Source: Ensembl
Positive regulation of neutrophil chemotaxis Source: Ensembl
Positive regulation of vascular endothelial growth factor production Source: Ensembl
Presynapse organization Source: ARUK-UCL
Regulation of complement activation Source: Reactome
Regulation of tau-protein kinase activity Source: ARUK-UCL
Response to lipopolysaccharide Source: Ensembl
Response to peptidoglycan Source: Ensembl
Sensory perception of chemical stimulus Source: ProtInc
Signal transduction Source: ProtInc
Cellular Location
Cell membrane; Cytoplasmic vesicle. Phosphorylated C5aR colocalizes with ARRB1 and ARRB2 in cytoplasmic vesicles.
Topology
Extracellular: 1-37 aa
Helical: 38-64 aa
Cytoplasmic: 65-69 aa
Helical: 70-93 aa
Extracellular: 94-110 aa
Helical: 111-132 aa
Cytoplasmic: 133-153 aa
Helical: 154-174 aa
Extracellular: 175-200 aa
Helical: 201-226 aa
Cytoplasmic: 227-242 aa
Helical: 243-265 aa
Extracellular: 266-282 aa
Helical: 283-303 aa
Cytoplasmic: 304-350 aa
PTM
Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist (PubMed:11342590). Sulfation at Tyr-14 is important for CHIPS binding (PubMed:21706042).
Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338.

Nürge, B., Schulz, A. L., Kaemmerer, D., Sänger, J., Evert, K., Schulz, S., & Lupp, A. (2021). Immunohistochemical identification of complement peptide C5a receptor 1 (C5aR1) in non-neoplastic and neoplastic human tissues. PloS one, 16(2), e0246939.

Nording, H., Baron, L., Haberthür, D., Emschermann, F., Mezger, M., Sauter, M., ... & Langer, H. F. (2021). The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets. Nature communications, 12(1), 1-22.

Wu, M. C., Lee, J. D., Ruitenberg, M. J., & Woodruff, T. M. (2020). Absence of the C5a Receptor C5aR2 Worsens Ischemic Tissue Injury by Increasing C5aR1-Mediated Neutrophil Infiltration. The Journal of Immunology, 205(10), 2834-2839.

Song, N., Li, P., Jiang, Y., Sun, H., Cui, J., Zhao, G., ... & Zhou, Y. (2018). C5a receptor1 inhibition alleviates influenza virus-induced acute lung injury. International immunopharmacology, 59, 12-20.

Herrmann, J. B., Muenstermann, M., Strobel, L., Schubert-Unkmeir, A., Woodruff, T. M., Gray-Owen, S. D., ... & Johswich, K. O. (2018). Complement C5a receptor 1 exacerbates the pathophysiology of N. meningitidis sepsis and is a potential target for disease treatment. MBio, 9(1), e01755-17.

Dick, J., Gan, P. Y., Ford, S. L., Odobasic, D., Alikhan, M. A., Loosen, S. H., ... & Holdsworth, S. R. (2018). C5a receptor 1 promotes autoimmunity, neutrophil dysfunction and injury in experimental anti-myeloperoxidase glomerulonephritis. Kidney international, 93(3), 615-625.

Ender, F., Wiese, A. V., Schmudde, I., Sun, J., Vollbrandt, T., König, P., ... & Köhl, J. (2017). Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase. PLoS One, 12(2), e0172446.

Ask a question We look forward to hearing from you.
0 reviews or Q&As
Loading...
Have you used Mouse Anti-C5AR1 Recombinant Antibody (3H1740)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare