Mouse Anti-CACNA2D1 Recombinant Antibody (CBFYC-0720) (CBMAB-C0775-FY)

Name: Mouse Anti-CACNA2D1 Recombinant Antibody (CBFYC-0720)
SKU: CBMAB-C0775-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CACNA2D1 Recombinant Antibody (CBFYC-0720) (CBMAB-C0775-FY) in Immunoblot

A and B, co-IP analysis shows the protein-protein interaction between α2δ-1 and GluN1 in the membrane extracts from striatal tissues of mice (A) and humans (B, two separate subjects). The proteins were immunoprecipitated initially with a mouse anti-α2δ-1 antibody or IgG. Western immunoblotting was performed using a rabbit anti-GluN1 or a rabbit anti-α2δ-1 antibody. IgG and input (tissue lysates only, without immunoprecipitation) were used as negative and positive controls. Similar data were obtained from striatal tissues from each of the four human donors. ...View More

Zhou, J. J., Li, D. P., Chen, S. R., Luo, Y., & Pan, H. L. (2018). The α2δ-1-NMDA receptor coupling is essential for corticostriatal long-term potentiation and is involved in learning and memory. Journal of Biological Chemistry, 293(50), 19354-19364.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0720

Application

WB, IP, IF, E, P

Immunogen

Amino acids 25-171 mapping near the N-terminus of Cacna2d1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CALCIUM VOLTAGE-GATED CHANNEL AUXILIARY SUBUNIT ALPHA2DELTA 1

Introduction

The preproprotein encoded by this gene is cleaved into multiple chains that comprise the alpha-2 and delta subunits of the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. Mutations in this gene can cause cardiac deficiencies, including Brugada syndrome and short QT syndrome. Alternate splicing results in multiple transcript variants, some of which may lack the delta subunit portion.

Entrez Gene ID

Human	781
Mouse	12293
Rat	25399
Pig	397377
Guinea pig	100728887

UniProt ID

Human	P54289
Mouse	O08532
Rat	P54290
Pig	Q002C1
Guinea pig	H0VKR3

Alternative Names

CACNA2; CCHL2A; CACNL2A; LINC01112; lncRNA-N3

Function

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling (By similarity).

Biological Process

Calcium ion import across plasma membrane Source: BHF-UCL
Calcium ion transmembrane transport via high voltage-gated calcium channel Source: BHF-UCL
Calcium ion transport Source: UniProtKB
Calcium ion transport into cytosol Source: BHF-UCL
Cardiac muscle cell action potential involved in contraction Source: BHF-UCL
Cellular response to amyloid-beta Source: ARUK-UCL
Membrane depolarization during bundle of His cell action potential Source: BHF-UCL
Positive regulation of high voltage-gated calcium channel activity Source: BHF-UCL
Regulation of calcium ion transmembrane transport via high voltage-gated calcium channel Source: ARUK-UCL
Regulation of calcium ion transport Source: BHF-UCL
Regulation of heart rate by cardiac conduction Source: BHF-UCL
Regulation of membrane repolarization during action potential Source: BHF-UCL
Regulation of ventricular cardiac muscle cell membrane repolarization Source: BHF-UCL

Cellular Location

Membrane

Topology

Extracellular: 25-1073 aa
Helical: 1074-1094 aa
Cytoplasmic: 1095-1103 aa

PTM

Proteolytically processed into subunits alpha-2-1 and delta-1 that are disulfide-linked.

Ye, Y., Barghouth, M., Luan, C., Kazim, A., Zhou, Y., Eliasson, L., ... & Renström, E. (2020). The TCF7L2-dependent high-voltage activated calcium channel subunit α2δ-1 controls calcium signaling in rodent pancreatic beta-cells. Molecular and cellular endocrinology, 502, 110673.

Sun, L., Wang, G., He, M., Mei, Z., Zhang, F., & Liu, P. (2020). Effect and mechanism of the CACNA2D1-CGRP pathway in osteoarthritis-induced ongoing pain. Biomedicine & Pharmacotherapy, 129, 110374.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-CACNA2D1 Recombinant Antibody (20A) (CAT#: CBMAB-1264-CN)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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