Mouse Anti-CANX Recombinant Antibody (CBFYC-0786) (CBMAB-C0841-FY)

Name: Mouse Anti-CANX Recombinant Antibody (CBFYC-0786)
SKU: CBMAB-C0841-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0786

Application

WB, IHC, IF, FC

Immunogen

E.coli-derived human Calnexin/CANX recombinant protein.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	0.25-0.5 μg/ml
IF(ICC)	5 μg/ml
IHC	2-5 μg/ml
FC	1-3 μg/1x10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calnexin

Introduction

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described.

Entrez Gene ID

Human	821
Mouse	12330

UniProt ID

Human	P27824
Mouse	P35564

Alternative Names

Calnexin; Major Histocompatibility Complex Class I Antigen-Binding Protein P88; IP90; P90; CNX

Function

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
Clathrin-dependent endocytosis Source: UniProtKB
Endoplasmic reticulum unfolded protein response Source: GO_Central
Interleukin-27-mediated signaling pathway Source: Reactome
Interleukin-35-mediated signaling pathway Source: Reactome
Protein folding Source: GO_Central
Protein folding in endoplasmic reticulum Source: ParkinsonsUK-UCL
Protein secretion Source: ProtInc
Synaptic vesicle endocytosis Source: UniProtKB
Viral protein processing Source: Reactome

Cellular Location

Endoplasmic reticulum membrane; Endoplasmic reticulum; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232).

Topology

Lumenal: 21-481 aa
Helical: 482-502 aa
Cytoplasmic: 503-592 aa

PTM

Phosphorylated at Ser-564 by MAPK3/ERK1. phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).
Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.
Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.

Zheng, J., Yang, T., Gao, S., Cheng, M., Shao, Y., Xi, Y., ... & Yang, T. (2021). miR‐148a‐3p silences the CANX/MHC‐I pathway and impairs CD8+ T cell‐mediated immune attack in colorectal cancer. The FASEB Journal, 35(8), e21776.

Lam, S. T. T., & Lim, C. J. (2021). Cancer Biology of the Endoplasmic Reticulum Lectin Chaperones Calreticulin, Calnexin and PDIA3/ERp57. Cellular Biology of the Endoplasmic Reticulum, 181-196.

Paskevicius, T., Jung, J., Pujol, M., Eggleton, P., Qin, W., Robinson, A., ... & Agellon, L. B. (2020). The Fabp5/calnexin complex is a prerequisite for sensitization of mice to experimental autoimmune encephalomyelitis. The FASEB Journal, 34(12), 16662-16675.

Guo, X. Y., Liu, Y. S., Gao, X. D., Kinoshita, T., & Fujita, M. (2020). Calnexin mediates the maturation of GPI-anchors through ER retention. Journal of Biological Chemistry, 295(48), 16393-16410.

Forrester, A., De Leonibus, C., Grumati, P., Fasana, E., Piemontese, M., Staiano, L., ... & Settembre, C. (2019). A selective ER‐phagy exerts procollagen quality control via a Calnexin‐FAM 134B complex. The EMBO journal, 38(2), e99847.

Jung, J., Eggleton, P., Robinson, A., Wang, J., Gutowski, N., Holley, J., ... & Michalak, M. (2018). Calnexin is necessary for T cell transmigration into the central nervous system. JCI insight, 3(5).

Jung, J., Wang, J., Groenendyk, J., Lee, D., Michalak, M., & Agellon, L. B. (2017). Fatty acid binding protein (Fabp) 5 interacts with the calnexin cytoplasmic domain at the endoplasmic reticulum. Biochemical and biophysical research communications, 493(1), 202-206.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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