Mouse Anti-CAPN15 Recombinant Antibody (CBXS-2967) (CBMAB-S5715-CQ)

Name: Mouse Anti-CAPN15 Recombinant Antibody (CBXS-2967)
SKU: CBMAB-S5715-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXS-2967

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-119 mapping at the N-terminus of Calpain 15 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calpain 15

Introduction

This gene encodes a protein containing zinc-finger-like repeats and a calpain-like protease domain. The encoded protein may function as a transcription factor, RNA-binding protein, or in protein-protein interactions during visual system development.

Entrez Gene ID

6650

UniProt ID

O75808

Alternative Names

Calpain 15; Small Optic Lobes Homolog; SOLH; Small Optic Lobes (Drosophila) Homolog; Small Optic Lobes Homolog (Drosophila); EC 3.4.22.54; EC 3.4.22.-; Calpain-15; EC 3.4.22;

Biological Process

Proteolysis Source: GO_Central

Cellular Location

Cytoplasm

Zha, C., Farah, C. A., Fonov, V., Rudko, D. A., & Sossin, W. S. (2021). MRI of Capn15 knockout mice and analysis of Capn 15 distribution reveal possible roles in brain development and plasticity. bioRxiv, 763888.

Mor‐Shaked, H., Salah, S., Yanovsky‐Dagan, S., Meiner, V., Atawneh, O. M., Abu‐Libdeh, B., ... & Harel, T. (2021). Biallelic deletion in a minimal CAPN15 intron in siblings with a recognizable syndrome of congenital malformations and developmental delay. Clinical Genetics, 99(4), 577-582.

Zha, C., Farah, C. A., Holt, R. J., Ceroni, F., Al-Abdi, L., Thuriot, F., ... & Sossin, W. S. (2020). Biallelic variants in the small optic lobe calpain CAPN15 are associated with congenital eye anomalies, deafness and other neurodevelopmental deficits. Human Molecular Genetics, 29(18), 3054-3063.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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