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Rat Anti-CASP10 Recombinant Antibody (11J26) (CBMAB-C2538-LY)

This product is antibody recognizes CASP10. The antibody 11J26 immunoassay techniques such as: WB.
See all CASP10 antibodies

Summary

Host Animal
Rat
Specificity
Human
Clone
11J26
Antibody Isotype
IgG2a
Application
WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
0.09% sodium azide
Concentration
0.5 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
caspase 10, apoptosis-related cysteine peptidase
Introduction
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]
Entrez Gene ID
843
UniProt ID
Q92851
Alternative Names
Caspase 10; FAS-Associated Death Domain Protein Interleukin-1B-Converting Enzyme 2; Caspase 10, Apoptosis-Related Cysteine Protease; ICE-Like Apoptotic Protease 4; CASP-10; FLICE2; MCH4; Caspase 10, Apoptosis-Related Cysteine Peptidase; Caspase 10 Apoptosis-Related Cysteine Peptidase;
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.
Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis.
Isoform C is proteolytically inactive.
Biological Process
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Apoptotic process Source: GO_Central
Apoptotic signaling pathway Source: Reactome
Cell surface receptor signaling pathway Source: Reactome
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Regulation of apoptotic process Source: Reactome
Cellular Location
Cytosol; Ripoptosome; CD95 death-inducing signaling complex; Cytoplasm
Involvement in disease
Autoimmune lymphoproliferative syndrome 2A (ALPS2A): A disorder of apoptosis that manifests in early childhood and results in the accumulation of autoreactive lymphocytes. It is characterized by non-malignant lymphadenopathy with hepatosplenomegaly, and autoimmune hemolytic anemia, thrombocytopenia and neutropenia.
Familial non-Hodgkin lymphoma (NHL): Cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Gastric cancer (GASC): A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
PTM
Cleavage by granzyme B and autocatalytic activity generate the two active subunits.

Sargazi, S., Abghari, A. Z., Sarani, H., Sheervalilou, R., Mirinejad, S., Saravani, R., & Eskandari, E. (2021). Relationship Between CASP9 and CASP10 Gene Polymorphisms and Cancer Susceptibility: Evidence from an Updated Meta-analysis. Applied biochemistry and biotechnology, 193(12), 4172-4196.

Pérez, E. M., Shephard, J. L. V., García-Morato, M. B., Marhuenda, Á. R., Nodal, E. M. O., Bozano, G. P., ... & Pena, R. R. (2021). Variants in CASP10, a diagnostic challenge: Single center experience and review of the literature. Clinical Immunology, 108812.

Xia, Y. W., & Wang, S. B. (2021). Long noncoding RNA PVT1 facilitates high glucose‐induced cardiomyocyte death through the miR‐23a‐3p/CASP10 axis. Cell Biology International, 45(1), 154-163.

Kumari, R., Deshmukh, R. S., & Das, S. (2019). Caspase-10 inhibits ATP-citrate lyase-mediated metabolic and epigenetic reprogramming to suppress tumorigenesis. Nature communications, 10(1), 1-15.

Miano, M., Cappelli, E., Pezzulla, A., Venè, R., Grossi, A., Terranova, P., ... & Fioredda, F. (2019). FAS‐mediated apoptosis impairment in patients with ALPS/ALPS‐like phenotype carrying variants on CASP10 gene. British journal of haematology, 187(4), 502-508.

Fioredda, F., Cappelli, E., Mariani, A., Beccaria, A., Palmisani, E., Grossi, A., ... & Miano, M. (2019). Thrombotic thrombocytopenic purpura and defective apoptosis due to CASP8/10 mutations: the role of mycophenolate mofetil. Blood advances, 3(21), 3432.

Tanzer, M. C., Khan, N., Rickard, J. A., Etemadi, N., Lalaoui, N., Spall, S. K., ... & Silke, J. (2017). Combination of IAP antagonist and IFN γ activates novel caspase-10-and RIPK1-dependent cell death pathways. Cell Death & Differentiation, 24(3), 481-491.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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