Mouse Anti-CD177 Recombinant Antibody (4C4) (CBMAB-C6729-LY)

Name: Mouse Anti-CD177 Recombinant Antibody (4C4)
SKU: CBMAB-C6729-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CD177 Recombinant Antibody (4C4) (CBMAB-C6729-LY) in IHC

Immunohistochemical analysis of CD177 expression in human gastric cancer tissue. (a and b) Negative staining (none to weak) for CD177 in a gastric adenocarcinoma. CD177 expression is present only in infiltrating neutrophils while neoplastic cells of well-differentiated (a) or poorly-differentiated (b) carcinoma are negative. Original magnification, ×100 (inset, ×400). (c and d) Note positive (moderate to strong) expression for CD177 in well-differentiated (c) or poorly-differentiated (d) gastric cancer cells. Original magnification, ×100 (inset, ×400). ...View More

Toyoda, T., Tsukamoto, T., Yamamoto, M., Ban, H., Saito, N., Takasu, S., ... & Tatematsu, M. (2013). Gene expression analysis of a Helicobacter pylori-infected and high-salt diet-treated mouse gastric tumor model: identification of CD177 as a novel prognostic factor in patients with gastric cancer. BMC gastroenterology, 13, 1-11. Distributed under Open Access license CC BY 2.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4C4

Application

ELISA, IP, IHC, WB

Immunogen

CD177 (21 a.a. ~ 120 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	3 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD177 Molecule

Introduction

CD177 (CD177 Molecule) is a Protein Coding gene. Diseases associated with CD177 include Polycythemia and Polycythemia Vera. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Innate Immune System.

An important paralog of this gene is TEX101.

Entrez Gene ID

57126

UniProt ID

Q8N6Q3

Function

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407).
In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980).
Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308).
However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681).
Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208).
By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).

Biological Process

Blood coagulation Source: Reactome
Cell adhesion Source: UniProtKB
Cell-cell adhesion via plasma-membrane adhesion molecules Source: UniProtKB
Cell-cell junction maintenance Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Leukocyte cell-cell adhesion Source: UniProtKB
Leukocyte migration Source: Reactome
Neutrophil degranulation Source: Reactome
Neutrophil extravasation Source: UniProtKB
Neutrophil migration Source: UniProtKB
Positive regulation of neutrophil degranulation Source: UniProtKB
Positive regulation of superoxide anion generation Source: UniProtKB
Protein localization to cell surface Source: UniProtKB
Regulation of endocytosis Source: UniProtKB
Regulation of integrin-mediated signaling pathway Source: UniProtKB

Cellular Location

Cell membrane; Secreted; Membrane raft; Cytoplasmic granule membrane; Lamellipodium. Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208). Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980).

PTM

N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).

Browne, T., Wroe, E., Keen, L., & Poles, A. (2021). Human neutrophil antigen 2 sequence‐based typing: Joining the hunt for the CD177 answer. Vox Sanguinis.

Saha, R., Pradhan, S. S., Das, P., Mishra, P., Singh, R., Sivaramakrishnan, V., & Acharya, P. (2021). Inflammatory signature in acute-on-chronic liver failure includes increased expression of granulocyte genes ELANE, MPO and CD177. Scientific reports, 11(1), 1-16.

Dahlstrand Rudin, A., Amirbeagi, F., Davidsson, L., Khamzeh, A., Thorbert Mros, S., Thulin, P., ... & Bylund, J. (2021). The neutrophil subset defined by CD177 expression is preferentially recruited to gingival crevicular fluid in periodontitis. Journal of Leukocyte Biology, 109(2), 349-362.

Lévy, Y., Wiedemann, A., Hejblum, B. P., Durand, M., Lefebvre, C., Surénaud, M., ... & French COVID cohort study group. (2021). CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death. Iscience, 24(7), 102711.

Kim, M. C., Borcherding, N., Ahmed, K. K., Voigt, A. P., Vishwakarma, A., Kolb, R., ... & Zhang, W. (2021). CD177 modulates the function and homeostasis of tumor-infiltrating regulatory T cells. Nature communications, 12(1), 1-13.

Huang, Y. (2019). 4311 Decreased CD177 expression and its association with intravenous immunoglobulin resistance in Kawasaki disease. European Heart Journal, 40(Supplement_1), ehz745-0156.

Deng, H., Hu, N., Wang, C., Chen, M., & Zhao, M. H. (2018). Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA. Arthritis research & therapy, 20(1), 1-10.

Bai, M., Grieshaber-Bouyer, R., Wang, J., Schmider, A. B., Wilson, Z. S., Zeng, L., ... & Nigrovic, P. A. (2017). CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood, The Journal of the American Society of Hematology, 130(19), 2092-2100.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CD177 Matched Antibody Pair (206) (CAT#: APMAB-206LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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