Sign in or Register   Sign in or Register
  |  

Mouse Anti-CD177 Recombinant Antibody (4C4) (CBMAB-C6729-LY)

This product is antibody recognizes CD177. The antibody 4C4 immunoassay techniques such as: ELISA, IP, IHC-P, WB.
See all CD177 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4C4
Antibody Isotype
IgG2a
Application
ELISA, IP, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CD177 Molecule
Introduction
CD177 (CD177 Molecule) is a Protein Coding gene. Diseases associated with CD177 include Polycythemia and Polycythemia Vera. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Innate Immune System.

An important paralog of this gene is TEX101.
Entrez Gene ID
57126
UniProt ID
Q8N6Q3
Alternative Names
CD177 Molecule; CD177 Antigen; Polycythemia Rubra Vera Protein 1; Human Neutrophil Alloantigen 2a; NB1 Glycoprotein; NB1 GP; HNA-2a;
Function
In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407).
In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980).
Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308).
However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681).
Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208).
By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).
Biological Process
Blood coagulation Source: Reactome
Cell adhesion Source: UniProtKB
Cell-cell adhesion via plasma-membrane adhesion molecules Source: UniProtKB
Cell-cell junction maintenance Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Leukocyte cell-cell adhesion Source: UniProtKB
Leukocyte migration Source: Reactome
Neutrophil degranulation Source: Reactome
Neutrophil extravasation Source: UniProtKB
Neutrophil migration Source: UniProtKB
Positive regulation of neutrophil degranulation Source: UniProtKB
Positive regulation of superoxide anion generation Source: UniProtKB
Protein localization to cell surface Source: UniProtKB
Regulation of endocytosis Source: UniProtKB
Regulation of integrin-mediated signaling pathway Source: UniProtKB
Cellular Location
Cell membrane; Secreted; Membrane raft; Cytoplasmic granule membrane; Lamellipodium. Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208). Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980).
PTM
N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).

Browne, T., Wroe, E., Keen, L., & Poles, A. (2021). Human neutrophil antigen 2 sequence‐based typing: Joining the hunt for the CD177 answer. Vox Sanguinis.

Saha, R., Pradhan, S. S., Das, P., Mishra, P., Singh, R., Sivaramakrishnan, V., & Acharya, P. (2021). Inflammatory signature in acute-on-chronic liver failure includes increased expression of granulocyte genes ELANE, MPO and CD177. Scientific reports, 11(1), 1-16.

Dahlstrand Rudin, A., Amirbeagi, F., Davidsson, L., Khamzeh, A., Thorbert Mros, S., Thulin, P., ... & Bylund, J. (2021). The neutrophil subset defined by CD177 expression is preferentially recruited to gingival crevicular fluid in periodontitis. Journal of Leukocyte Biology, 109(2), 349-362.

Lévy, Y., Wiedemann, A., Hejblum, B. P., Durand, M., Lefebvre, C., Surénaud, M., ... & French COVID cohort study group. (2021). CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death. Iscience, 24(7), 102711.

Kim, M. C., Borcherding, N., Ahmed, K. K., Voigt, A. P., Vishwakarma, A., Kolb, R., ... & Zhang, W. (2021). CD177 modulates the function and homeostasis of tumor-infiltrating regulatory T cells. Nature communications, 12(1), 1-13.

Huang, Y. (2019). 4311 Decreased CD177 expression and its association with intravenous immunoglobulin resistance in Kawasaki disease. European Heart Journal, 40(Supplement_1), ehz745-0156.

Deng, H., Hu, N., Wang, C., Chen, M., & Zhao, M. H. (2018). Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA. Arthritis research & therapy, 20(1), 1-10.

Bai, M., Grieshaber-Bouyer, R., Wang, J., Schmider, A. B., Wilson, Z. S., Zeng, L., ... & Nigrovic, P. A. (2017). CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood, The Journal of the American Society of Hematology, 130(19), 2092-2100.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CD177 Recombinant Antibody (4C4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare