Mouse Anti-CD6 Recombinant Antibody (CBT4640) (V2LY-0625-LY2818)

Name: Mouse Anti-CD6 Recombinant Antibody (CBT4640)
SKU: V2LY-0625-LY2818
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CD6 Recombinant Antibody (CBT4640) (V2LY-0625-LY2818) in WB

Mouse Anti-CD6 Recombinant Antibody (CBT4640) (V2LY-0625-LY2818) in IHC

Mouse Anti-CD6 Recombinant Antibody (CBT4640) (V2LY-0625-LY2818) in ELISA

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4640

Application

IHC

Immunogen

Purified recombinant fragment of human CD6 (AA: 472-668) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD6 Molecule

Entrez Gene ID

923

UniProt ID

P30203

Function

Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed:15048703, PubMed:15294938, PubMed:16352806, PubMed:16914752, PubMed:24945728, PubMed:24584089).
Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed:24584089).
Functions as costimulatory molecule; promotes T-cell activation and proliferation (PubMed:15294938, PubMed:16352806, PubMed:16914752).
Contributes to the formation and maturation of the immunological synapse (PubMed:15294938, PubMed:16352806).
Functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed:17601777).
LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed:17601777).
Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed:17601777).

Biological Process

Acute inflammatory response to antigenic stimulus Source: UniProtKB
Adaptive immune response Source: UniProtKB-KW
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Immunological synapse formation Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Lipopolysaccharide-mediated signaling pathway Source: UniProtKB
Positive regulation of cytokine production involved in inflammatory response Source: UniProtKB
Positive regulation of T cell proliferation Source: UniProtKB
Response to lipopolysaccharide Source: UniProtKB

Cellular Location

Cell membrane. Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells (PubMed:15294938, PubMed:16352806). Colocalizes with the TCR/CD3 complex at the immunological synapse (PubMed:15294938).
Soluble CD6: Secreted. The origins of the secreted form are not clear, but it might be created by proteolytic shedding of the ectodomain.

Topology

Extracellular: 18-402
Helical: 403-423
Cytoplasmic: 424-668

PTM

After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
Glycosylated.

Mori, D., Grégoire, C., Voisinne, G., Celis-Gutierrez, J., Aussel, R., Girard, L., ... & Malissen, B. (2021). The T cell CD6 receptor operates a multitask signalosome with opposite functions in T cell activation. Journal of Experimental Medicine, 218(2).

Filgueira, L. M., Cervantes, J. B., Lovelle, O. A., Herrera, C., Figueredo, C., Caballero, J. A., ... & Caballero, A. (2021). An anti-CD6 antibody for the treatment of COVID-19 patients with cytokine-release syndrome: report of three cases. Immunotherapy, 13(4), 289-295.

Kureel, A. K., Saini, S., Singh, B., Singh, K., & Rai, A. K. (2021). Compromised levels of CD6 and reduced T cell activation in the aged immune system. Biomarkers, 1-8.

Dogra, S., & Rajagopalan, M. (2020). Anti-CD6 mAbs for the treatment of psoriasis. Expert Opinion on Biological Therapy, 20(10), 1215-1222.

Velasco-de Andrés, M., Casadó-Llombart, S., Català, C., Leyton-Pereira, A., Lozano, F., & Aranda, F. (2020). Soluble CD5 and CD6: Lymphocytic Class I Scavenger Receptors as Immunotherapeutic Agents. Cells, 9(12), 2589.

Gonçalves, C. M., Henriques, S. N., Santos, R. F., & Carmo, A. M. (2018). CD6, a rheostat-type signalosome that tunes T cell activation. Frontiers in immunology, 9, 2994.

Consuegra-Fernandez, M., Lin, F., Fox, D. A., & Lozano, F. (2018). Clinical and experimental evidence for targeting CD6 in immune-based disorders. Autoimmunity reviews, 17(5), 493-503.

Meddens, M., Mennens, S. F., Celikkol, F. B., Te Riet, J., Kanger, J. S., Joosten, B., ... & Cambi, A. (2018). Biophysical Characterization of CD6—TCR/CD3 Interplay in T Cells. Frontiers in immunology, 9, 2333.

Bughani, U., Saha, A., Kuriakose, A., Nair, R., Sadashivarao, R. B., Venkataraman, R., ... & Nair, P. (2017). T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab. PloS one, 12(7), e0180088.

Enyindah-Asonye, G., Li, Y., Ruth, J. H., Spassov, D. S., Hebron, K. E., Zijlstra, A., ... & Lin, F. (2017). CD318 is a ligand for CD6. Proceedings of the National Academy of Sciences, 114(33), E6912-E6921.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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