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Rat Anti-CD74 Recombinant Antibody (CBXC-1687) (CBMAB-C3533-CQ)

This product is a rat antibody that recognizes CD74. The antibody CBXC-1687 can be used for immunoassay techniques such as: ELISA, FC.
See all CD74 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBXC-1687
Antibody Isotype
IgG1
Application
ELISA, FC

Basic Information

Immunogen
Recombinant protein corresponding to aa56-215 from human CD74, CHO-derived (UniProt # P04441)
Specificity
Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
Lyophilized powder from PBS, Trehalose. Reconstitute in PBS
Concentration
LYOPH
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD74 Molecule
Introduction
CD74 (CD74 Molecule) is a Protein Coding gene. Diseases associated with CD74 include Undifferentiated Pleomorphic Sarcoma and Mantle Cell Lymphoma. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Innate Immune System. Gene Ontology (GO) annotations related to this gene include identical protein binding and amyloid-beta binding.
Entrez Gene ID
UniProt ID
Alternative Names
CD74 Molecule; CD74 Antigen (Invariant Polypeptide Of Major Histocompatibility Complex, Class II Antigen-Associated); CD74 Molecule, Major Histocompatibility Complex, Class II Invariant Chain; HLA-DR Antigens-Associated Invariant Chain; Gamma Chain Of Class II Antigens; Ia-Associated Invariant Chain; MHC HLA-DR Gamma Chain; HLA-DR-Gamma; DHLAG;
Function
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
Class-II-associated invariant chain peptide: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.
Isoform p41: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215).
Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215).
Biological Process
Activation of MAPK activity Source: Ensembl
Antigen processing and presentation Source: GO_Central
Antigen processing and presentation of endogenous antigen Source: UniProtKB
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Cell population proliferation Source: Ensembl
Chaperone cofactor-dependent protein refolding Source: Ensembl
Immunoglobulin mediated immune response Source: UniProtKB
Intracellular protein transport Source: UniProtKB
Leukocyte migration Source: Reactome
Macrophage migration inhibitory factor signaling pathway Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cell migration Source: ARUK-UCL
Negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
Negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: BHF-UCL
Negative regulation of mature B cell apoptotic process Source: Ensembl
Negative regulation of peptide secretion Source: BHF-UCL
Negative regulation of T cell differentiation Source: Ensembl
Negative regulation of viral entry into host cell Source: UniProtKB
Negative thymic T cell selection Source: Ensembl
Positive regulation of B cell proliferation Source: BHF-UCL
Positive regulation of chemokine (C-X-C motif) ligand 2 production Source: BHF-UCL
Positive regulation of chemokine production Source: ARUK-UCL
Positive regulation of cytokine-mediated signaling pathway Source: BHF-UCL
Positive regulation of dendritic cell antigen processing and presentation Source: Ensembl
Positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
Positive regulation of fibroblast proliferation Source: BHF-UCL
Positive regulation of gene expression Source: ARUK-UCL
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: CAFA
Positive regulation of interleukin-6 production Source: ARUK-UCL
Positive regulation of interleukin-8 production Source: ARUK-UCL
Positive regulation of kinase activity Source: CAFA
Positive regulation of macrophage cytokine production Source: BHF-UCL
Positive regulation of macrophage migration inhibitory factor signaling pathway Source: ARUK-UCL
Positive regulation of MAPK cascade Source: CAFA
Positive regulation of monocyte differentiation Source: CAFA
Positive regulation of neutrophil chemotaxis Source: BHF-UCL
Positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
Positive regulation of prostaglandin biosynthetic process Source: ARUK-UCL
Positive regulation of protein phosphorylation Source: CAFA
Positive regulation of T cell differentiation Source: Ensembl
Positive regulation of transcription, DNA-templated Source: CAFA
Positive regulation of type 2 immune response Source: GO_Central
Positive regulation of viral entry into host cell Source: CAFA
Positive thymic T cell selection Source: Ensembl
Prostaglandin biosynthetic process Source: UniProtKB
Protein-containing complex assembly Source: UniProtKB
Protein stabilization Source: UniProtKB
Protein trimerization Source: InterPro
Regulation of macrophage activation Source: UniProtKB
Response to interferon-gamma Source: UniProtKB
T cell activation involved in immune response Source: GO_Central
T cell selection Source: UniProtKB
Cellular Location
Endosome; Trans-Golgi network; Endoplasmic reticulum membrane; Lysosome; Cell membrane. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
Isoform p41: Late endosome; Lysosome
Involvement in disease
A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74-ROS1 chimeric protein.
Topology
Cytoplasmic: 1-46
Helical: 47-72
Extracellular: 73-296
PTM
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

Schoeps, B., Eckfeld, C., Flüter, L., Keppler, S., Mishra, R., Knolle, P., ... & Krüger, A. (2021). Identification of invariant chain CD74 as a functional receptor of tissue inhibitor of metalloproteinases-1 (TIMP-1). Journal of Biological Chemistry, 297(3).

Parkins, A., Skeens, E., McCallum, C. M., Lisi, G. P., & Pantouris, G. (2021). The N-terminus of MIF regulates the dynamic profile of residues involved in CD74 activation. Biophysical Journal, 120(18), 3893-3900.

Chen, E., Reiss, K., Shah, D., Manjula, R., Allen, B., Murphy, E. L., ... & Lisi, G. P. (2021). A structurally preserved allosteric site in the MIF superfamily affects enzymatic activity and CD74 activation in D-dopachrome tautomerase. Journal of Biological Chemistry, 297(3).

Ruvolo, P. P., Hu, C. W., Qiu, Y., Ruvolo, V. R., Go, R. L., Hubner, S. E., ... & Kornblau, S. M. (2019). LGALS3 is connected to CD74 in a previously unknown protein network that is associated with poor survival in patients with AML. EBioMedicine, 44, 126-137.

Meza-Romero, R., Benedek, G., Gerstner, G., Kent, G., Nguyen, H., Offner, H., & Vandenbark, A. A. (2019). Increased CD74 binding and EAE treatment efficacy of a modified DRα1 molecular construct. Metabolic brain disease, 34(1), 153-164.

Liu, Y., Liao, X., & Shi, G. (2019). Autoantibodies in spondyloarthritis, focusing on anti-CD74 antibodies. Frontiers in immunology, 10, 5.

Pantouris, G., Ho, J., Shah, D., Syed, M. A., Leng, L., Bhandari, V., ... & Lolis, E. J. (2018). Nanosecond Dynamics Regulate the MIF‐Induced Activity of CD74. Angewandte Chemie, 130(24), 7234-7237.

Su, H., Na, N., Zhang, X., & Zhao, Y. (2017). The biological function and significance of CD74 in immune diseases. Inflammation Research, 66(3), 209-216.

Al Ssadh, H., Spencer, P. S., Alabdulmenaim, W., Alghamdi, R., Madar, I. H., Miranda-Sayago, J. M., & Fernández, N. (2017). Measurements of heterotypic associations between cluster of differentiation CD74 and CD44 in human breast cancer-derived cells. Oncotarget, 8(54), 92143.

Cheema, P. K., Doherty, M., & Tsao, M. S. (2017). A case of invasive mucinous pulmonary adenocarcinoma with a CD74-NRG1 fusion protein targeted with afatinib. Journal of Thoracic Oncology, 12(12), e200-e202.

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For research use only. Not intended for any clinical use.

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