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Mouse Anti-CENPA Recombinant Antibody (5A7-2E11) (CBMAB-C1151-YY)

This product is mouse antibody that recognizes CENPA. The antibody 5A7-2E11 can be used for immunoassay techniques such as: WB, IF
See all CENPA antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5A7-2E11
Antibody Isotype
IgG1
Application
WB, IF

Basic Information

Immunogen
Synthetic peptide corresponding to a portion of human CENP-A
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Protein G purified
Buffer
1 mg/mL
Concentration
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Centromere Protein A
Entrez Gene ID
1058
UniProt ID
P49450
Alternative Names
Caspase 2; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 2; Protein Phosphatase 1, Regulatory Subunit 57; Protease ICH-1; EC 3.4.22.55; CASP-2; NEDD-2; NEDD2;
Function
Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937).
Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184).
The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239).
May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274).
Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292).
Biological Process
CENP-A containing nucleosome assembly Source: Reactome
Establishment of mitotic spindle orientation Source: UniProtKB
Kinetochore assembly Source: BHF-UCL
Mitotic cytokinesis Source: UniProtKB
Mitotic spindle organization Source: Reactome
Protein localization to chromosome, centromeric region Source: BHF-UCL
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Kinetochore; Centromere. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (PubMed:19114591). Phosphorylation at Ser-68 during early mitosis abolishes association with chromatin and centromeres and results in dispersed nuclear location (PubMed:25556658).
PTM
Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
Trimethylated by NTMT1 at the N-terminal glycine after cleavage of Met-1. Methylation is low before incorporation into nucleosomes and increases with cell cycle progression, with the highest levels in mitotic nucleosomes.
Phosphorylated by CDK1 at Ser-68 during early mitosis; this abolishes association with chromatin and centromeres, prevents interaction with HJURP and thereby prevents premature assembly of CENPA into centromeres (PubMed:25556658). Dephosphorylated at Ser-68 by PPP1CA during late mitosis (PubMed:25556658). Phosphorylation of Ser-7 by AURKA and AURKB during prophase is required for localization of AURKA and AURKB at inner centromere and is essential for normal cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18239465). Initial phosphorylation during prophase is mediated by AURKA and is maintained by AURKB.
Poly-ADP-ribosylated by PARP1.

Liang, Y. C., Su, Q., Liu, Y. J., Xiao, H., & Yin, H. Z. (2021). Centromere Protein A (CENPA) Regulates Metabolic Reprogramming in the Colon Cancer Cells by Transcriptionally Activating Karyopherin Subunit Alpha 2 (KPNA2). The American Journal of Pathology, 191(12), 2117-2132.

Liu, X., Wang, H., & Zhao, G. (2021). Centromere protein A goes far beyond the centromere in cancers. Molecular Cancer Research.

Zhang, S., Xie, Y., Tian, T., Yang, Q., Zhou, Y., Qiu, J., ... & Du, Z. (2021). High expression levels of centromere protein A plus upregulation of the phosphatidylinositol 3‑kinase/Akt/mammalian target of rapamycin signaling pathway affect chemotherapy response and prognosis in patients with breast cancer. Oncology letters, 21(5), 1-12.

Xu, Y., Liang, C., Cai, X., Zhang, M., Yu, W., & Shao, Q. (2020). High centromere protein-a (CENP-A) expression correlates with progression and prognosis in gastric Cancer. OncoTargets and therapy, 13, 13237.

Mahlke, M. A., & Nechemia-Arbely, Y. (2020). Guarding the genome: CENP-A-chromatin in health and cancer. Genes, 11(7), 810.

Swartz, S. Z., McKay, L. S., Su, K. C., Bury, L., Padeganeh, A., Maddox, P. S., ... & Cheeseman, I. M. (2019). Quiescent cells actively replenish CENP-A nucleosomes to maintain centromere identity and proliferative potential. Developmental cell, 51(1), 35-48.

Niikura, Y., Kitagawa, R., & Kitagawa, K. (2019). CENP-A ubiquitylation contributes to maintaining the chromosomal location of the centromere. Molecules, 24(3), 402.

Cao, S., Zhou, K., Zhang, Z., Luger, K., & Straight, A. F. (2018). Constitutive centromere-associated network contacts confer differential stability on CENP-A nucleosomes in vitro and in the cell. Molecular biology of the cell, 29(6), 751-762.

Sathyan, K. M., Fachinetti, D., & Foltz, D. R. (2017). α-amino trimethylation of CENP-A by NRMT is required for full recruitment of the centromere. Nature communications, 8(1), 1-15.

Guo, L. Y., Allu, P. K., Zandarashvili, L., McKinley, K. L., Sekulic, N., Dawicki-McKenna, J. M., ... & Black, B. E. (2017). Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition. Nature communications, 8(1), 1-15.

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For research use only. Not intended for any clinical use.

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