Mouse Anti-CEP350 Recombinant Antibody (EG649) (CBMAB-EN782-LY)

Name: Mouse Anti-CEP350 Recombinant Antibody (EG649)
SKU: CBMAB-EN782-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG649

Application

IF, IHC

Immunogen

Recombinant protein corresponding to Human CEP350.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	2-10 μg/ml
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2, 40% glycerol

Preservative

0.02% sodium azide

Concentration

0.5 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Centrosomal Protein 350

Introduction

The product of this gene is a large protein with a CAP-Gly domain typically found in cytoskeleton-associated proteins. The encoded protein primarily localizes to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. The encoded protein directly interacts with another large centrosomal protein and is required to anchor microtubules at the centrosome. It is also implicated in the regulation of a class of nuclear hormone receptors in the nucleus. Several alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Entrez Gene ID

9857

UniProt ID

Q5VT06

Alternative Names

Centrosomal Protein 350; Centrosomal Protein 350kDa; CAP350; Centrosome-Associated Protein Of 350 KDa; Centrosome Associated Protein 350; Centrosome-Associated Protein 350; KIAA0480; Cep350; GM133;

Function

Plays an essential role in centriole growth by stabilizing a procentriolar seed composed of at least, SASS6 and CENPJ (PubMed:19052644).
Required for anchoring microtubules to the centrosomes and for the integrity of the microtubule network (PubMed:16314388, PubMed:17878239, PubMed:28659385).
Recruits PPARA to discrete subcellular compartments and thereby modulates PPARA activity (PubMed:15615782).
Required for ciliation (PubMed:28659385).

Biological Process

Microtubule anchoring Source: InterPro
Non-motile cilium assembly Source: Ensembl
Protein localization to centrosome Source: Ensembl

Cellular Location

Centrosome; Spindle; Centriole; Cilium basal body; Nucleus. Associated with mitotic spindles (PubMed:16314388). Nuclear, in discrete foci. Associated with intermediate filaments (PubMed:15615782). Also present in the pericentrosomal area (PubMed:17878239). Localizes on both mother and daughter centrioles. Localizes to an axial position on the mother centriole (PubMed:28625565). Localizes to the distal end of the centriole on the subdistal appendage region (PubMed:28659385).

PTM

Phosphorylated during mitosis.

Gonçalves, A. B., Hasselbalch, S. K., Joensen, B. B., Patzke, S., Martens, P., Ohlsen, S. K., ... & Pedersen, L. B. (2021). CEP78 functions downstream of CEP350 to control biogenesis of primary cilia by negatively regulating CP110 levels. bioRxiv, 2020-10.

Ghosh, S., Chakraborty, J., Goswami, A., Bhowmik, S., Roy, S., Ghosh, A., ... & Banerjee, S. (2020). A novel microRNA boosts hyper-β-oxidation of fatty acids in liver by impeding CEP350-mediated sequestration of PPARα and thus restricts chronic hepatitis C. RNA biology, 17(9), 1352-1363.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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