Mouse Anti-CLIC6 Recombinant Antibody (CBWJC-2800) (V2LY-1206-LY231)

Name: Mouse Anti-CLIC6 Recombinant Antibody (CBWJC-2800)
SKU: V2LY-1206-LY231
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-2800

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 327-351 within an internal region of CLIC6 of mouse.

Host Species

Mouse

Specificity

Mouse

Antibody Isotype

IgG3, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chloride Intracellular Channel 6

Entrez Gene ID

54102

UniProt ID

Q96NY7

Function

May insert into membranes and form chloride ion channels. May play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport (By similarity).

Biological Process

Chloride transport Source: UniProtKB-KW
Regulation of ion transmembrane transport Source: UniProtKB-KW

Cellular Location

Cytoplasm; Cell membrane. Predominantly cytoplasmic. Upon chloride ion efflux from the cell, it is translocated to the plasma membrane (By similarity).

Topology

Helical: 489-509

PTM

Phosphorylated.

Ferofontov, A., Marom, M., Giladi, M., & Haitin, Y. (2019). Inherent Flexibility and Oligomerization of CLIC5A and CLIC6-A Comparative Static and Dynamic Structural Study. Biophysical Journal, 116(3), 50a.

Ferofontov, A., Strulovich, R., Marom, M., Giladi, M., & Haitin, Y. (2018). Inherent flexibility of CLIC6 revealed by crystallographic and solution studies. Scientific reports, 8(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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