Rabbit Anti-CNOT3 Recombinant Antibody (CBWJC-2865) (V2LY-1206-LY461)

Name: Rabbit Anti-CNOT3 Recombinant Antibody (CBWJC-2865)
SKU: V2LY-1206-LY461
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBWJC-2865

Application

IP, WB

Immunogen

Synthetic peptide corresponding to human CNOT3 at C-terminal.

Host Species

Rabbit

Specificity

Human, Monkey, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000
IP	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100ug/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CCR4-NOT Transcription Complex Subunit 3

Entrez Gene ID

Human	4849
Mouse	232791
Rat	308311
Monkey	693292

UniProt ID

Human	O75175
Mouse	Q8K0V4
Rat	D3ZUV9
Monkey	H2R0F5

Function

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.

Biological Process

DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
Gene silencing by RNA Source: UniProtKB-KW
Nuclear-transcribed mRNA poly(A) tail shortening Source: GO_Central
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Regulation of stem cell population maintenance Source: UniProtKB
Regulation of transcription, DNA-templated Source: InterPro
Regulation of translation Source: UniProtKB-KW
Trophectodermal cell differentiation Source: Ensembl

Cellular Location

Cytoplasm; Nucleus; P-body. NANOS2 promotes its localization to P-body.

Involvement in disease

Intellectual developmental disorder with speech delay, autism and dysmorphic facies (IDDSADF):
An autosomal dominant disorder characterized by mild to severe intellectual disability, developmental delay, delayed or absent speech, hypotonia, short stature, autistic features, and highly variable dysmorphic facial features.

Mao, Q., Zhuang, Q., Shen, J., Chen, Z., Xue, D., Ding, T., & He, X. (2021). MiRNA-124 regulates the sensitivity of renal cancer cells to cisplatin-induced necroptosis by targeting the CAPN4-CNOT3 axis. Translational Andrology and Urology, 10(9), 3669.

Jing, L., Zhai, M. E., Cui, J., Fan, X. Y., Cheng, Y. Y., Jiang, J. L., & Chen, Z. N. (2019). CNOT3 contributes to cisplatin resistance in lung cancer through inhibiting RIPK3 expression. Apoptosis, 24(7), 673-685.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-CNOT3 Recombinant Antibody (4B8) (CAT#: V2LY-1206-LY459)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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